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Agilent Technologies G7129A Скачать руководство пользователя страница 172

Agilent InfinityLab LC Series Vialsamplers User Manual

172

5

Optimizing Performance

Fast Injection Cycle and Low Delay Volume

General Recommendations for Fast Injection Cycle

Times

As described in this section, the first step to provide short cycle times are
optimizing the chromatographic conditions. If this is done the autosampler
parameter should be set to:

•

Overlapped injection mode

•

Increase of draw and eject speed for large injection volumes

•

Add at last run a blank, if overlapped injection is used

To reduce the injection time, the detector balance has to be set to OFF.

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Содержание G7129A

Страница 1: ...Vialsamplers Agilent InfinityLab LC Series User Manual Agilent InfinityLab LC Series Vialsamplers User Manual...

Страница 2: ...ng the material in this document that conflict with these terms the warranty terms in the separate agreement shall control Technology Licenses The hardware and or software described in this document a...

Страница 3: ...and performance specifications 3 Using the Module This chapter provides information on how to use the module 4 Preparing the Module This chapter explains the operational parameters of the module 5 Op...

Страница 4: ...ance 10 Identifying Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules 11 Hardware Information This chapter describes the detector in more detail on...

Страница 5: ...fications 38 Performance Specifications 39 Specifications of the Sample Cooler 47 Specifications of the Sample Thermostat 49 Specifications of the Integrated Column Compartment 51 3 Using the Module 5...

Страница 6: ...for Lowest Carryover 167 Fast Injection Cycle and Low Delay Volume 171 Precise Injection Volume 173 Choice of Rotor Seal 175 6 Troubleshooting and Diagnostics 176 Status Indicators 177 Overview of Tes...

Страница 7: ...ic Pump Cartridge 261 Exchange the Wash Port Assembly 263 Replace the Module Firmware 269 Replace the Sample Cooler Sample Thermostat 270 9 Parts and Materials for Maintenance 275 Standard Parts For M...

Страница 8: ...Layout 330 Early Maintenance Feedback EMF 331 12 LAN Configuration 332 What You Have to Do First 333 TCP IP parameter configuration 334 Configuration Switches 335 Initialization Mode Selection 336 Dy...

Страница 9: ...Features G7129B 13 Product Description G7129C 14 Features G7129C 15 Overview of the Module 16 Operating Principle 18 Sampling Sequence 18 Needle Parkstation 23 Hydraulic Box 24 Transport Assembly 26...

Страница 10: ...e needed for routine pharmaceutical tasks and quality control as well as for environmental and food analyses It can house optionally the integrated column compartment for two LC columns with temperatu...

Страница 11: ...temperature control with an integrated column compartment as option or upgrade available Which holds two columns up to 30 cm length and provides heating capacity from 5 C above ambient up to 80 C for...

Страница 12: ...iability safety and ease of use needed for routine pharmaceutical tasks and quality control as well as for environmental and food analyses It can house optionally the integrated column compartment for...

Страница 13: ...ure control with an integrated column compartment as option or upgrade available Which holds two columns up to 30 cm length and provides heating capacity from 5 C above ambient up to 80 C for reproduc...

Страница 14: ...e needed for routine pharmaceutical tasks and quality control as well as for environmental and food analyses It can house optionally the integrated column compartment for two LC columns with temperatu...

Страница 15: ...temperature control with an integrated column compartment as option or upgrade available Which holds two columns up to 30 cm length and provides heating capacity from 5 C above ambient up to 80 C for...

Страница 16: ...older Agilent samplers The Vialsampler can also be equipped with an external tray which can be beneficial for automation The Agilent WalkUp solution fully supports the external tray The Vialsampler fe...

Страница 17: ...n the LC stack It has the heating capability of reaching temperatures up to 80 C and can hold a total of two columns with maximum lengths of 30 cm For applications requiring control over the vial temp...

Страница 18: ...ss main path position at the end of the sampling sequence The sampling sequence occurs in the following order 1 The injection valve switches to the bypass position 2 The piston of the metering device...

Страница 19: ...t of the injection sequence and during an analysis the injection valve is in the mainpass main path position see Figure 4 on page 19 In this position the mobile phase flows through the autosamplers me...

Страница 20: ...he valve unit switches to the bypass position see Figure 5 on page 20 Solvent from the pump enters the valve unit at port 1 and flows directly to the column through port 6 Figure 5 Valve in bypass nee...

Страница 21: ...e needle The needle moves down into the vial the metering unit draws the required sample volume into the loop and the needle is raised In the next step the needle is washed see Figure 6 on page 21 Fig...

Страница 22: ...n the sample tray The wash port flips into the origin position the needle is lowered into the needle seat and the injection valve switches back to mainpass main path position flushing the sample onto...

Страница 23: ...rive The needle movement is driven by a stepper motor connected to the spindle assembly by a toothed belt The circular motion of the motor is converted to linear motion by the drive nut on the spindle...

Страница 24: ...24 1 Introduction Operating Principle Hydraulic Box The hydraulic box comprises two main assemblies metering device and injection valve Figure 9 Hydraulic Unit NOTE The replacement hydraulic box excl...

Страница 25: ...ring guides the movement of the piston in the analytical head The home position of the piston is sensed by an optical sensor on the hydraulic unit board while the sample volume is determined by count...

Страница 26: ...pening and closing of the gripper fingers are driven by a stepper motor linked by a toothed belt to the planetary gearing inside the gripper assembly The stepper motor positions are determined by the...

Страница 27: ...leak plane outlets are situated in a consistent position so that all Infinity and Infinity II modules can be stacked on top of each other Waste tubes are guided through a channel on the right hand si...

Страница 28: ...Agilent InfinityLab LC Series Vialsamplers User Manual 28 1 Introduction Leak and Waste Handling Figure 11 Infinity II Leak Waste Concept Flex Bench installation...

Страница 29: ...Agilent InfinityLab LC Series Vialsamplers User Manual 29 1 Introduction Leak and Waste Handling Figure 12 Infinity II Single Stack Leak Waste Concept bench installation...

Страница 30: ...nual 30 1 Introduction Leak and Waste Handling Figure 13 Infinity II Two Stack Leak Waste Concept bench installation The waste tube connected to the leak plane outlet on each of the bottom instruments...

Страница 31: ...of the leak sensor The material of the leak sensor PVDF polyvinylidene fluoride is incompatible with DMF Do not use DMF as mobile phase Check the leak sensor regularly for corrosion NOTE The leak sen...

Страница 32: ...ion Leak and Waste Handling Waste Concept 1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports 5043 1221 for optimal and safe waste disposal If you decide to use your ow...

Страница 33: ...ications 39 Performance Specifications G7129A 39 Performance Specifications G7129B 42 Performance Specifications G7129C 45 Specifications of the Sample Cooler 47 Specifications of the Sample Thermosta...

Страница 34: ...n result if the devices are connected to a line voltage higher than specified Connect your instrument to the specified line voltage only WARNING Electrical shock hazard The module is partially energiz...

Страница 35: ...purposes can lead to personal injury or damage of electronic equipment Never use a power cord other than the one that Agilent shipped with this instrument Never use the power cords that Agilent Techno...

Страница 36: ...ple Thermostat is installed Use a bubble level to check the leveling of the sampler WARNING Flammable refrigerant Formation of flammable gas air mixtures inside the Sample Thermostat and laboratory Ke...

Страница 37: ...ng heavy objects Ensure that the load is as close to your body as possible Ensure that you can cope with the weight of your load CAUTION Condensation within the module Condensation can damage the syst...

Страница 38: ...1195 BTU h Ambient operating tempera ture 4 40 C 39 104 F without sample ther mostat up to 55 C 131 F Ambient non operating tem perature 40 70 C 40 158 F Humidity 95 r h at 40 C 104 F 1 1 If a sample...

Страница 39: ...ts with 100 L up to 60 MPa 0 1 900 L in 0 1 L incre ments with 900 L up to 40 MPa Up to 1800 L with multiple draw hardware modification required Requires 900 L analytical head Injection precision 0 25...

Страница 40: ...in Ejection speed 200 L min Injection volume 1 L Minimum sample vol ume 1 L from 5 L sample in 100 L microvial or 1 L from 10 L sample in 300 L microvial Needle height offset has to be adapted to ensu...

Страница 41: ...oftware Leak detection safe leak han dling leak output signal for shutdown of pumping system and low voltages in major main tenance areas GLP features Early maintenance feedback EMF for continuous tra...

Страница 42: ...L incre ments with 1290 Infinity large volume injection kit hardware modification required 0 1 100 L in 0 1 L if 100 L loop and 100 L head is installed Up to 1500 L with 1400 L multi draw kit and 100...

Страница 43: ...in Ejection speed 200 L min Injection volume 1 L Minimum sample vol ume 1 L from 5 L sample in 100 L microvial or 1 L from 10 L sample in 300 L microvial Needle height offset has to be adapted to ensu...

