Applied Biosystems 3730xl, Руководство пользователя

Страница: 87 / 170

Run module parameters
Parameter Name
Range Comment
Oven_Temperature 18 ℃ –70 ℃ Temperature setting for the main oven throughout the run.
PreRun_Voltage 0–15 kV Pre run voltage setting before sample injection.
PreRun Time 1–1,800 seconds Prerun voltage time.
Injection_Voltage 0–15 kV Injection voltage setting for sample injection.
Injection_Time 1–90 seconds Sample injection time.
First_ReadOut_time 100–16,000
milliseconds
The interval of time for a data point to be produced.
First_ReadOut_time should be equal to
Second_ReadOut_time.
Second_ReadOut_Time 100–16,000
milliseconds
The interval of time for a data point to be produced.
Second_ReadOut_time should be equal to
First_ReadOut_time.
Run_Voltage 0–15 kV Final run voltage.
Voltage_Number_Of_Steps
[1]
0–100 steps Number of voltage ramp steps to reach Run_Voltage.
Voltage_Step_Interval
[1]
0–180 seconds Dwell time at each voltage ramp step.
Voltage_Tolerance
[1]
0.1–6 kV Maximum allowed voltage variation.
Current_Stability
[1]
0–2,000 μA Maximum allowed electrophoresis current variation. Current
fluctuations above this value will be attributed to air bubbles in
system and the voltage automatically powered off.
Ramp_Delay
[1]
1–1,800 seconds Delay During Voltage Ramp.
Data_Delay 1–1,800 seconds Time from the start of separation to the start of sample data
collection.
Run_Time 300–14,000 seconds Run time during which data is collected after Ramp_Delay.
[1]
Do not change this value unless you are instructed to do so by Support.
Overview of analysis protocols (sequence analysis)
An analysis protocol contains all the settings that are necessary for sequence analysis
with Sequencing Analysis Software or SeqScape ™ Software.
• Basecalling settings—The basecaller, DyeSet file, and analysis stop point to be
used.
• Mixed Bases—(Optional) Mixed base identification and the percent value of the
second highest to the highest peak.
• Clear Range—The clear range to be used based on base positions, sample quality
values, and/or number of ambiguities (Ns) present.
Chapter 7 Create and manage instrument protocols, run modules, and analysis protocols
Overview of analysis protocols (sequence analysis)
7
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide 87