Agilent Technologies Magnis NGS Prep System Скачать руководство пользователя страница 10

Running Protocols

10

Magnis NGS Prep System Quick Reference Guide

Run a quick

cycle

Agilent recommends running the decontamination quick cycle before each protocol run.

1

Verify that the instrument deck is cleared of all labware, then make sure that the instrument 
door is closed.

2

From the Home screen, press 

Decontamination

.

The Decontamination screen opens.

Figure 5

Decontamination screen with Quick cycle selected

3

At the top of the screen, select 

Quick cycle

, as shown in 

Figure 5

4

Press 

Start

.

The decontamination cycle begins and the screen displays a countdown of the time remaining.
At the completion of the cycle, the UV light turns off and the instrument remains idle.
If needed, press 

Abort

 at any time to stop the decontamination cycle.

Running Protocols

1) System prep

Prepare the Magnis NGS Prep System so that it is ready to run a protocol. 

1

Verify that the instrument deck is cleared of all labware from previous runs.

2

Turn on the instrument and close the instrument door.
See 

“Turn on the instrument” 

on page 7 for instructions.

3

At the Login screen, enter the credentials for your user account.
Each time the system is turned on, it performs a series of start up activities (see 

Table 3

 on 

page 8) immediately after login. These activities may take several minutes to perform. Make 
sure the instrument door remains closed for the duration of these activities. After completion 
of the start up activities, the software opens to the Home screen and the system’s LED light is 
blue, indicating that it is ready for use.

4

(Optional) Perform the 30-minute UV decontamination quick cycle to decontaminate the 
surfaces of the instrument deck. See 

“Decontaminating the System” 

on page 9.

You can begin 

“2) Reagent prep”

 while the decontamination is running.

Содержание Magnis NGS Prep System

Страница 1: ...7 Log in to the system 7 Creating User Accounts 8 About user access levels 8 Add new user accounts 9 Decontaminating the System 9 Running Protocols 10 1 System prep 10 2 Reagent prep 11 3 Protocol se...

Страница 2: ...tem is designed for safe operation when it is used in the intended manner Using the system for purposes other than those intended may impair those safety protections Installation The system must be in...

Страница 3: ...not service the electrical components unless you are qualified to do so Fluids and Reagents Never process reagents not intended for use with the Magnis NGS Prep System Observe the relevant safety reg...

Страница 4: ...rument in high static environments ESD STM5 1 1998 Class 3B Environmental Requirements Operating temperature Keep the ambient temperature between 15 C and 25 C Operating humidity Keep the humidity lev...

Страница 5: ...eps Chiller module for reagent storage Tube holders for liquid reagents 6 total Platforms to hold tip boxes 4 Platform to hold Beads Buffers Plate Opening to tip waste bin LED indicator lights 2 Micro...

Страница 6: ...status of the instrument based on the color of the LED indicator lights that illuminate the entire plate filling area Micropipettor Barcode scanner UV tube Instrument deck LED indicator light LED indi...

Страница 7: ...the user name and password for your account into the provided fields The Agilent engineer or Agilent authorized service provider creates a user account during system installation that has advanced us...

Страница 8: ...of markers called teach points that are printed on the instrument deck Auto teaching ensures that during a run the micropipettor is accurately aligned with the tubes or wells at each deck position You...

Страница 9: ...s to send an email alert to the user it sends it to all email addresses listed in the Email address es field 8 Mark the check box labeled Email alert on run complete if you want the system to send an...

Страница 10: ...prep Prepare the Magnis NGS Prep System so that it is ready to run a protocol 1 Verify that the instrument deck is cleared of all labware from previous runs 2 Turn on the instrument and close the inst...

Страница 11: ...orms an IHC At completion of the IHC the Enter Run Info step opens 2 Complete the Run Setup steps and start the protocol run as instructed in the user manual for your Magnis Target Enrichment Kit The...

Страница 12: ...llect the final library samples i e the green library strip tube from the chiller module 3 Re seal the wells of the strip tube using a fresh foil seal strip then store at the temperature recommended i...

Страница 13: ...7 9770 option 3 4 4 Or send an email to techservices agilent com For Europe Middle East Africa and India Call 00800 345 600 toll free or 49 69 8679 7730 Or send an email to genomics_tech_europe agilen...

Отзывы: