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126 1200 Series Handheld Control Module Users’s Guide
10 Running a Gradient Analysis
Entering Settings
Entering Settings
To set up the gradient analysis you will set the LC system settings to default
and then modify selected settings, the other settings will remain with their
default values. You will then save these settings to a method called GRAD.
To set up the solvent gradient, you will create a timetable in the method. This
is done using the Timetable screen, accessed by selecting the Timetable button
[F2] in the Pump settings screen.
The example assumes that the Timetable is empty. If the Timetable is not
empty, use the All Lines item, accessed by pressing the Delete button.
1
Enter the
Analysis
screen.
2
Set the Vial Range as 1 to 1.
3 Set the Number of Injections to 1.
4
Skip the method name field
5
Select the
Settings
button [ F1 ].
6 Select LC System from the menu.
7
Press
Enter
.
8
Select the
Default
button [ F7 ] and select the Yes button to load defaults.
9 Press the Esc key to enter the Analysis screen.
10 Enter the following values in the Analysis screen.
11 Select the Settings button [ F1 ].
12 Select BIN or QUAT pump from the menu.
Table 5 Values in the Analysis Screen
Setting Value
Flow 1.5 ml/minute
Detection wavelength 254 nm
Injection volume 1 µl
Stoptime 5 min