Страница 44: ...oftware Leak detection safe leak han dling leak output signal for shutdown of pumping system and low voltages in major main tenance areas GLP features Early maintenance feedback EMF for continuous tra...

Страница 45: ...11603 psi Sample viscosity range 0 2 5 0 cP Sample capacity 132 x 2 mL vial two trays default 100 x 2 mL vial two classic trays optional 36 x 6 mL vials two trays optional Carryover 0 004 40 ppm with...

Страница 46: ...ove For details about supported software versions refer to the compatibility matrix of your ver sion of the LC CE Drivers Communication Controller Area Network CAN Local Area Network LAN ERI ready sta...

Страница 47: ...bel Table 5 Physical Specification of the Sample Cooler Type Specification Comment Weight 6 kg 13 2 lbs Dimensions height width depth 205 x 340 x 370 mm 8 1 x 13 4 x 14 6 inches Refrigerant gas HFC 13...

Страница 48: ...ct your local Agilent Service Center in regard to safe environmental disposal of the appliance or check www agilent com for more info Table 6 Performance Specifications of the Sample Cooler Type Speci...

Страница 49: ...arnings listed in the manual Table 7 Physical Specifications of the Sample Thermostat Type Specification Comments Weight 6 kg 13 2 lbs Dimensions height x width x depth 205 x 340 x 370 mm 8 1 x 13 4 x...

Страница 50: ...lations Please contact your local Agilent Service Center in regard to safe environmental disposal of the appliance or check www agilent com for more info Table 8 Performance Specifications for the Sam...

Страница 51: ...x 396 0 x 106 5 mm 3 4 x 15 6 x 4 2 inches Maximum outside Supply Voltage 24 V DC Power consumption 110 W Ambient operating temperature 4 55 C 39 131 F Ambient non operating tem perature 40 70 C 40 15...

Страница 52: ...ations Agilent InfinityLab LC Series Integrated Column Compartment G7130A Table 10 Performance Specifications G7130A Type Specification Comments Temperature range 5 C above ambient to 80 C Column capa...

Страница 53: ...mn Compartment 76 Install a Column in the ICC 82 Remove a Column from the ICC 86 Install the Column Shelf 88 Install the Column ID Upgrade Kit 92 Connect a Column Identification Tag to the Tag Reader...

Страница 54: ...mple Temperature 129 Operation Information 130 Transporting the Sampler 132 Prepare a Sampler Without Cooler Thermostat for Transportation 132 Prepare a Sampler with Cooler Thermostat for Transportati...

Страница 55: ...Agilent InfinityLab LC Series Vialsamplers User Manual 55 3 Using the Module Magnets Magnets 1 Magnets in doors of pumps autosamplers detectors and fraction collectors...

Страница 56: ...Agilent InfinityLab LC Series Vialsamplers User Manual 56 3 Using the Module Turn on off Turn on off This procedure exemplarily shows an arbitrary LC stack configuration 1 2 Power switch On...

Страница 57: ...Agilent InfinityLab LC Series Vialsamplers User Manual 57 3 Using the Module Turn on off 3 Turn instrument On Off with the control software 4 Power switch Off 5...

Страница 58: ...ix possible module conditions Status indicators 1 Idle 2 Run mode 3 Not ready Waiting for a specific pre run condition to be reached or completed 4 Error mode interrupts the analysis and requires atte...

Страница 59: ...n using two drawers the numbering convention is slightly different The vial positions of the right hand drawer begin at position P2 A1 as follows Left hand Drawer for 66 x 2 mL Vials P1 A1 to P1 F11 L...

Страница 60: ...ernal tray by turning and pushing it into the backside of the hole position No 206 Figure 14 Numbering of drawer position left hand Drawer for 66 x 2 mL Vials Figure 15 Numbering of drawer position ri...

Страница 61: ...lers User Manual 61 3 Using the Module Vial Drawers and Trays Figure 18 Numbering of drawer position Drawer for 50 x 2 mL Vials Classic Figure 19 Numbering of tray position External tray 1 50 51 100 2...

Страница 62: ...Vial Drawers and Trays Exchange Drawers NOTE Do not operate the sampler without drawers installed NOTE Install all drawers for best cooling performance NOTE Do not mix standard and classic drawers Ins...

Страница 63: ...Series Vialsamplers User Manual 63 3 Using the Module Vial Drawers and Trays 1 Open the doors of the module 2 Remove the drawer a Pull the drawer out b Lift the front of the drawer c Lift the drawer o...

Страница 64: ...nt InfinityLab LC Series Vialsamplers User Manual 64 3 Using the Module Vial Drawers and Trays 3 Install the drawer a Insert the back of the drawer b Align the drawer c Push in the drawer 4 Close the...

Страница 65: ...into the sampler while the gripper arm is moving might lead to aborting the ongoing analysis 1 Open the doors of the module NOTE For accessing the left drawer it is sufficient to open the left door on...

Страница 66: ...Agilent InfinityLab LC Series Vialsamplers User Manual 66 3 Using the Module Vial Drawers and Trays 5 Close the doors...

Страница 67: ...er Parts required p n Description G7129 60000 External Tray for 5 x 2 mL Vials G1313 27302 Disposal tube Preparations Turn off the sampler Open the doors Remove the samples NOTE Keep foam and plastic...

Страница 68: ...lastic cover a Lift up the front part of the foam with a flathead screw driver b Remove the foam c Push out the plastic cover 2 Install the external tray and the disposal tube a Mount the external tra...

Страница 69: ...the Module Vial Drawers and Trays 3 Close the doors 4 Configure the External Tray in the online view of the Chromatographic Data System right click on the sam pler dashboard and select Modify Externa...

Страница 70: ...m of 15 000 injections with the autosampler Crimp Top Vials p n Description 5181 3375 Crimp Top Vial 2 mL clear glass 100 Pack 5183 4491 Crimp Top Vial 2 mL clear glass 1000 Pack 5182 0543 Crimp Top V...

Страница 71: ...write on spot 100 Pack 5183 4506 Snap Top Vial 2 mL amber glass write on spot 1000 Pack 5183 4509 Snap Top Vial 2 mL amber glass write on spot 100 Pack silanized p n Description 5182 0714 Screw Cap Vi...

Страница 72: ...r polypropylene septum clear PTFE red rubber 100 Pack 5182 3458 Snap Cap blue polypropylene septum clear PTFE red rubber 100 Pack 5182 3457 Snap Cap green polypropylene septum clear PTFE red rubber 10...

Страница 73: ...ld never exceed 40 mm p n Description 9301 1377 Screw Top Vial 6 mL clear glass flat bottom 100 Pack 5188 5369 Screw Top Vial 5 mL clear glass high recovery 100 Pack 9301 1379 Screw Cap 16 mm w o sept...

Страница 74: ...al problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged Notify your Agilent...

Страница 75: ...ivery checklist is shown below For parts identification please check the illustrated parts breakdown in Integrated Column Compartment on page 288 Please report any missing or damaged parts to your loc...

Страница 76: ...ed p n Description 1 G7130 60030 Integrated Column Compartment 3 L Heater OR 1 G7130 60060 Integrated Column Compartment 6 L Heater 1 Accessory Kit for ICC includes capillaries and column holder clips...

Страница 77: ...lumn shelf a Press in the release buttons on the left and right side b Pull out the column shelf The column shelf is removed 2 Install the Integrated Column Compartment a Push in the Integrated Column...

Страница 78: ...ompartment to the left 4 Open the flap door 5 Connect the injector valve port 6 of the Vialsampler to the inlet port of the ICC heat exchanger with a suitable capillary see Capillary Connections on pa...

Страница 79: ...Column Compartment 7 Tighten the capillary fitting to make the connection pressure tight NOTE Do not overtighten the fitting maximum torque 2 5 Nm NOTE The use of capillaries with SL or SX fittings a...

Страница 80: ...the column onto the Quick Connect Fitting until the first resistance is observed b Close the lever to establish a pressure tight connec tion 10 Connect a suitable capillary to the outlet port of the c...

Страница 81: ...12 Route the capillary coming from the column through the opening between the flap door and the ICC body towards the detector module NOTE Alternatively the capillary can also be routed through the in...

Страница 82: ...pillary Connections on page 278 Tools required p n Description 8710 0510 Open end wrench 1 4 5 16 inch Parts required p n Description Column up to 30 cm length G7116 68003 Column Holder Lamella 2 pk P...

Страница 83: ...ctions on page 278 for recommendations 4 Connect a suitable capillary to the respective outlet port of the ICC heat exchanger see Capillary Connections on page 278 for recommendations 5 Tighten the ca...

Страница 84: ...the column onto the Quick Connect Fitting until the first resistance is observed b Close the lever to establish a pressure tight connec tion 8 Connect a suitable capillary to the outlet port of the co...

Страница 85: ...CC body towards the detector module NOTE Alternatively the capillary can also be routed through the incisions at the right side of the ICC dedicated for this pur pose 11 Close the flap door until the...

