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Table of Contents

1. INTRODUCTION

1.1

About the ELSD 2000 ............................................................................................................................. 4

1.2

What Is Included with the ELSD 2000 .................................................................................................... 4

1.3

Principle of Operation ............................................................................................................................. 5

2. INSTALLATION

2.1

What You Will Need ................................................................................................................................ 6

2.2

Unpacking ............................................................................................................................................... 6

2.3

Controls and Features ............................................................................................................................ 7

2.3.1 Front Panel .................................................................................................................................... 7

2.3.2 Left Side Panel .............................................................................................................................. 7

2.3.3 Right Side Panel ............................................................................................................................ 8

2.3.4 Rear Panel ..................................................................................................................................... 8

2.4

Making Electrical and Fluid Connections ................................................................................................ 9

3. INSTRUMENT CONTROL

3.1

ELSD 2000 Control Options ................................................................................................................. 12

3.2

Using the ELSD 2000 Control Panel .................................................................................................... 12

3.3

Navigating the Screens ......................................................................................................................... 12

3.4

Powering Up ......................................................................................................................................... 13

3.5

Operation Screen .................................................................................................................................. 13

3.6

Instrument Status .................................................................................................................................. 13

3.6.1 Standby ........................................................................................................................................ 13

3.6.2 Run .............................................................................................................................................. 13

3.7

Menu Screen ........................................................................................................................................ 14

3.8

Setting Up a Run .................................................................................................................................. 14

3.9

Method Screen ..................................................................................................................................... 15

3.10 Method Options .................................................................................................................................... 15

3.10.1 Select a Previously Saved Method ............................................................................................ 15

3.10.2 Edit an Existing Method ............................................................................................................. 16

3.10.3 Create a New Method ................................................................................................................ 17

3.11 Configuration Screen ............................................................................................................................ 17

1

Содержание ELSD 2000

Страница 1: ...l 8 2 4 Making Electrical and Fluid Connections 9 3 INSTRUMENT CONTROL 3 1 ELSD 2000 Control Options 12 3 2 Using the ELSD 2000 Control Panel 12 3 3 Navigating the Screens 12 3 4 Powering Up 13 3 5 Op...

Страница 2: ...g Initial Conditions for Impactor On Mode 22 4 5 Start Up Sequence 23 4 6 Shutdown Sequence 24 4 7 Optimization Procedure 24 4 7 1 Optimization for Impactor Off Mode 24 4 7 2 Optimization for Impactor...

Страница 3: ...e Mobile Phase Modifiers 37 6 5 ELSD 2000 Cleaning Procedures 38 6 5 1 Drift Tube Cleaning Procedure 38 6 5 2 Nebulizer Cleaning Procedure 38 6 6 Power Module Adjustments 39 6 6 1 Changing the Input V...

Страница 4: ...adients and also requires that the analyte have a chromophore The ELSD is not plagued by these limitations Unlike these other detectors the ELSD can achieve stable baselines with multisolvent gradient...

Страница 5: ...optical cell for maximum sensitivity This mode is best for analyzing non volatile compounds and or compounds separated using volatile mostly organic mobile phases In the Impactor On mode the aerosol c...

Страница 6: ...chromatographic system Exhaust System A fume hood or other ventilation device located close to the detector to remove the detector exhaust from the laboratory Use only the exhaust trap kit provided w...

Страница 7: ...c functions All instrument parameters for the ELSD 2000 can be displayed and controlled through the front panel 2 Numeric Keypad Membrane based keypad provides values 0 through 9 asterisk and Enter fo...

Страница 8: ...1 through 7 currently have programmed functions CAUTION Operation at the wrong input voltage may damage the detector Refer to Section 6 6 Power Module Adjustments for details on changing the voltage s...

Страница 9: ...the power module on the rear panel of the detector Make sure the unit is set to the proper voltage The voltage is field selectable for operation at 120V US standard or 240V European Standard 3 Gas Con...

