Operator’s Manual
Rev 5/21/18
pg. 13
Hydrolysis Procedure
Total Fat Calculation
The hydrolysis procedure is done prior to fat extraction. When both hydrolysis and fat extraction are done, the Total
Fat contained within a food or feed sample can be calculated using the following formula:
% Total Fat
= 100 x (W
2
– (W
3
+ (C
1
– C
2
)))
W
1
Where:
W
1
= Original weight of sample
W
2
= Weight of dried sample, filter bag, and Diatomaceous Earth (DE) after hydrolysis
W
3
= Weight of dry extracted sample, filter bag, and DE
(determined after using the ANKOM
XT15
or ANKOM
XT10
instruments)
C
1
= Blank filter bag dry weight after hydrolysis
C
2
= Blank filter bag weight after extraction
Hydrolysis Support Items
The following support items are needed to perform the hydrolysis procedure:
Item
Recommended Product
Electronic Balance with four-place readout
ANKOM #TB Balance Hardware
ANKOM #TBS Balance Software
Filter Bags
ANKOM #XT4
Bag Holder (used for adding sample to an empty filter bag)
ANKOM #101.2
Diatomaceous Earth (DE)
ANKOM #DE1, DE2
Heat Sealer for sealing the filter bags
ANKOM #HS (120V), #HSi (220V)
Solvent Resistant Marker
ANKOM #F08
Multi-bag Holder (used to hold bags in the Hydrolysis Chamber)
ANKOM #H33
Desiccant Pouch for drying
ANKOM #X45
Oven for drying
ANKOM #RD (120V), #RDI (220V)
Sample
Spoon
Содержание XT4
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