Страница 86: ...d p n Description 8710 0510 Open end wrench 1 4 5 16 inch Preparations Switch off the pump Switch off the sampler Open the doors of the sampler 1 Push the latch of the Integrated Column Compartment to...

Страница 87: ...l Integrated Column Compartment 5 Remove the capillary from the outlet port of the column 6 Lift up the lever to release the tension on the Quick Con nect Fitting 7 Unscrew and remove the Quick Connec...

Страница 88: ...reparations Switch off the pump Switch off the sampler Open the doors of the sampler Open the ICC flap door WARNING Fire or explosion due to missing leak drainage Solvents may run into the module if c...

Страница 89: ...Quick Turn Fitting either with your fingers or with the help of two wrenches 4 Remove the capillary from the outlet port of the column 5 Lift up the lever to release the tension on the Quick Con nect...

Страница 90: ...ntegrated Column Compartment a Press the release buttons on the left and right side b Pull out the Integrated Column Compartment The Integrated Column Comparment is removed 10 Install the column shelf...

Страница 91: ...Agilent InfinityLab LC Series Vialsamplers User Manual 91 3 Using the Module Install the Optional Integrated Column Compartment 11 Close the doors 12 Turn on the sampler and the pump...

Страница 92: ...ication Tag Preparations Power off the instrument Install the Integrated Column Compartment see Install the Integrated Column Compartment on page 76 NOTE To use the G4751A Column ID Upgrade Kit the Ag...

Страница 93: ...ng the Module Install the Optional Integrated Column Compartment Next Steps 5 Power on the instrument 6 Place a column with an identification tag in the ICC and plug the tag into the tag reader see Co...

Страница 94: ...up to 30 cm length Preparations Install the Column ID Tag Reader see Install the Column ID Upgrade Kit on page 92 Remove the column if installed see Remove a Column from the ICC on page 86 1 If the c...

Страница 95: ...e the cord to the left and plug the column identifi cation tag into the corresponding socket of the tag reader 4 Close the flap door until the latch is locked 5 Close the doors 6 Configure the connect...

Страница 96: ...d When the Integrated Column Compartment ICC is installed in a Vialsampler and configured in the chromatography data system CDS a new tile appears on the dashboard comprising a standalone graphical us...

Страница 97: ...7 3 Using the Module Using the Optional Integrated Column Compartment Control Interface Right clicking on the sampler GUI opens the control interface where control and method parameters can be edited...

Страница 98: ...change some control specific settings for the ICC Thermostat On The ICC turns on and the system starts to regulate the temperature of the column oven towards the setpoint Off The ICC turns off At Pow...

Страница 99: ...ing used for the given method Enforce column for run Marking this checkbox restricts the execution of the method to a specific column type identified by its product number Stoptime As Pump Injector Th...

Страница 100: ...rature The analysis starts regardless of the actual column oven temperature When temperature is within The analysis starts only when the actual column oven temperature is within the specified range of...

Страница 101: ...ional Integrated Column Compartment Online Signals In the Online Signals tab of the CDS the actual temperature of the column oven can be configured and plotted together with the other instrument actua...

Страница 102: ...section Column Tag Information This section holds information on the physical properties of the columns installed in the ICC and their usage see Column Tag Information Table on page 103 for more info...

Страница 103: ...Using the Module Using the Optional Integrated Column Compartment Column Tag Information Table The Column Tag Information table is part of the Column Assignment dialog box which can be prompted by cl...

Страница 104: ...performed with the column These fields are read only from the perspective of the user meaning that their content cannot be manually edited or erased For pre labeled Agilent columns the static fields...

Страница 105: ...h ChemStation on page 105 for more information and the Column Assignment tab of the control interface see Column Assignment on page 102 for more information Using Column ID Tags with ChemStation 1 In...

Страница 106: ...Click the Edit button to display a dialog box that allows you to edit the column specific information such as Serial Number Batch Number and Description 5 Add or edit the other column specific informa...

Страница 107: ...r each valve position 2 The Visualization section gives a visual representation of the Multicolumn Thermostat configuration 3 The Column Tag Information table shows the information stored on the colum...

Страница 108: ...can edit the information on the column ID tag using the ChemStation Edit Columns table When you have finished editing the information repeat steps 7 to 9 to update the information in the tag NOTE It m...

Страница 109: ...ion table and select Seal Column Tag from the context menu The sealed column is shown in the Column Tag Information table with the icon at the beginning of the row In the Edit Columns table of the Che...

Страница 110: ...ment Dashboard panel in the ChemStation s Method and Control view Figure 24 Column Assignment dialog box overview The Column Assignment dialog box has three sections that give you information about th...

Страница 111: ...formation table The Column Tag Information shows the information in the column ID tags for all columns in the configuration including their location in the column compartment and their color codes The...

Страница 112: ...tional columns The table includes not only the columns that are installed in the column thermostat device s but also the inventory of other available columns for example columns that have been used in...

Страница 113: ...right of the table to show the full table Tag Status shows the status of the column ID tag Empty The position is empty or has a column without a column ID tag A column with a column ID tag is install...

Страница 114: ...r The product number of the column Static Read Write Maximum Measured Temperature The highest temperature in C experienced by the column to date Dynamic Read Read Maximum Tempera ture The safe maximum...

Страница 115: ...Column Compartment Tag Sealed Whether or not all static fields except Comment and Void Volume are set irrevocably to read only Static Read Write Comment A user generated comment about the column Stati...

Страница 116: ...le Thermostat Upgrade on page 287 Please report any missing or damaged parts to your local Agilent Technologies sales and service office CAUTION Defective on arrival problems If there are signs of dam...

Страница 117: ...installed in the stack WARNING Flammable refrigerant Formation of flammable gas air mixtures inside the Sample Thermostat and laboratory Keep open fire or sources of ignition away from the device Ens...

Страница 118: ...Cooler Sample Thermostat is disconnected from the power supply wait for at least five minutes before replugging and switching on the compressor again NOTE Even under average humidity conditions a sig...

Страница 119: ...tall the Optional Sample Cooler Sample Thermostat 1 Ensure that the power switch on the front of the module is OFF switch stands out 2 Disconnect the power cable from the sampler 3 Loosen the four scr...

Страница 120: ...Thermostat if it is not operating correctly or has been damaged Dis connect it from the power supply and call your local service center Remove the power cable from the module before open ing the cove...

Страница 121: ...ampler 7 Slide the Sample Cooler Sample Thermostat all the way into the sampler 8 Fix the Sample Cooler Sample Thermostat with the four screws 9 Use a bubble level to check the leveling of the sampler...

Страница 122: ...ctions may hinder the drainage 12 Ensure that the tubing runs straight into the waste con tainer without any bends or joints and that it is free end does not immerse in the liquid NOTE For more inform...

Страница 123: ...Sample Thermostat is incorporated in the graphical user interface GUI of the hosting sampler which appears automatically when the unit is configured in the chromatography data system CDS When the cool...

Страница 124: ...uration modified and special commands executed NOTE The actual temperature may deviate from the set temperature by up to 3 C depending on the temperature setting and ambient conditions NOTE If the act...

Страница 125: ...urns on and the system starts to regulate the temperature inside the sample space towards the setpoint Off The cooler thermostat turns off Enable Analysis With any temperature The analysis starts rega...

Страница 126: ...Agilent InfinityLab LC Series Vialsamplers User Manual 126 3 Using the Module Using the Optional Sample Cooler Sample Thermostat...

Страница 127: ...setting is part of the method parameters The temperature can change from method to method within a given sequence This control mode is not recommended for most analytical workflows but might be used f...

Страница 128: ...nal Sample Cooler Sample Thermostat Online Signals In the Online Signals tab of the CDS the actual temperature of the sample space can be configured and plotted together with the other instrument actu...

Страница 129: ...ure can be included in the analysis report For this the Samples Advanced Run Information field must be included in the report template Figure 26 Finding the Advanced Run Information setting in OpenLab...

Страница 130: ...ormation on the internal surfaces of the sampler This is normal and should cease after a couple of hours at the most NOTE Reaching the 4 C setpoint from an ambient temperature of 22 C takes about 45 m...

Страница 131: ...er thermostat and wait until it defrosts Shutting Down When the Sample Cooler Sample Thermostat needs to be turned off for the night or a longer period the following best practices are recommended Rem...

Страница 132: ...ff the sampler 4 Install the Transport Protection Foam see Install the Transport Protection Foam on page 135 When The module needs to be transported or relocated WARNING Heavy weight The module is hea...

Страница 133: ...Ensure that you can cope with the weight of your load CAUTION Condensate inside the cooler or thermostat Damage to the electronics Unplug the power cords Drain off all condensate before dismounting th...

Страница 134: ...te the drainage of the condensate from the system 3 Remove all vials from the vial drawers 4 Move the transport assembly to the park position using Instant Pilot or Lab Advisor see Park Arm on page 18...

Страница 135: ...orting or shipping the sampler Parts required p n Description G7129 40050 Transport Protection Foam Preparations All vials are removed from the vial drawers 1 Open the doors of the module 2 Move the g...

