BD FACSCanto II, Справочное руководство, Страница: 18 / 158

18 BD FACSCanto II Flow Cytometer Reference Manual
When you install tubes onto the SIT, a pump within the fluidics cart pressurizes
the plenum, which then provides sheath fluid to the flow cell. At the same time,
the sample tube is pressurized and sample is pushed up the SIT and into the flow
cell.
When you remove tubes from the SIT, the cytometer cleans the SIT by flushing
sheath solution down the inside and outside of the tube. The flushed sheath
solution is aspirated by the aspirator arm.
SIT cleaning between tubes is automatic when you use BD FACSCanto clinical
software. In BD FACSDiva software, SIT cleaning between tubes is automatic
unless you disable it by deselecting the SIT Flush checkbox on the Acquisition
Dashboard.
Do not leave a tube of distilled water on the SIT between sample tubes, or during
or after daily shutdown.
Never install a tube onto the SIT that contains more than 3 mL of fluid. This will
cause the tube sensor to become wet and fail to properly sense tubes.
Flow Cell
Once the sample moves into the flow cell, particles move in single file through the
laser beams. The scattered and emitted light from these particles provides
information about their size, shape, granularity, and fluorescence properties.
The flow cell is behind the flow cell access door. For more information, see
Fluidics System on page 106.