Chapter 4
Performing the Regions of Interest (ROI) Calibration
Performing the ROI Calibration
46
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
7.
Repeat
for the remaining
filters: Filter B, Filter C, and Filter D (and Filter
E for 7500 and 7500 Fast instruments).
Reset the Exposure Time to
2048
before
performing the calibration for each filter.
The ROI calibration is complete when Masks
Loaded for all the Filters displays OK.
8.
Click
to close the ROI Inspector.
9.
In the SDS software, select
File Close
.
10.
When prompted to save the plate document,
click
.
Unloading the Plate
PHYSICAL INJURY
HAZARD.
During instrument operation, the sample
block can be heated to 100 °C. Before performing the
following procedure, be sure to wait until the sample
block reaches room temperature.
.
1.
In the ROI Inspector dialog box,
click
.
2.
Press the tray door to open it.
Note:
If you cannot open the tray door, the
sample block may be in its raised position,
locking the tray position. To lower the block,
click
.
Click
Calibration complete
when Masks Loaded is
OK for all Filters
Select
a
a