Страница 136: ...Agilent InfinityLab LC Series Vialsamplers User Manual 136 3 Using the Module Transporting the Sampler 5 Ensure that the foam is snapped behind the upper part of the metal frame 6 Close the doors...

Страница 137: ...ring of Agilent Infinity II Prime LC Modules Excellent usability and ease of use through a user interface specifically tailored for mobile devices simple intuitive touch enabled and visual controllabl...

Страница 138: ...e 1200 Series 1200 Series SL and 1100 Series Excellent readability and usability by large colored display with background light high resolution and contrast Convenient ergonomic operation either handh...

Страница 139: ...le 141 Solvent Information 142 Capillary Color Coding Guide 149 Installing Capillaries 150 Flow Connections to the Vialsampler 152 Setting up the Vialsampler 154 Control Settings 158 Method Parameter...

Страница 140: ...ludes the solvent temperature in the sample compartment For the solvents methanol and ethanol keep the solvent temperature at least 25 K below the boiling point Do not operate the instrument in an exp...

Страница 141: ...vents see Solvent Information on page 142 Priming and Purging the System When the solvents have been exchanged or the system has been turned off for a certain time for example overnight oxygen will re...

Страница 142: ...ilter solvents through 0 22 m filters Avoid or minimize the use of solvents that may corrode parts in the flow path Consider specifications for the pH range given for different materials such as flow...

Страница 143: ...and PharMed tubing to different needle wash solvents Table 14 Solvent Compatibility of Silicone and PharMed Tubing Silicone PharMed Acids weak medium strong good unsatisfactory not recommended very g...

Страница 144: ...available refers to room temperature typically 20 25 C 68 77 F If corrosion is possible it usually accelerates at higher temperatures If in doubt please consult technical literature on chemical compat...

Страница 145: ...vents used in chromatographic systems like methanol acetonitrile and isopropanol It has limited stability with aliphatic aromatic and halogenated hydrocarbons THF phenol and derivatives concentrated a...

Страница 146: ...is highly resistant to oxidizing acids for example nitric perchloric and hypochlorous acid over a wide range of concentrations and temperatures This is due to a thin oxide layer on the surface which i...

Страница 147: ...tinum Iridium is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Fluorinated polymers PTFE PFA FEP FFKM PVDF Fluorinated polymers li...

Страница 148: ...4 Preparing the Module Solvent Information Sapphire Ruby and Al2O3 based ceramics Sapphire ruby and ceramics based on aluminum oxide Al2O3 are inert to almost all common acids bases and solvents There...

Страница 149: ...Agilent InfinityLab LC Series Vialsamplers User Manual 149 4 Preparing the Module Capillary Color Coding Guide Capillary Color Coding Guide Figure 28 Syntax for capillary description...

Страница 150: ...s mentioned above are examples only Parts required p n Description 5067 4650 Capillary ST 0 12 mm x 150 mm SL SX 5067 4651 Capillary ST 0 12 mm x 280 mm SL SX 5067 4720 Capillary ST 0 17 mm x 150 mm S...

Страница 151: ...fit ting which forces the ferrule to seat onto the tubing or capillary NOTE Don t overtighten Overtightening will shorten the lifetime of the fitting 5 Loosen the nut and verify that the ferrule is c...

Страница 152: ...er 5 Prime the wash tubing 6 Install the capillary from the pump outlet into the port 1 of the injection valve Preparations Module is installed in the system Use an appropriate solvent based on the sa...

Страница 153: ...ring the Module Flow Connections to the Vialsampler 7 Install the capillary from port 6 of the injection valve to the ICC or MCT The correct plumbing is shown in the figure below and is attached to th...

Страница 154: ...ng on the controller e g Agilent Local Controller OpenLab EZChrom Masshunter the screens look different Figure 29 ChemStation Method and Run Control After successful load of the OpenLab ChemStation yo...

Страница 155: ...ive areas If you move the mouse cursor across the icons tray EMF button the cursor will change and you may click on the icon to 1 Turn on off the sampler 2 Check which sample drawers are installed 3 G...

Страница 156: ...Method Parameter Settings on page 160 Injector Program When you activate a pretreatment injector program it replaces the standard injection cycle See Using an Injector Program on page 169 Identify Dev...

Страница 157: ...n Ready Error state and Not Ready text or Error text Offline gray Ok No Maintenance required green EMF warning Maintenance might be required yellow EMF warning Maintenance required red Table 15 The Vi...

Страница 158: ...ies Vialsamplers User Manual 158 4 Preparing the Module Setting up the Vialsampler Control Settings The control settings are available via right click on the active area of the grafical user interface...

Страница 159: ...ostat is installed and configured and the Constant temperature mode is selected Select On to switch on the cooler thermostat Specify the required temperature in the adjacent field The specified temper...

Страница 160: ...Settings The method parameter settings are available via right click on the active area of the grafical user interface Figure 31 Method parameter settings NOTE For additional help and support highligh...

Страница 161: ...enabled only if you have selected Flush Port Specify a flush time in seconds between 1 and 100 in steps of 1 Location enabled only if you have selected a Wash Vial Allows you to specify a wash locati...

Страница 162: ...for an injection cycle For viscous samples in combination with multi draw use a slow Eject Speed Wait Time After Draw ensures that the temporary vac uum which originates from the drawing of liquid fro...

Страница 163: ...to obtain the desired degree of flushing to prevent sample car ryover Overlapped Injection Mode provides faster throughput of samples by allowing the preparation of the next injection while the curre...

Страница 164: ...ector program comprises a series of numbered lines each specifying an operation that the autosampler carries out sequentially When you activate a pretreatment injector program it replaces the standard...

Страница 165: ...gs are available via menu Instrument Show ConfigUI Figure 32 Configuration view NOTE For additional help and support Highlight the desired area and press the F1 key A help screen will open with additi...

Страница 166: ...t 167 Using an Injector Program 169 General Recommendation for Lowest Carryover 170 Fast Injection Cycle and Low Delay Volume 171 Overlapped Injection Mode 171 General Recommendations for Fast Injecti...

Страница 167: ...utomated Needle Wash Wash Port The automated needle wash can be programmed either as method parameter enable needle wash or the needle wash can be included into the injector program When the automated...

Страница 168: ...re soluble and the vial should not be capped If the wash vial is capped small amounts of sample remain on the surface of the septum which may be carried on the needle to the next sample Injector Progr...

Страница 169: ...to ensure that the bypass grove is filled with the start concentration of the mobile phase Otherwise the separation could be influenced especially if microbore columns are used Example Outside wash o...

Страница 170: ...edure can be used to clean the needle seat In Lab Advisor go to Maintenance Change Needle this sets the needle into home position Remove the safety cover of the sampler Be carefully not to risk an inj...

Страница 171: ...o the column without passing sample loop needle and needle seat capillary This can help to have faster cycle times especially if low flow rates have to be used like it is mandatory in narrow bore and...

Страница 172: ...scribed in this section the first step to provide short cycle times are optimizing the chromatographic conditions If this is done the autosampler parameter should be set to Overlapped injection mode I...

Страница 173: ...e mobile phase is not degassed adequately small gas bubbles can form in the sample loop during the injection sequence When using injection volumes 2 L these gas bubbles can affect the injection volume...

Страница 174: ...ed to lower speed for optimum results A DRAW statement in an injector program also uses the draw speed setting which is configured for the autosampler Eject Speed The default eject speed setting is 40...

Страница 175: ...table for most applications However for applications using mobile phases with pH below 2 3 or above 9 5 the Vespel seal can degrade faster leading to reduced seal lifetime PEEK Seal for 1260 Infinity...

Страница 176: ...nd Troubleshooting Tools 179 Maintenance Positions 179 Gripper Verification 183 Injector Steps 184 Park Arm 186 Diagnostic Tests 187 Sample Cooler Function Test 187 Heater Test 188 Agilent Lab Advisor...

Страница 177: ...al impression of the actual state of the instrument The power indicator light is integrated into the main switch of the module and provides information about whether or not the system is powered on Wh...

Страница 178: ...ab Advisor Soft ware 1200 Infinity Instant Pilot InfinityLab Com panion Maintenance Posi tions Tool yes yes yes Park Arm Tool yes yes yes Injector Steps Tool yes yes yes Gripper Verifica tion Tool yes...

Страница 179: ...nger or the gripper arm to be moved into specific positions With the Maintenance Positions function these components can be moved into predefined service positions enabling the easy and safe access to...

Страница 180: ...position by slightly lifting it up and then flapping it to the left 6 Confirm that the wash port safety flap is in the service position and click Next to continue 7 Wait until the needle moves down a...

Страница 181: ...Function The Change Metering Seal and Piston function releases the tension on the metering spring by moving the plunger of the analytical head into the maintenance position The execution of this func...

Страница 182: ...e execution of this function encompasses the following elementary steps when the Agilent Lab Advisor Software is used 1 Select Maintenance Positions from the list under Service Diagnostics 2 Under Tas...

