Document 4400Q902 version 1.02
Page 15 of 18
RX2000 User Manual
11.
Set the main valves to the loading position and reload the drive syringes with reagents.
12.
Set the exhaust valve to open the stopping syringe to the waste reservoir, the indicator arrows should
point upwards and to the left, and push the stopping syringe piston fully forward.
13.
Set the exhaust valve to connect the stopping syringe to the main flow line - indicator arrows should
point downwards and to the left.
14.
Set the position of the stopping-bar and microswitch, located adjacent to the stopping syringe, according
to the total reagent volume required on each drive (i.e. between 0.3 ml and 0.5 ml).
15.
Set the main valves to the drive position and push the drive bar firmly but smoothly.
16.
Return the main valves to the loading position so as to isolate the drive syringes from the flow line (this
ensures that previously mixed solution cannot be passed back up the flowline beyond the mixer) and
then set the exhaust valve to connect the stopping syringe to the waste reservoir syringe. Discharge the
contents of the stopping syringe into the waste reservoir.
17.
Prepare for another drive (i.e. reset the waste valve so that the stopping syringe is connected to the main
flow line only and reset the main valves to the drive position).
3.3
Operation using the pneumatic drive accessory
The RX2000 pneumatic accessory uses compressed gas to drive the drive syringes and could
trap hands or clothing, causing injury to the user. Keep hands and clothing clear when
performing a drive using the accessory.
The pneumatic drive assembly comprises a baseplate mounted control box with a pressure regulator, push
button valve and linear actuator. It should be set up and operated as follows:-
1.
Mount the RX2000 Stopped-Flow Mixing Accessory onto the baseplate using the four fixing holes
provided (the feet on the base of the Mixing Accessory should not be removed before mounting).
2.
Connect the pneumatic control unit to a suitable source of compressed gas such as air or nitrogen. If a
gas bottle is used, a regulator which can deliver 3 to 4 bar (45 to 60 p.s.i.) will be required.
3.
The pneumatic control unit is fitted with a variable pressure regulator with an upper setting of 4 bar (60
p.s.i.). The Mixing Accessory can be operated safely up to this pressure.
4.
Push back the actuator piston fully by hand and load the Mixing Accessory drive syringes with reagents
as per usual. Ensure that the actuator push rod is in contact with the Mixing Accessory drive mechanism
(pull forward the actuator push rod by hand if necessary). Set the Mixing Accessory flowline valves to the
drive position and press the push button valve to energise the drive. For longer reactions, it may be
advisable to keep the pressure applied until the reaction course is complete.