Device User’s Guide
6. Instrument setting for fluorescence measurement
24
6.
Instrument setting for fluorescence measurement
By irradiating and exciting the fluorescent sample with LED light, the irradiated weak
fluorescence spectrum of the sample is detected at a 90 degree angle. In this case, a
strong excitation light is required, so we recommend an optional high power LED light
source.
Application example using high power LED
①
The spectrometer and the light source are set as shown in Figure6-1.
Set the fiber collimator to the cuvette holder and connect it to the spectrometer. And,
then set it to the plataform, and fix with the platform screws. In addition, attach the
collimator lens or the SMA 905 adapter for light source to the cuvette holder optical
path, at a 90 ° position, and connect the LED light source with a optical fiber. Attach the
light shielding adapter to the remaining open optical path sides of the cuvette holder.
Moreover, by preparing a mirror plug for fluorescence measurement in place of the
light shielding adapter, it is possible to obtain greater fluorescence intensity.
②
Turn on the LED light source and adjust the knob of the lish intensity.
③
Open the SpectraSmart software and set to the spectrum mode.
④
Set the solution containing the fluorescent substance in the cuvette holder and
perform the measurement.
Configuration example
PC (sold separately)
SEC2021 Spectrometer
SEC2023 Cuvette holder
Fiber collimator
SMA905 adaptor for light source or Collimating
lens (sold separately)
SMA905 adaptor for light shielding or
Mirror
screw plug (sold separately)
LMS Series High power LED light source (sold
deparately)
600-micron Fiber, solarization-resistant, 25 cm
(sold deparately)
Figure 6-1:
Fluorescence measurement using high power LED light source
