Azure Cielo
™
Dx
Real-Time PCR Systems
User Manual
Part Numbers AIQ314 and AIQ315
UM-0008 R3
Страница 1: ...Azure Cielo Dx Real Time PCR Systems User Manual Part Numbers AIQ314 and AIQ315 UM 0008 R3...
Страница 2: ...st electric shock in case of a fault is located on the inside of the back panel NOTE This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15...
Страница 3: ...nted against defects in materials and workmanship for one year unless otherwise outlined on your sales order or www azurebiosystems com warranty If any defect occurs in the instrument during this warr...
Страница 4: ...f um Informationen hinsichtlich der Entsorgung Ihres Ger tes zu erhalten ITA Questo simbolo indica che i rifiuti derivanti da apparecchiature elettriche ed elettroniche non devono essere smaltiti come...
Страница 5: ...Azure Cielo Dx Real Time PCR system to PC via Azure Cielo Dx Manager software 9 3 Device Software Overview 10 3 1 The Home Screen 10 3 2 Experiment Setup 10 3 2 1 Protocol Manager 11 3 2 2 Change Prot...
Страница 6: ...96 Reaction volume 10 150 L 10 150 L Excitation source LED LED Detection channels 3 6 Multiplexing Up to 3 targets Up to 6 targets Thermal element Peltier Peltier Max block ramp rate 6 C sec 6 C sec...
Страница 7: ...qPCR plates with rim are NOT compatible with the Cielo Dx system Plate Notch The notch is a modified corner of a qPCR plate which is determined by the instrument being used for optimal fit The notch m...
Страница 8: ...placed away from interfering electrical signals and magnetic fields If possible a dedicated electrical outlet should be used to eliminate electrical interference from other instrumentation in your lab...
Страница 9: ...NETWORK Wi Fi CONNECTION INFO 2 4 Connect Azure Cielo Dx Real Time PCR system to PC via Azure Cielo Dx Manager software A Internet connection 1 Please make sure both Azure Cielo Dx Real Time PCR syste...
Страница 10: ...3 2 Data Manager Access run data see Section 3 3 Device Settings Access device settings see Section 3 4 Connection Bar Status of internet connection Table 1 Buttons in the home screen 3 2 Experiment S...
Страница 11: ...Connection Bar Status of internet connection Sort Sort in ascending or descending order Add Add User Add Protocol Delete Delete User Delete Protocol Next Go to the PROPERTIES screen to edit the proto...
Страница 12: ...can see the new user added to the PROTOCOL MANAGER screen Figure 7 Add New User process flow C Add New Protocol Tap symbol to add a new protocol Tap on Add Protocol to add a new user Enter the name fo...
Страница 13: ...ete all existing protocol and data stored under that user The system will prompt you to confirm the deletion Tap OK to delete Figure 10 Delete exisiting user or protocol in PROTOCOL MANAGER 3 2 2 Chan...
Страница 14: ...OTHER button to customize reaction volume Lid Temperature Select lid temperature Off lid heating Turn off lid heating Light Source Select light source s Back Back to PROTOCOL MANAGER screen Next Go t...
Страница 15: ...he wells Press to save changes on the well plate and return to PROPERTIES screen Note 2 column of wells are deselected per tap Tap to proceed to RUN SETUP screen Figure 13 WELL SELECTION screen Figure...
Страница 16: ...will be sent when the protocol run is successfully completed 3 2 5 Run Setup Figure 17 RUN SETUP screen Button Button Name Function Home Back to Home Screen Connection Bar Status of internet connecti...
Страница 17: ...a step Tap on this icon to select the step which must be deleted Go To module Duplicate existing cycling steps in the RUN SETUP Save Saving Run Setup Settings Camera and or Gradient PCR Advance settin...
Страница 18: ...The number on the button displays the number of cycles Tap on to enter the number of cycles you want to run Tap on to enter the step number you want the cycle to repeat everytime after the last step...
Страница 19: ...s The switch button turns BLUE when the camera is ON and GRAY when the camera is OFF Figure 19 Advance settings screen for camera and or gradient PCR The camera at a particular step is active if the i...
Страница 20: ...Melt Curve Tap to access advance setting for Melt Curve Adjust starting temperature ending temperature resolution and scan Tap to save the modified Melt Curve advance settings Figure 20 Melt Curve adv...
Страница 21: ...button 3 2 6 Run Protocol Figure 22 The screen display after qPCR run finished Button Button Name Function Home Back to Home Screen Connection Bar Status of internet connection Stop Stop the run Paus...
Страница 22: ...time the user wishes to pause the experiment tap The button changes to Tap the green button again to continue the experiment run The progress bar at the bottom of the screen displays the percentage of...
Страница 23: ...Table 6 Buttons in the DATA MANAGER screen A Access run data Amplification Curve and Temperature Curve Select the User Protocol then the Data name to access the Amplification Curve and Temperature Cur...
Страница 24: ...to display or hide the amplification traces detected under the respective channel Tap the button to go back to the DATA MANAGER screen 2 Temperature Curve Tap the Temperature Curve image under PREVIEW...
Страница 25: ...periment files will be deleted Tap OK to confirm the delete C Delete Protocol Select the protocol under PROTOCOL list Tap button to delete the selected protocol Note The selected protocols and all ass...
Страница 26: ...tant Make sure that 1 Cielo Dx device is successfully connected to the Internet To connect the Cielo Dx Device to the internet please refer Section 2 3 Connect Azure Cielo Dx Real Time PCR system to i...
Страница 27: ...me is also visible and this can be reset to the users choices Green color in the system status indicate that the device is ready to run Figure 29 Device Info under GENERAL tab DEVICE SETTINGS screen D...
Страница 28: ...twork Email 3 Enter the email settings based on email provider These settings can be obtained from your IT Administration 4 Some examples of common server settings are listed in the table below Email...
Страница 29: ...Server outlook office365 com Port 993 Encryption SSL TLS Server outlook office365 com Port 995 Encryption SSL TLS Server smtp office365 com Port 587 Encryption SSL TLS Yahoo Server imap mail yahoo com...
Страница 30: ...r can add a custom dye put a dye name and choose a dye channel to calibrate with For new or custom dye calibration please make sure your plate layout follows the template in Figure X Dye reagent conce...
Страница 31: ...g Question Answer What is the best practice for new or custom dye calibration Best with 2 fold dilutions minimum 20 l of reaction volume and assign the stock dye reagent as the highest concentration i...
Страница 32: ...ure Biosystems logo Azure Biosystems and Cielo are trademarks of the Company All other trademarks service marks and trade names appearing in this brochure are the property of their respective owners U...