Страница 183: ...Click Pick Put Vial The gripper arm fetches the vial lifts it up and places it back to its original position 4 Repeat the preceding steps with a different vial position if needed 5 Select Back in the...

Страница 184: ...tool encompasses the following elementary steps when the Agilent Lab Advisor Software is used 1 Select Injector Steps from the list under Service Diagnostics 2 Enter a valid value for the vial locati...

Страница 185: ...e vial NOTE Always the default needle offset value 0 0 mm is applied Valve Bypass Needle Up Vial to Seat Draw The metering device draws the specified volume into the loop capillary NOTE This step can...

Страница 186: ...Park Arm function can be found under Special Commands in the Instrument Control tab of the Agilent Lab Advisor software CAUTION Unsecured transportation of the module The Vialsampler must be transpor...

Страница 187: ...he evaporator temperature sensor If the reading is stable for at least 10 s T 0 5 C the compressor turns on and the temperature inside the cooler thermostat starts to drop For the test to succeed the...

Страница 188: ...column oven and compares it against the threshold value The test takes less than 1 min to complete and returns a pass fail type result For the test to start the actual temperature in the column oven m...

Страница 189: ...c is included with every Agilent 1200 Infinity Series and Agilent InfinityLab LC Series instrument The Lab Advisor Advanced features can be unlocked by purchasing a license key and include real time m...

Страница 190: ...es 201 Command failed errors 201 Metering home failed 202 Automatic Referencing procedure failed 202 Needle up down failed errors 203 Missing vial errors 204 Motor overtemp 205 Motor alignment failed...

Страница 191: ...5 Cooler PCB is in error mode 215 Cooler condenser fan failed 216 Thermostat communication error 216 Heater defect 216 Heater in operating error 217 Thermostat sensor defect 217 Compressor has error 2...

Страница 192: ...notification all LEDs get red and the run will be stopped Depending on the module type this stop is implemented differently For example for a pump the flow will be stopped for safety reasons For a de...

Страница 193: ...s completed successfully and the timeout function switched off the module as requested Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required 2 A...

Страница 194: ...nother module with a CAN connection to the system Fix the leak in the external instrument before restarting the module 2 Leak detected in an external instrument with a remote connection to the system...

Страница 195: ...configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 Not ready condition in one of th...

Страница 196: ...changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capilla...

Страница 197: ...ent to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the power switch board P...

Страница 198: ...ak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective leak sensor Please contact...

Страница 199: ...er switch board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the power switch board is dependent on ambient temperature The change in resistan...

Страница 200: ...e error message is generated This limit is given by 2 revolutions second for longer than 5 seconds Depending on the module assemblies e g the lamp in the detector are turned off to assure that the mod...

Страница 201: ...specific information on the nature of the issue To identify these source error events look for entries with the same timestamp as for the Command failed error message Error ID 34202 Metering command f...

Страница 202: ...Blockage in the flow path Check if there is a significant pressure difference 10 15 bar between the mainpass and the bypass position of the injection valve If yes sys tematically replace the component...

Страница 203: ...on of the needle arm is not found or the needle arm is unable to move Probable cause Suggested actions 1 Physical blockage Look for potential sources of physical block age in the path of the needle ar...

Страница 204: ...verify that there is no controversial information specified there Rerun the method sequence and observe if the problem persists 2 Unsuitable vial type Verify that the vials and caps used by the metho...

Страница 205: ...s not hin dered by any obstacle for example by a vial that is stuck below the transport rods in the transport assembly body Restart the sampler and observe if the prob lem still persists 2 Broken grip...

Страница 206: ...rt assembly stepper motors failed during the initialization process 34221 0 corresponds to the X axis stepper motor 34221 1 corresponds to the Z axis stepper motor 34221 2 corresponds to the theta ste...

Страница 207: ...e respective error ID reported Error ID 34309 Invalid vial position Error ID 34313 Invalid wash vial position Probable cause Suggested actions 1 Missing vial tray Verify that the correct vial drawer t...

Страница 208: ...specific information on the nature of the issue To identify these source error events look for entries with the same timestamp as for the Cleanup after abort failed error message Wash procedure failed...

Страница 209: ...re failed Error ID 34316 Start of piston change procedure failed Error ID 34317 Start of gripper change procedure failed Injection valve position not found Error ID 34319 The injection valve position...

Страница 210: ...of the issue To identify these source error events look for entries with the same timestamp as for the Initialization failed error message Error ID 34322 Metering device initialization failed Error I...

Страница 211: ...age in the path of the gripper arm and eliminate them Restart the sampler and observe if the problem persists 2 Broken gripper Verify the correct functioning of the gripper by using the Gripper Verifi...

Страница 212: ...o the gripper finger movements Probable cause Suggested actions 1 Contaminated transport rods Inspect the x and z axis transport rods and clean them if you observe contamination or dust accu mulation...

Страница 213: ...The cooler thermostat was switched off due to a condensate event Probable cause Suggested actions 1 Potential hardware error Please contact your Agilent service representa tive Probable cause Suggest...

Страница 214: ...able cause Suggested actions 1 Overheated condenser Turn off the cooler thermostat and wait for 15 min to allow the system to cool down Verify if there is enough space around the sampler for adequate...

Страница 215: ...te sensor of the cooler thermostat is not working properly Cooler PCB is in error mode Error ID 30275 The system is in the error state because the compressor control board has encountered an unexpecte...

Страница 216: ...state because the communication between the sampler and the thermostat has failed Heater defect Error ID 30739 The heating function of the thermostat is not working properly Probable cause Suggested a...

Страница 217: ...nsors of the cooler thermostat is not working properly Compressor has error Error ID 30756 The system is in the error state because the control board of the compressor has encountered an unexpected er...

Страница 218: ...n Error ID 30768 Thermostat fan defect Error ID 30771 One of the cooling fans of the cooler thermostat is not working properly Probable cause Suggested actions 1 Potential hardware error Please contac...

Страница 219: ...the Integrated Column Compartment is switched due to overcurrent To verify the correct functioning of the heating element perform the Heater Test in the Agilent Lab Advisor software Probable cause Su...

Страница 220: ...is switched off because the trend analysis of the reading from the digital temperature sensor has failed To verify the correct functioning of the heating element perform the Heater Test in the Agilent...

Страница 221: ...n heater not connected Error ID 30825 The Integrated Column Compartment is either not connected or cannot be recognized by the system Probable cause Suggested actions 1 Connection problems Power off t...

Страница 222: ...Assembly 229 Exchange the Needle Seat Assembly 235 Exchange the Sample Loop Assembly 241 Exchange the Rotor Seal 245 Exchange the Metering Seal and Piston 250 Replace the Analytical Heads 255 Exchange...

Страница 223: ...n to Maintenance Introduction to Maintenance The module is designed for easy maintenance Maintenance can be done from the front with module in place in the system stack Figure 33 Overview of the Vials...

Страница 224: ...duct user guides or use of the products in violation of applicable laws rules or regulations Use your Agilent products only in the manner described in the Agilent product user guides WARNING Electrica...

Страница 225: ...laboratory practice The volume of substances should be reduced to the minimum required for the analysis Do not operate the instrument in an explosive atmosphere CAUTION Safety standards for external...

Страница 226: ...es on a contractual basis For information regarding the maintenance inspection contract contact your Agilent representative The following pages describe the maintenance simple repairs of the module th...

Страница 227: ...sing organic solvents for cleaning purposes They can cause damage to plastic parts WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module D...

Страница 228: ...scription Flat screwdriver Parts required p n Description G7129 68702 Door Kit for Infinity II Vialsampler Preparative Autosampler Preparations Finish any pending acquisition job 1 Press the release b...

Страница 229: ...1 Needle assembly 1290 Vialsampler for G7129 87012 needle seat OR 1 G7129 87202 Needle assembly slotted for high injection volumes Preparations Finish any pending acquisition job Stop the flow at the...

Страница 230: ...always exchange the needle assembly and the needle seat at the same time to prevent premature leakage NOTE When the instrument setup has changed configure the new setup in the online view of the Chro...

Страница 231: ...safety cover WARNING Sharp needle Uncovered needles may cause injuries Do not change the needle seat at this point Do not touch the tip of the needle 5 Move the wash port into the service position by...

Страница 232: ...2 8 Maintenance Exchange the Needle Assembly 7 Disconnect the sample loop from the needle and if needed also from the metering device 8 Loosen the fixing screw 9 Lift out the needle 10 Mount and align...

Страница 233: ...ake sure of the correct positioning of the loop capillary the uncoated part of the capillary must be horizontal NOTE Do not overtighten the fitting NOTE Incorrect positioning and installation of the n...

Страница 234: ...and fix the screw 2 16 In Lab Advisor select Next to move the needle into the needle seat and then Back to finish the maintenance procedure OR In the Local Controller select Continue to move the nee d...

