Applied Biosystems GeneAmp PCR System 9700, Руководство пользователя

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5-12 Creating and Editing Methods
Modifying Ramp
Rates
The ramp time is the time it takes the instrument to change from one temperature to
another. Using functions accessible from the Modify screen, you can modify the
up-ramp and down-ramp rates of the instrument by defining it as a percentage of the
temperature’s maximum rate of increase. The default maximum up-ramp and
down-ramp rates is 100%.
6 You can take the following action:
An asterisk * appears on method screens for parameters that have been modified.
7 Press Accept to accept all entries on the AutoX screen.
To automatically change temperature control parameters:
(continued)
Step Action
If you want to... Then press...
increase the value every cycle
(a plus sign displays in the current field)
+ (plus sign).
decrease the value every cycle
(a minus sign displays in the current field)
- (minus sign).
To modify ramp rates:
Step Action
1 Press Modify from the Modify screen. The Select Modification screen appears.
2 Press
Ramp
. The
Ramp Rate Modification
screen appears.
In this example, you can modify the rate at which the instrument ramps up from
72.0 °C to 94.0 °C, down from 94.0 °C to 55.0 °C, or up from 55.0 °C to 72.0 °C.
3 Use the Circular Key Pad to select a ramp that you want to modify.
4 Move the highlight box to the third temperature in the cycling segment of the default
method to modify the highlighted ramp rate.
The third ramp rate defines the rate at which the instrument increases from 55.0 °C
to 72.0 °C each time the method cycles.
2 Pre-PCR 3 Tmp 25 Cycles 2 Holds
55.0
100%
72.0
100%
94.0
100%
F1 F3 F4 F5 F2
Accept Cancel Slower Faster
2 Pre-PCR 3 Tmp 25 Cycles 2 Holds
55.0
100%
72.0
94.0
100%
100%
F1 F3 F4 F5 F2
Accept Cancel Slower Faster