4-6 Running PCR Samples
Running a Method
Starting a Run
After choosing a method, follow these steps to start a run.
To start a run:
Step
Action
1
From the
Main
menu, press
Run
to display stored methods.
2
Use the up and down Circular Key Pad to select the method.
3
a. Press
Start
.
The
Select Method Options
screen appears.
b. Choose from the following options:
4
If the displayed reaction volume differs from your reaction volume, enter the volume
of your reactions in the
Reaction Volume
field.
The reaction volume range is dependent on the mode selected.
Note
Each method remembers the reaction volume.
Press the
CE
key to clear an entry.
96-Well Gold
Sample Block
Module
Select
9600
or
MAX
mode first, and then select
volume
.
96-Well
Aluminum
Sample Block
Module
Select
9600
or
STD
mode first, and then select
volume
.
Select Method Options
Enter a value from 5 to 50 µL
Reaction Volume: 50 µL
Ramp speed: 9600
F1
F3
F4
F5
F2
Start
Cancel
9600
MAX
Select Method Options
Enter a value from 5 to 50 µL
Reaction Volume: 50 µL
Ramp speed: 9600
F1
F3
F4
F5
F2
Start
Cancel
9600
STD
For...
The range is...
9600 mode
5 to 50 µL.
MAX/STD mode
5 to 100 µL.
Содержание GeneAmp PCR System 9700
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