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BD FACSort System

337452 Rev. A

 

BD FACSort System

 

After completing this module, you will be able to:

•

Describe the BD FACSort™ system and how it works.

•

Perform instrument startup and shutdown procedures.

•

Describe instrument maintenance procedures.

Содержание FACSort

Страница 1: ...337452 Rev A BD FACSort System After completing this module you will be able to Describe the BD FACSort system and how it works Perform instrument startup and shutdown procedures Describe instrument maintenance procedures ...

Страница 2: ...ions immunophenotyping and DNA cell cycle analysis In addition the BD FACSort flow cytometer allows you to identify and sort isolate a subpopulation from your sample The BD FACSort system consists of a sensor module Figure 3 1 computer module and several software packages Many of the cytometer functions are controlled by the software Cytometer Figure 3 1 BD FACSort Sensor Module Power Switch This ...

Страница 3: ...red STANDBY stops sheath flow and lowers laser power to conserve sheath fluid and prolong laser life Hard STANDBY When the fluid control dial is manually turned to STANDBY all fluid valves are closed except for one valve that vents the sample test tube air pressure to the atmosphere Additionally the laser input current is reduced to a standby level Auto STANDBY If the instrument is in RUN and the ...

Страница 4: ...luidics drawer the lower left panel of the instrument slides out for easy access to the fluidics reservoirs and sheath filter Sheath Reservoir This 4 liter container located on the left holds enough sheath fluid for approximately 3 hours of run time It is equipped with a low level fluid detector which indicates via software a near empty condition A metal bracket fits over the reservoir to secure t...

Страница 5: ... the direction of the arrow relieves the sheath reservoir of air pressure This allows for the removal of the reservoir when refilling Air Filter This component filters the air that cools the laser Optics Door This door shields the compartment which houses the flow cell assembly Open this door to view the flow cell while draining and filling Flow Cell This quartz cuvette is where the laser intercep...

Страница 6: ... aspirate fluid and prevent drips from splashing onto the countertop Figure 3 3 Sample Injection Tube and Droplet Containment System Tube Support Arm This arm supports the sample tube The arm has three positions centered below the sample tube to the right or to the left of the sample tube Its main function is to activate the droplet containment vacuum The vacuum is activated when the arm is in the...

Страница 7: ...d having the sample aspirated and removed to the waste reservoir Collection Station The area that houses the collection tubes Collection Tubes The collection tubes are located in the collection station Three 50 mL conical tubes can be installed in the collection station The conical tubes are used to collect the cells after they are sorted Sort Line The sort lines are tubings that deliver the sorte...

Страница 8: ...urn on the cytometer The power switch is located in the upper left corner of the instrument 2 Turn on the computer 3 Choose your user ID and enter the password Filling the Sheath Tank Recommended Sheath Fluid BD FACSFlow sheath fluid BD Biosciences for nonsorting applications Phosphate buffered saline PBS Dulbecco s Ca2 and Mg2 free for sorting applications Non recommended Sheath Fluids Fisher Hem...

Страница 9: ... pressurized fluid may be forced into the air supply tubing preventing proper pressurization Additionally since there is approximately 400 mL of bleach in the 4 L waste tank filling the sheath tank to maximum capacity will overfill the waste tank 6 Replace the sheath tank 7 Replace the sheath bracket by sliding the metal bracket toward you Make sure that the square lip on the right side of the bra...

Страница 10: ...ginal positions Insert the waste fluid detection probe connector into its housing Insert the orange quick disconnect into the orange colored port until you hear a click Insert the white quick disconnect into the white colored port until you hear a click Verify there are no kinks in the waste and air vent tubing lines 7 Check the sheath filter to be sure that no air bubbles are trapped inside If bu...

Страница 11: ... iodide PI acridine orange AO or thiazole orange TO Cleaning with 10 Bleach 1 Set the fluid control to RUN install a tube containing 3 mL of a bleach solution on the SIP with the support arm to the side vacuum is on and let it run for 1 minute Use BD FACS clean solution or a 1 10 dilution of bleach in DI water as the bleach solution BD FACS clean solution is a bleach based cleaning agent for daily...

Страница 12: ...o maintain optimal instrument performance Monthly cleaning Sheath filter replacement Air filter cleaning Bal seal replacement Sample O ring replacement The following section describes the monthly cleaning procedure Other procedures can be found in the BD FACSort User s Guide Monthly Cleaning Perform overall system fluidics cleaning at least once a month or more frequently if you are running high v...

Страница 13: ... bleach in DI water as the bleach solution 6 Press the HI sample flow rate button then install a tube containing 3 mL of bleach solution on the SIP 7 Press the RUN fluid control button and allow the solution to run for 20 to 30 minutes 8 Remove the tube of bleach solution from the SIP OPTIONAL Repeat steps 5 through 8 using BD FACS rinse solution BD FACS rinse solution is a detergent based cleanin...

Страница 14: ... should remain on the SIP to prevent salt deposits from forming in the injection tube NOTICE You will need to run sheath fluid for approximately 5 minutes before acquiring samples If you will not be using your instrument for a week or longer perform monthly maintenance and keep the distilled water in the fluidic system until you use the instrument again Contrad 70 is an excellent solution for remo...

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