BD FACSymphony A1 Flow, Руководство пользователя

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Optics
Introduction
This topic describes the optical components for the BD FACSymphony™ A1 flow cytometer including:
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Detector arrays
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Lasers
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Optical filters
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Signal detectors
Detector arrays
The BD FACSymphony™ A1 detector arrays consist of polygons. Each polygon can be configured with one to six
fluorescent detectors. The configuration of detectors may limit the actual number of detectors on each
polygon. To access the red laser detector array, see
Accessing the red laser detector array (page 49) .
Lasers
The BD FACSymphony™ A1 flow cytometer is configured with four lasers as listed in the following table.
Laser Wavelength (nm) Power (mW)
Blue 488 100
Red 637 100
Violet 405 100
Yellow Green 561 100
Optical filters
Optical filters attenuate light or help direct it to the appropriate detectors. The name and spectral
characteristics of each filter appear on its holder.
There are two types of optical filters in the BD FACSymphony™ A1:
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Longpass dichroic filters (LPs) Transmit wavelengths at or longer than the specified value and reflect all
light below the specified wavelength.
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Bandpass filters (BPs) Pass a narrow spectral band of light.
When dichroic filters are used as steering optics to direct different color light signals to different detectors, they
are called dichroic mirrors. Longpass dichroic mirrors transmit longer wavelengths to one detector while
reflecting shorter wavelengths to a different detector.
The BD FACSymphony™ A1 flow cytometer polygon detector arrays use dichroic longpass mirrors on the inside,
and bandpass filters on the outside of the filter holders.
22 BD FACSymphony™ A1 Flow Cytometer User's Guide