Страница 235: ...sembly 1260 Vialsampler Vespel 0 17 mm OR 1 G7129 87112 Seat Assembly 1290 Vialsampler Vespel 0 12 mm Preparations Finish any pending acquisition job Stop the flow at the pump and remove the solvent l...

Страница 236: ...r software select Service Diagnostics Maintenance Positions Change Needle Loop and Seat click Start and wait until the needle assembly is in maintenance position OR In the Local Controller start the m...

Страница 237: ...e tip of the needle 5 Move the wash port into the service position by slightly lifting it up and then flapping to the left 6 In Lab Advisor use Next to move the needle in the down position ca 2 mm abo...

Страница 238: ...capillary fitting from the injection valve port 5 and ease out the needle seat from the leak tray with the help of a flathead screwdriver 11 Insert the new needle seat and press it firmly into posi t...

Страница 239: ...horizontal NOTE Do not overtighten the fitting NOTE Incorrect positioning and installation of the needle loop connection can result in damaging or breaking the sample loop 14 In Lab Advisor use Next t...

Страница 240: ...17 In Lab Advisor select Next to move the needle into the needle seat and then Back to finish the maintenance procedure OR In the Local Controller select Continue to move the nee dle into the needle s...

Страница 241: ...p 20 L Preparations Finish any pending acquisition job Stop the flow at the pump and remove the solvent lines from the eluent bottles to avoid spilling solvent Close the shutoff valves at the pump if...

Страница 242: ...ons Change Needle Loop and Seat click Start and wait until the needle assembly is in maintenance position OR In the Local Controller start the maintenance mode and select Change Needle Loop and Seat f...

Страница 243: ...s ca 2 4 mm above the seat 6 Disconnect the loop capillary from the metering device and from the needle 7 Install the new sample loop Make sure of the correct positioning of the loop capillary the unc...

Страница 244: ...10 Move the wash port back into its normal position by turning it to the right 11 Install the safety cover 1 and fix the screw 2 12 In Lab Advisor select Next to move the needle into the needle seat...

Страница 245: ...Vespel 1300 bar for 1290 Infinity II Injection Valve OR 1 0101 1416 Rotor Seal PEEK 600 800 bar for 1260 Infinity Injection Valve Preparations Finish any pending acquisition job Stop the flow at the...

Страница 246: ...8 Maintenance Exchange the Rotor Seal 1 Open the doors of the module 2 Remove all capillaries from the injection valve with a 1 4 inch wrench 3 Loosen each fixing bolt two turns at a time Remove the b...

Страница 247: ...th the appropri ate solvent If more stringent cleaning is required use a sonicator Inspect the remaining valve components for contamination Clean them as necessary If the stator head is scratched repl...

Страница 248: ...12 o clock position Ensure the ring sits flat on the valve body 8 Install the stator head Tighten the bolts alternately two turns at a time until the stator head is secure NOTE Do not over tighten the...

Страница 249: ...the Rotor Seal 9 Reconnect the capillaries and tubes to the valve ports The correct plumbing is shown below and on the safety cover of the module 10 Slide the waste tube into the waste holder in the...

Страница 250: ...Insert tool Cleaning tissue and appropriate solvent like isopropanol or methanol Parts required p n Description 0905 1717 Metering Seal 40 L OR 0905 1503 Metering Seal 100 L OR 0905 1294 Metering Seal...

Страница 251: ...iston click Start and wait until the metering plunger is in maintenance position OR In the Local Controller start the maintenance mode and select Change Metering Device function 2 Open the doors of th...

Страница 252: ...ernal construction slightly differs for the 900 L analyitical head refer to Figure 46 on page 296 8 Use a seal insert tool to carefully remove the seal 9 Clean the chamber with lint free cloth and an...

Страница 253: ...ide without the drain hole must be matching with the capil lary port sitting on the non truncated side of the analyti cal head 14 Install the metering head assembly in the autosampler Ensure that the...

Страница 254: ...Agilent InfinityLab LC Series Vialsamplers User Manual 254 8 Maintenance Exchange the Metering Seal and Piston 17 Close the doors 18 Perform a System Pressure Test...

Страница 255: ...pump if available NOTE If the sampler with 900 L metering device is linked to a 1260 pump such as the G7112B Binary Pump the 400 bar pressure limit must be set manually in the method parameter setting...

Страница 256: ...Ensure that the drain hole of the metering body is facing downwards Tighten the bolts alternately two turns at a time until the metering device is secure 7 Reinstall the capillaries 8 In Lab Advisor...

Страница 257: ...anual 257 8 Maintenance Replace the Analytical Heads 10 Close the doors Next Steps 11 Perform a System Pressure Test 12 If needed install a sample loop matching the volume of the new analytical head s...

Страница 258: ...inish any pending acquisition job NOTE Alternatively any other tool or item can be used that can be fitted into the block position slot of the transport arm for example a straightened paper clip or a...

Страница 259: ...otate the gripper arm slightly to the left or right until you find the block position 5 Press in the gripper arm release button and carefully rotate the gripper arm approximately 40 50 to the right wh...

Страница 260: ...ft or right until you find the block position 2 NOTE The gripper arm orientation is slightly different compared to before taking it off 9 Rotate the gripper arm approximately 40 50 to the left while f...

Страница 261: ...o avoid spilling solvent WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks Please observe appropriat...

Страница 262: ...eading to the wash port and the tubing coming from the solvent bottle 5 Push the new cartridge onto the motor shaft until the clips click into place 6 Connect the wash port tubing to the left side of...

Страница 263: ...60033 Safety Wash Port for Needle Station Preparations Finish any pending acquisition job Stop the flow at the pump and remove the solvent lines from the eluent bottles to avoid spilling solvent Close...

Страница 264: ...the safety cover screw WARNING Sharp needle Uncovered needles may cause injuries Do not touch the tip of the needle 5 Remove the safety cover WARNING Sharp needle Uncovered needles may cause injuries...

Страница 265: ...l the needle tip is ca 2 4 mm above the seat 8 Disconnect the sample loop from the needle and if needed also from the metering device 9 Loosen the fixing screw 10 Lift out the needle 11 Release the sp...

Страница 266: ...ssembly 15 Install the new wash port by mounting it in the respec tive brackets of the needle station housing The pin must be latched on the housing Check the movement of the flapping mechanism Check...

Страница 267: ...Make sure of the correct positioning of the loop capillary the uncoated part of the capillary must be horizontal NOTE Do not overtighten the fitting NOTE Incorrect positioning and installation of the...

Страница 268: ...eedle into the needle seat and then Back to finish the maintenance procedure OR In the Local Controller select Continue to move the nee dle into the needle seat and then Exit to finish the main tenanc...

Страница 269: ...nformation There is no specific information for this module When The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on th...

Страница 270: ...67 60005 Sample Cooler OR 1 G7167 60101 Sample Thermostat WARNING Flammable refrigerant Formation of flammable gas air mixtures inside the Sample Thermostat and laboratory Keep open fire or sources of...

Страница 271: ...k strain or injury by following all precautions for lifting heavy objects Ensure that the load is as close to your body as possible Ensure that you can cope with the weight of your load CAUTION Routin...

Страница 272: ...ntly tapping on the sides of the sampler can help to remove the last traces of condensate from the system 4 Remove the condensate tubing NOTE If there is still some condensate inside the cooler thermo...

Страница 273: ...the cables Do not bend or pinch the cables Make sure that the Sample Cooler Sample Thermostat fits perfectly in the sampler 8 Slide the cooler thermostat all the way into the sampler making sure that...

Страница 274: ...dule CAUTION Damage to the Sample Cooler Sample Thermostat Wait at least 30 min before switching on the compres sor of the cooler thermostat This allows the refrigerant and system lubrication to reach...

Страница 275: ...Trays 283 Cartesian Vial Drawers 283 Classic Vial Drawers 284 External Tray 285 Multidraw Kits 286 Multidraw Kit 286 Large Volume Injection Kit 286 Sample Thermostat Upgrade 287 Integrated Column Comp...

Страница 276: ...embly peristaltic pump cartridge rotor seal and finger caps p n Description G7129 87200 Needle assembly 1260 Vialsampler for G7129 87017 needle seat G7129 87201 Needle assembly 1290 Vialsampler for G7...

Страница 277: ...s Metering Seals for Analytical Heads p n Description G7129 60500 Sample Loop 100 L white coded G7129 60400 Sample Loop 40 L green coded G7129 60300 Sample Loop 20 L red coded p n Description 0101 141...

Страница 278: ...mmX 400 mm SL SL 1290 Vialsampler to MCT 5500 1238 Capillary ST 0 12 mm x 105 mm SL SL ICC 3 L to column 5500 1250 Capillary ST 0 17 mm x 120 mm SL SL long socket 1260 Vialsampler to ICC 6 L 5500 124...

Страница 279: ...Capillary ST 0 12 mmX 400 mm SL SL 2 3 5063 6506 Finger Caps 15 pk if ordered separately 3 2 5043 1013 Tubing Clip 4 1 5500 1411 Tubing Connector 180 ID 6 4 mm 5 pk if ordered separately 5 2 5500 122...

