Applied Biosystems 3730xl, Руководство пользователя

Страница: 111 / 170

Dye set G5 created from matrix standard set DS-33
• Best practice is to perform a spectral calibration every time you install a new
capillary array.
• There may be circumstances where you want to reuse a calibration that was
generated using a different capillary array.
Note: You must select the calibration before data is collected. After data is
collected, you cannot reapply a different spectral calibration.
• Do not reuse a previous calibration when you use the G5-RCT dye set. Because of
the way the spectral calibration is generated, it is specific to the capillary array
used to generate the calibration.
1.
In the navigation pane of the Data Collection Software, select GA
Instruments
4
ga3730
4
Instrument name
4
Spectral Viewer.
Failed calibrations are marked with an asterisk *.
2. Select the dye set of interest.
3. Click the List of Calibrations list in the lower right-pane.
Example of a
passing fragment
analysis spectral
calibration
Select a previous
calibration as the
active spectral
calibration
Chapter 8 Calibrate the instrument
Perform spectral calibration for sequencing and fragment analysis
8
3730xl DNA Analyzer with 3730xl Data Collection Software 5 User Guide 111