Страница 10: ...the detector to the PC or the AllChrom interface box Refer to the appropriate manual for further instructions 6 Exhaust Connection Connect the exhaust elbow provided to the EXHAUST outlet on the rear...

Страница 11: ...tector Consult the appropriate manuals for wiring details NOTE The fault relay override function can be used to temporarily disable the fault relay without disconnecting the wiring Refer to Section 3...

Страница 12: ...ails on PC control of the ELSD 2000 please consult the ELSD 2000 Control Software Manual for instructions 3 2 USING THE ELSD 2000 CONTROL PANEL The ELSD 2000 can be controlled directly from the front...

Страница 13: ...urrent reading of the drift tube temperature in o C Gas Flow Setpoint and current reading of the nebulizer gas flowrate in L min Gain Current gain setting Possible values are 1 2 4 8 and 16 A gain set...

Страница 14: ...ptions for details 2 Configure the instrument Set the following non method dependent parameters Audio Alarm Full Scale Voltage Fault Relay Override Refer to Section 3 12 Configuration Options for deta...

Страница 15: ...1 METHOD B 2 METHOD C 3 METHOD D 4 METHOD E Menu More Confirmation Number 0 Name METHOD A Temperature 70 0 C Gas Flow 1 9 L min Gain 1 Impactor Off OK Cancel From the Method List screen you may selec...

Страница 16: ...the method 2 Method Name Press 2 to change the method name This will bring up the Edit Name screen 3 Tube Temperature Press 3 to change the drift tube temperature and the current setpoint will flash...

Страница 17: ...to Section 3 7 Menu Screen for details on using the Menu screen 3 10 3 CREATE A NEW METHOD Create a new method by saving the new method name number and parameter settings over a previously existing m...

Страница 18: ...f the pump flow This feature is useful when you want to change operating conditions and do not want to trigger the fault relay while the instrument equilibrates In the Off position the fault relay wil...

Страница 19: ...following sections for details NOTE Only the 500mL drain container provided can be positioned at bench level Larger containers must be placed at floor level 2 Mobile phase should not be flowing when...

Страница 20: ...n organic solvents for evaporation Higher mobile phase flowrates also require higher drift tube temperatures than lower mobile phase flowrates Lower gas flowrates produce larger droplets and therefore...

Страница 21: ...operating temperature of the detector is 120o C Solvents that boil above 120o C can only be used at very low flowrates less than 0 5mL min 4 4 SELECTING INITIAL OPERATING CONDITIONS To select initial...

Страница 22: ...n applications as they are for Impactor Off applications Use the recommendations in Table 2 as a starting point regardless of the exact mobile phase composition Refer to Section 4 7 Optimization Proce...

Страница 23: ...eed the recommended maximum solvent flow rates 13 Check connections for leaks and tighten the fittings if necessary 14 Observe the signal output on the display and the recorded baseline After an initi...

Страница 24: ...ments until the baseline noise increases 5 The optimal drift tube temperature will be the lowest temperature that will produce an acceptable low noise baseline NOTE The last method in use before shutd...

Страница 25: ...entifying the optimal gas flowrate NOTE Settings will need to be re optimized when any changes in mobile phase composition or flowrate are made 4 7 2 OPTIMIZATION FOR IMPACTOR ON MODE Impactor On appl...

Страница 26: ...essure pressure is low 40 psi is too low to between 65 80psi Gas source may be low or empty Replace gas source if necessary Possible gas pressure sensor Contact Alltech error Optical sensor or Signal...

Страница 27: ...ser off It offers the following options 1 Diagnostic Tests Used to access the Diagnostic Tests menu 2 Manual Control Used to monitor the temperature of the drift tube and nebulizer zones during equili...

Страница 28: ...ent Max Min Test passed Press any key to continue Current Max Min Gas Pressure 21 2 21 1 21 0 psi 6 If test does not pass results screen will read Test failed and then provide a more detailed error me...

Страница 29: ...ate Any flowrate setting up to 4 0 min can be used Wait several minutes for the unit to equilibrate 3 Press 3 from the Diagnostic Tests screen to start the Flow Meter Test The testing screen will then...