Страница 280: ...Agilent InfinityLab LC Series Vialsamplers User Manual 280 9 Parts and Materials for Maintenance Accessory Kits Figure 34 Accessory Kit for Vialsamplers 1 2 3 4 5 6 7...

Страница 281: ...ry ST 0 17 mm x 400 mm SL SL 2 3 5063 6506 Finger Caps 15 pk if ordered separately 3 2 5043 1013 Tubing Clip 4 1 5500 1411 Tubing Connector 180 ID 6 4 mm 5 pk if ordered separately 5 2 5500 1223 Tubin...

Страница 282: ...Agilent InfinityLab LC Series Vialsamplers User Manual 282 9 Parts and Materials for Maintenance Accessory Kits Figure 35 Accessory Kit for Vialsamplers 1 2 3 4 5 6 7...

Страница 283: ...nce Vial Drawers and Trays Vial Drawers and Trays Cartesian Vial Drawers Figure 36 Cartesian Vial Drawers Item p n Description 1 G7129 60010 Drawer for 66 x 2 mL Vials 2 G7129 60110 Drawer for 18 x 6...

Страница 284: ...contains Figure 37 Classic Vial Drawer Kit p n Description G7129 60210 Classic Drawer for 50 x 2 mL Vials Left G7129 60220 Classic Drawer for 50 x 2 mL Vials Right 1 2 NOTE Do not use classic vial dr...

Страница 285: ...es Vialsamplers User Manual 285 9 Parts and Materials for Maintenance Vial Drawers and Trays External Tray Figure 38 External tray p n Description G7129 60000 External Tray for 5 x 2 mL Vials G1313 27...

Страница 286: ...13 87308 Seat Capillary ST 1500 L ID 0 94 mm 0100 0900 Zero Dead Volume Union ST NOTE With the Multidraw Kit the maximum injection volume can be extended to 500 or 1500 L for the 100 L analytical head...

Страница 287: ...grade Sample Thermostat Upgrade InfinityLab Sample Thermostat Upgrade Kit G4761A contains Figure 39 Sample Thermostat p n Description G7167 60101 Sample Thermostat 5067 6208 Condensate Drainage Kit no...

Страница 288: ...0 60030 Integrated Column Compartment 3 L Heater G7130 60060 Integrated Column Compartment 6 L Heater G7116 68003 Column Holder Lamella 2 pk not shown NOTE The Integrated Column Compartment is always...

Страница 289: ...751A contains Figure 41 Column Identifier Kit p n Description 5067 5964 Column ID Tag Reader 5067 5917 InfinityLab Column Identification Tag NOTE The InfinityLab Column Identification Tags used in G71...

Страница 290: ...njection Upgrade Item p n Description 1 G7129 60083 Analytical Head Assembly 900 L G7129 87202 Needle assembly slotted for high injection volumes 3 G1313 87303 Capillary ST 0 17 mm x 400 mm S S Loop e...

Страница 291: ...s Item p n Description 1 Top cover 2 5043 1804 Name Plate Infinity II 1290 2 5043 1805 Name Plate Infinity II 1260 3 Side Cover left 4 Side Cover right 5 5043 0286 Base Cover Infinity II NOTE The top...

Страница 292: ...Agilent InfinityLab LC Series Vialsamplers User Manual 292 9 Parts and Materials for Maintenance Cabinet and Door Kits Figure 42 Cabinet Kit for Infinity II Vialsampler 3 6 5 1 2 4 7 8 9...

Страница 293: ...d Door Kits Door Kit Door Kit for Infinity II Vialsampler Preparative Autosampler G7129 68702 contains Figure 43 Door Kit for Infinity II Vialsampler Item p n Description 1 Door panel left 2 Door pane...

Страница 294: ...al 294 9 Parts and Materials for Maintenance Cabinet and Door Kits Door Insulation Kit Door Insulation Kit G7129 67003 contains Figure 44 Door Insulation Kit Item p n Description 1 Foam for Left Panel...

Страница 295: ...n Description G7129 60082 Analytical Head Assembly 100 L 1 G7129 27710 Analytical Head 100 L 2 0905 1503 Metering Seal 100 L 3 G7129 60182 Seal Support 100 L 4 0515 1052 Screw ST M3x0 5 Hex 2 5 mm 5...

Страница 296: ...ssembly 900 L Item p n Description G7129 60083 Analytical Head Assembly 900 L 1 G7129 27790 Analytical Head 900 L 2 0905 1294 Metering Seal 900 L 3 5001 3764 Seal Support 900 L 4 G7129 60006 Analytica...

Страница 297: ...gure 47 Analytical head assembly 40 L Item p n Description G7129 60084 Analytical Head Assembly 40 L 1 G7129 27704 Analytical Head 40 L 2 0905 1717 Metering Seal 40 L 3 G7129 60184 Seal Support 40 L 4...

Страница 298: ...ort Injection Valve 600 bar Figure 48 2 Position 6 Port Injection Valve 600 bar 800 bar Item p n Description 5067 4245 2 Position 6 Port Injection Valve 600 bar 1 5068 0018 Screws ST 8 32 Hex 9 64 10...

Страница 299: ...ort Injection Valve 800 bar Figure 49 2 Position 6 Port Injection Valve 600 bar 800 bar Item p n Description 5067 6689 2 Position 6 Port Injection Valve 800 bar 1 5068 0018 Screws ST 8 32 Hex 9 64 10...

Страница 300: ...ion 6 Port Injection Valve 1300 bar Figure 50 2 Position 6 Port Injection Valve 1300 bar Item p n Description 5067 4238 2 Position 6 Port Injection Valve 1300 bar 1 5068 0018 Screws ST 8 32 Hex 9 64 1...

Страница 301: ...lers User Manual 301 10 Identifying Cables Cable Overview 302 Analog Cables 304 Remote Cables 306 CAN LAN Cables 310 RS 232 Cables 311 USB 312 This chapter provides information on cables used with the...

Страница 302: ...iption 35900 60750 Agilent 35900A A D converter 01046 60105 Analog cable BNC to general purpose spade lugs p n Description 5188 8029 ERI to general purpose 5188 8044 Remote Cable ERI ERI 5188 8045 Rem...

Страница 303: ...elded 7 m for point to point connec tion p n Description RS232 61601 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers...

Страница 304: ...ables One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 35900 A D converte...

Страница 305: ...ables Analog Cables Agilent Module to BNC Connector Agilent Module to General Purpose p n 8120 1840 Pin BNC Pin Agilent module Signal Name Shield Shield Analog Center Center Analog p n 01046 60105 Pin...

Страница 306: ...on Cable 1 2 m D Sub15 pin male female p n 5188 8029 pin Color code Enhanced Remote Classic Remote Active TTL 1 white IO1 START REQUEST Low 2 brown IO2 STOP Low 3 green IO3 READY High 4 yellow IO4 POW...

Страница 307: ...8 8045 ERI to APG Connector D_Subminiature 15 pin ERI Connector D_Subminiature 9 pin APG p n 5188 8045 Pin ERI Signal Pin APG Active TTL 10 GND 1 1 Start Request 9 Low 2 Stop 8 Low 3 Ready 7 High 5 Po...

Страница 308: ...des a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Table 22 5188 8057 ERI to APG an...

Страница 309: ...rown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 B...

Страница 310: ...connected to Agilent modules CAN or LAN connectors CAN Cables LAN Cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m p n Descrip...

Страница 311: ...tion RS232 61601 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It is also called Null Modem Cable wit...

Страница 312: ...User Manual 312 10 Identifying Cables USB USB To connect a USB Flash Drive use a USB OTG cable with Mini B plug and A socket p n Description 5188 8050 USB A M USB Mini B 3 m PC Module 5188 8049 USB A...

Страница 313: ...the Module 318 Serial Number Information 318 Interfaces 319 Overview Interfaces 321 ERI Enhanced Remote Interface 323 USB Universal Serial Bus 325 Setting the 6 bit Configuration Switch 326 Special S...

Страница 314: ...erties are the complete communication capabilities CAN LAN USB and RS 232 memory management ability to update the firmware of the main system Main System Its properties are the complete communication...

Страница 315: ...er for example 1315B for the G1315B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 650 is revision 6 50 XXX is the build number of...

Страница 316: ...initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in spe...

Страница 317: ...odule from a computer through a LAN connection This connector is activated and can be configured with the configuration switch With the appropriate software the USB connector may be used to control th...

Страница 318: ...serial number information on the instrument labels provide the following information Configuration switch Power socket CAN ERI LAN USB Mini Port CCXZZ00000 Format CC Country of manufacturing DE Germa...

Страница 319: ...232 Analog APG A ERI E Special Pumps G7104A C 2 No Yes Yes 1 A G7110B 2 Yes Yes No No E G7111A B G5654A 2 Yes Yes No No E G7112B 2 Yes Yes No No E G7120A 2 No Yes Yes 1 A G7161A B 2 Yes Yes No No E S...

