Amersham Biosciences Hoefer DALT, Руководство пользователя, Страница: 55 / 70

Hoefer DALT System Gradient Gel Solutions
Amersham Biosciences
51
For 12 gels, 1.0 mm
400 ml
each Heavy and Light,
by volume
in ml.
Light Solution
Heavy Solution
Final %T 7 8 9 10 11 12 13 14 15 16 17 18 19
Acryl. Stock
93 107 120 133 147 160 173 187 200 213 227 24 253
1.5M Tris-Cl,
pH 8.8
100 100 100 100 100 100 100 100 100 100 100 100 100
Water
198 184 171 158 145 131 118 105 92 78 65 52 38
10% SDS
4 4 4 4 4 4 4 4 4 4 4 4 4
Glycerol
0 0 0 0 0 0 0 0 0 0 0 0 0
10% APS
4 4 4 4 4 4 4 4 4 4 4 4 4
10% TEMED
0.98 0.86 0.76 0.68 0.62 0.57 0.53 0.49 0.46 0.43 0.40 0.38 0.36
Final %T 8 9 10 11 12 13 14 15 16 17 18 19 20
Acryl. Stock
107 120 133 147 160 173 187 200 213 227 240 253 267
1.5M Tris-Cl,
pH 8.8
100 100 100 100 100 100 100 100 100 100 100 100 100
Water
160 147 133 120 107 93 80 67 53 40 27 13 0
10% SDS
4 4 4 4 4 4 4 4 4 4 4 4 4
Glycerol
27 27 27 27 27 27 27 27 27 27 27 27 27
10% APS
2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00
10% TEMED
0.28 0.26 0.23 0.21 0.19 0.18 0.16 0.15 0.14 0.14 0.13 0.12 0.11