Страница 30: ...efore the for blockage and detector remedy replace as necessary 7 Press any key to return to the Diagnostic Tests screen 5 3 4 COMMUNICATION TEST NOTE The Communication Test only checks the RS 232 wir...

Страница 31: ...le the remaining logs will then be designated Old record s Manual Control 1 Laser On 5 5 mV 2 Gas Flow On 17 9 psi 2 0 L min 3 Drift Tube Heater On 52 0 C 4 Nebulizer Zone Heater On 52 0 C Menu 5 4 MA...

Страница 32: ...or drift tube and cleaning procedures Contact Alltech for details on cleaning optics 1 The detector flow cell and or laser optics may need cleaning Contact Alltech for details 2 Check data cable for n...

Страница 33: ...oo low gas flowrate following the optimization procedure in Section 4 7 Gas source contaminated or of low purity Use clean dry inert gas usually 99 9 pure nitrogen Mobile phase contaminated or made of...

Страница 34: ...logged inlet gas filter Replace filter Contact Alltech Gas source may be low or empty Check gas source Replace if needed No power Line unplugged Plug in power line Blown fuse Replace fuse Refer to Sec...

Страница 35: ...n Section 6 5 for cleaning procedures sensitivity Detector settings changed Check drift tube temperature gas flow and impactor settings Autosampler needle not pulling up sample Repair or replace equip...

Страница 36: ...1 Laser 600509 1 O Ring for Nebulizer 600514 1 Nebulizer 600805A 1 ELSD 2000 Control CD ROM 272106 1 RS 232 Cable for ELSD 2000 600101M 1 ELSD 2000 PC Control Operating Manual 6 APPENDIX 6 1 SPECIFICA...

Страница 37: ...6 C Ethylamine 10 66 3 34 6 3 C Triethylamine 11 01 2 99 89 3 C Buffers Ammonium Formate 3 0 5 0 120 C Pyridinium Formate 3 0 5 0 Ammonium Acetate 3 8 5 8 111 C Pyridinium Acetate 4 0 6 0 Ammonium Ca...

Страница 38: ...g procedure Phillips head screwdriver Hex Ball driver supplied with ELSD accessory kit 1 4 standard wrench Sonicator bath HPLC grade Water 50 50 Methanol Water mixture HPLC grade solvents 2 Switch the...

Страница 39: ...1 CHANGING THE INPUT VOLTAGE 1 Power off the detector and unplug the power cord from the rear panel of the detector 2 Insert the blade of a small screwdriver into the slot next to the power connector...

Страница 40: ...in the warranty period and or parts which are subjected to effects of corrosion or deterioration by chemical or other action are excluded from the above warranty for malfunction because of inadequate...

Страница 41: ...esponse of a Light Scattering Detector Michel Righezza Journal of Liquid Chromatography 11 9 10 1967 2004 1988 Effects of Wavelength of the Laser Beam on the Response of an Evaporative Light Scatterin...

Страница 42: ...Chromatography 18 97 100 1995 Analysis of Lipoxygenase Kinetics by High Performance Liquid Chromatography with a Polymer Column A Nunez and G J Piazza Lipids 30 2 129 133 1995 Gradient Reversed Phase...

Страница 43: ...phy 459 91 107 1988 Chemical Characterization of Cellulose Acetate by Non Exclusion Liquid Chromatography T R Floyd Journal of Chromatography 629 243 254 1993 Separation of Neutral Lipids by High Perf...

Страница 44: ...matographic Science 30 497 499 December 1992 Newer Method for the Characterization of Higher Molecular Mass Coal Derivatives Keith D Bartle Erdol und Kohle Erdgas Petrocheme Vereinigt mit Brennstoff 3...

Страница 45: ...April 2001 Manual Part No 600100M ALLTECH ELSD 2000 EVAPORATIVE LIGHT SCATTERING DETECTOR OPERATING MANUAL...

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