Страница 320: ...No No No No No Requires a host module with on board LAN with minimum FW B 06 40 or C 06 40 or with addi tional G1369C LAN Card G1364E F G5664B 2 Yes Yes No No E THERMOSTAT for G1330B Others G7116A B 2...

Страница 321: ...rface replaces the RS 232 Serial interface in new FUSION generation modules For details on USB refer to USB Universal Serial Bus on page 325 Analog Signal Output The analog signal output can be distri...

Страница 322: ...L circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal Table 24 ERI si...

Страница 323: ...tronics use ERI This interface is already used in the Agilent Universal Interface Box 2 UIB2 ERI Description The ERI interface contains eight individual programmable input output pins In addition it p...

Страница 324: ...d 1 Wire Data Future Use This serial line can be used to read out an EPROM or write into an EPROM of a connected ERI device The firmware can detect the connected type of device automatically and updat...

Страница 325: ...ailable by firmware command defined turn on off For devices that need higher power Class 0 0 5 A maximum 12 W Class 1 1 0 A maximum 24 W Class 2 2 0 A maximum 48 W Class depends on hosting module s in...

Страница 326: ...ics Switch settings provide configuration parameters for LAN and instrument specific initialization procedures All modules with FUSION electronics Default is ALL switches DOWN best settings Default IP...

Страница 327: ...settings are taken from non volatile memory 0 n a 2 2 not assigned Always keep these switches on position 0 off n a LAN Init Mode n a Use Default IP Address3 3 Default IP Address is 192 168 254 11 0...

Страница 328: ...start can be used to bring the module into a defined mode with default parameter settings Boot Main System Revert to Default Data The instrument will boot to main mode and changes to the module s defa...

Страница 329: ...ion Switch Table 26 Boot Resident Forced Coldstart SW1 SW2 SW3 SW4 SW5 SW6 Init Mode 1 0 0 0 0 0 Boot Main System Keep Data 1 1 0 0 0 0 Boot Resident System Keep Data 1 0 0 0 0 1 Boot Main System Reve...

Страница 330: ...pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are virtual elimination of fixing screws bolts or ties reducing...

Страница 331: ...e user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable...

Страница 332: ...hes 335 Initialization Mode Selection 336 Dynamic Host Configuration Protocol DHCP 338 General Information DHCP 338 Setup DHCP 339 Manual Configuration 341 With Telnet 342 PC and Agilent ChemStation S...

Страница 333: ...an be found on a label at the rear of the module underneath the configuration switch see Figure 56 on page 333 Figure 55 MAC Label 2 Connect the instrument s LAN interface see Figure 56 on page 333 to...

Страница 334: ...ased DHCP Server using the so called Dynamic Host Configuration Protocol This mode requires a LAN onboard Module or a G1369C LAN Interface card see Setup DHCP on page 339 by manually setting the param...

Страница 335: ...ration Switches The configuration switch can be accessed at the rear of the module Figure 57 Location of Configuration switch example shows a G7114A B VWD The module is shipped with all switches set t...

Страница 336: ...ode Using Stored is selected the parameters are taken from the non volatile memory of the module The TCP IP connection will be established using these parameters The parameters were configured previou...

Страница 337: ...may then close the session select the initialization mode Using Stored power on again and establish the TCP IP connection using the new parameters When the module is wired to the PC directly e g using...

Страница 338: ...rs obtained become the active parameters immediately They are not stored to the non volatile memory of the card Besides requesting the network parameters the card also submits its hostname to the DHCP...

Страница 339: ...is on a label on the card or at the rear of the mainboard for example 0030d3177321 On the Local Controller the MAC address can be found under Details in the LAN section Figure 61 LAN Setting on Instan...

Страница 340: ...OpenLAB CDS ChemStation Edition Lab Advisor Firmware Update Tool and use MAC address as host name e g 0030d3177321 The LC system should become visible in the control software see Note in section Gene...

Страница 341: ...volatile memory of the module It never affects the currently active parameters Therefore manual configuration can be done at any time A power cycle is mandatory to make the stored parameters become th...

Страница 342: ...king on Windows START button and select Run Type cmd and press OK 2 Type the following at the system DOS prompt c telnet IP address or c telnet host name Figure 63 Telnet Starting a session where IP a...

Страница 343: ...en press Enter where parameter refers to the configuration parameter you are defining and value refers to the definitions you are assigning to that parameter Each parameter entry is followed by a carr...

Страница 344: ...bout the LAN interface MAC address initialization mode Initialization mode is Using Stored active TCP IP settings TCP IP status here ready connected to PC with controller software e g Agilent ChemStat...

Страница 345: ...ilent ChemStation Setup PC Setup for Local Configuration This procedure describes the change of the TCP IP settings on your PC to match the module s default parameters in a local configuration see Tab...

Страница 346: ...lent InfinityLab LC Series Vialsamplers User Manual 346 12 LAN Configuration PC and Agilent ChemStation Setup 2 You may enter here the fixed IP address of the module or use the Alternative Configurati...

Страница 347: ...C Series Vialsamplers User Manual 347 12 LAN Configuration PC and Agilent ChemStation Setup 3 We will use the direct LAN access via Cross over LAN cable with the module s IP address 4 Click on OK to s...

Страница 348: ...tup for the 1290 Infinity system using the 1290 Infinity DAD G4212A as the interfacing module The setup works in the same way for all other systems 1 Open the ChemStation Configuration Editor 2 Select...

Страница 349: ...ries Vialsamplers User Manual 349 12 LAN Configuration PC and Agilent ChemStation Setup 5 Click on OK 6 Select LC System Access Access Point and click on Add 7 Click on OK The Configuration Editor sho...

Страница 350: ...ations 9 Save the current configuration via File Save 10 Exit the Configuration Editor 11 Start the Agilent ChemStation During first startup or when the system configuration has changed a notification...

Страница 351: ...ion Setup 13 Enter the IP address or the Hostname of the module with the LAN access 14 Click on OK The selected module is shown now in the right window with serial number In addition all other modules...

Страница 352: ...u may see the details of the module by selecting the module and clicking on Configure Under Connection Settings you may change the IP Hostname of the module may require a re start of the ChemStation A...

Страница 353: ...Agilent InfinityLab LC Series Vialsamplers User Manual 353 12 LAN Configuration PC and Agilent ChemStation Setup Figure 69 Screen After Successful Load of ChemStation...

Страница 354: ...6 Do Not Operate in an Explosive Atmosphere 357 Do Not Remove the Instrument Cover 357 Do Not Modify the Instrument 357 In Case of Damage 357 Solvents 358 Symbols 359 Waste Electrical and Electronic E...

Страница 355: ...sumes no liability for the customer s failure to comply with these requirements Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufac...

Страница 356: ...compliance with safety or EMC regulations Make all connections to the unit before applying power NOTE Note the instrument s external markings described under Symbols on page 359 WARNING Missing electr...

Страница 357: ...Presence of flammable gases or fumes Explosion hazard Do not operate the instrument in the presence of flammable gases or fumes WARNING Instrument covers removed Electrical shock Do Not Remove the In...

Страница 358: ...tment For the solvents methanol and ethanol keep the solvent temperature at least 25 K below the boiling point Do not operate the instrument in an explosive atmosphere Do not use solvents of ignition...

Страница 359: ...potential GWP see instrument label Flammable Material For Sample Thermostat which uses flammable refrigerant consult Agilent Information Center User Manual before attempting to install or service thi...

Страница 360: ...om devices and objects that could be damaged by strong magnetic fields Indicates a pinching or crushing hazard Indicates a piercing or cutting hazard Table 32 Symbols WARNING A WARNING alerts you to s...

Страница 361: ...WEEE Directive This product complies with the European WEEE Directive marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic househol...

Страница 362: ...lent Infinity II Sample Cooler Table 33 Physical properties of refrigerant HFC 134a Molecular weight 102 Critical temperature 101 1 C Critical pressure 40 6 bar Boiling point 26 5 C Table 34 Physical...

Страница 363: ...nd consult a doctor HFC 134a must not be allowed to escape in enclosed areas Although HFC 134a is not toxic there is a danger of suffocation as gaseous refrigerant is heavier than air Please observe t...

Страница 364: ...company All media must be disposed of in accordance with national and local regulations Please contact your local Agilent Service Center in regard to safe environmental disposal of the appliance or c...

Страница 365: ...ilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and...

Страница 366: ...eclaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position 70 d...

Страница 367: ...oric acid or phosphate buffer which are less corrosive against stainless steel halogenated solvents or mixtures which form radicals and or acids for example 2CHCl3 O2 2COCl2 2HCl This reaction in whic...

Страница 368: ...Vialsamplers User Manual 368 13 Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Inte...

Страница 369: ...e Agilent 1260 Infinity II Vialsampler G7129A the Agilent 1290 Infinity II Vialsampler G7129B and the Agilent 1260 Infinity II Vialsampler G7129C The manual describes the following Introduction site r...

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