Applied Biosystems GeneAmp PCR System 9700, Руководство пользователя

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Creating and Editing Methods 5-11
Modifying Cycling
Introduction
In addition to customizing values for PCR temperature control parameters, you can
use the More function on the create screen and access cycle modification functions
that allow you to:
♦
Auto-increment/decrement time and temperature parameters.
♦
Modify up-ramp and down-ramp rates in the cycling segment of a method.
♦
Insert holds, cycles, and programmed pauses.
♦
Delete temperature control parameters.
The time or temperature parameter you select on the create screen, determines which
modification function you can access when you press More. Different modification
functions are available depending on whether you select a temperature control
parameter in the pre-PCR segment, the PCR segment, or the post-PCR segment of
a method.
Changing
Temperature
Control Parameters
Using the AutoX function, you can automatically increase or decrease the value for
any PCR segment parameter by a fixed amount every cycle.
Note This feature is particularly useful towards the end of the amplification process since the
amount of PCR product, available to be extended, increases with the number of cycles while the
amount of available enzyme remains constant.
To automatically change temperature control parameters:
Step Action
1 From the
Create
screen, use the Circular Key Pad to select a time or temperature
parameter in the PCR segment.
2 Press
More
.
The Modify screen appears.
3 Press
Modify
.
The
Select Modification
screen appears.
4 Press
AutoX
. The
AutoX
screen appears.
Note If you have inserted a programmed pause, the AutoX screen displays the
pause, but you cannot modify it from the AutoX screen.
5 Select the PCR time or temperature parameter that you want modified when you
run your method.
Note From the AutoX screen, you cannot modify the number of parameters in
each segment or the number of cycles.
2 Pre-PCR 3 Tmp 25 Cycles 2 Holds
+1.0
-0:30
+0.0
+0:00
+0.0
+0:00
F1 F3 F4 F5 F2
Accept Cancel - +
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