Amersham Biosciences AKTA design P-960, Руководство пользователя, Страница: 20 / 40

4
Maintenance
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Pump P-960 User Manual 18-1172-73 Edition AA
4.2 General care
Inspect the pump daily for liquid leaks.
If you suspect that air bubbles are trapped in the sample flow path, go
through the air removal procedure for your configuration described in
section 5.2 Removing trapped air bubbles from the sample pump, or in
ÄKTAdesign Optional Configurations User Manual.
4.2.1 General recommendations for all eluents
It is essential that all liquids passing through the pump are clean and
pure. Impure or dirty liquid might block the channels in the pump
assembly, and hence shortening their lifetime. Degassing prevents
formation of air bubbles, which can cause pressure pulsation and
inaccurate flow rate.
4.2.2 Additional recommendations for aqueous liquids
After a run with an aqueous liquid, the pump should always be
thoroughly washed with pure, distilled water to prevent salt
precipitation.
4.3 Cleaning-in-place
Pump a cleaning or sanitizing agent through the pump. The standard
recommendation is to use 1 M NaOH and then wash out immediately
with buffer and distilled water.
WARNING! CORROSIVE CHEMICALS. NaOH is corrosive and
therefore dangerous to health. Avoid spillage and wear protective
glasses.
CAUTION! NaOH might damage the wetted parts in the pump if left
for longer periods. After cleaning using NaOH, flush the pump
immediately with buffer and distilled water.
18117273aa.book Page 20 Thursday, July 3, 2003 5:13 PM