Amersham Biosciences Hoefer DALT, Руководство пользователя, Страница: 54 / 70

Gradient Gel Solutions Hoefer DALT System
50
Amersham Biosciences
For 25 gels, 1.0 mm
750 ml each Heavy and Light, by volume in ml.
Light Solution
Heavy Solution
Final %T 7 8 9 10 11 12 13 14 15 16 17 18 19
Acryl. Stock
175 200 225 250 275 300 325 350 375 400 425 450 475
1.5M Tris-Cl,
pH 8.8
188 188 188 188 188 188 188 188 188 188 188 188 188
Water
371 346 321 296 271 246 222 197 172 147 122 97 72
10% SDS
7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50
Glycerol
0 0 0 0 0 0 0 0 0 0 0 0 0
10% APS
7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50
10% TEMED
1.84 1.61 1.43 1.28 1.16 11.07 0.99 0.92 0.86 00.80 0.76 0.71 0.68
Final %T 8 9 10 11 12 13 14 15 16 17 18 19 20
Acryl. Stock
200 225 250 275 300 325 350 375 400 425 450 475 500
1.5M Tris-Cl,
pH 8.8
188 188 188 188 188 188 188 188 188 188 188 188 188
Water
300 275 250 225 200 175 150 125 100 75 50 25 0
10% SDS
7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50 7.50
Glycerol
51 51 51 51 51 51 51 51 51 51 51 51 51
10% APS
3.75 3.75 3.75 3.75 3.75 3.75 3.75 3.75 3.75 3.75 3.75 3.75 3.75
10% TEMED
0.53 0.48 0..43 0.39 0.35 0.33 0.31 0..29 0.26 0.26 0.24 0.23 0.21