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Fluorescence spectra
Introduction
This topic shows sample emission spectra from common fluorochromes, as well as the more common laser
excitation lines. This information is useful for designing multicolor panels. An interactive fluorescence viewer is
available at
bdbiosciences.com . You can also look for training by selecting Support > Training.
Designing multicolor panels
The BD FACSymphony™ A1 flow cytometer is designed specifically for multicolor research. Results depend on
the excitation and emission spectra of the individual dye, the number of fluorescently labeled binding sites on
the cell, as well as spectral overlap and spillover to other detectors. For more information about designing
multicolor panels, see
Selecting Reagents for Multicolor Flow Cytometry (Part No. 23-9538-02).
Example laser and dye interactions
The following figures show the emission spectra of some common dyes, based on laser excitation. In many
cases, a given dye can be excited by multiple laser wavelengths, yielding different emission intensities.
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98 BD FACSymphony™ A1 Flow Cytometer User's Guide