8
Run the System
Run the Experiment
2D-LC User Guide
210
Run the Checkout Familiarization Procedure for High
Resolution (LC-LC)
To run the checkout, various hardware configurations are possible, see
on page 56. Not all options can be shown. As example the
is used here.
The following parameters have been optimized for this standard configuration.
Parameters can deviate slightly for your system. Run the experiment and
subsequently edit or optimize the method for your setup.
Table 21
Recommended conditions in 1D (HPLC) for SHC, MHC, and HiRes 2D-LC
Parameter
Value
1
D Column Compartment (MCT)
Column
RRHD SB-C18, 2.1x 100 mm, 1.8 µm, 1200 bar (858700-902)
Column temperature
40 °C
Stop time
As pump/No limit
1
D Pump
Mobile Phase A
Water + 0.2 % formic acid
Mobile Phase B
Methanol
Flow Rate
0.6 mL/min
Post time
6 min
Mobile Phase
Gradient:
20 % B 0.00 min
100 % B 50 min
Autosampler
Injection Volume
2 µLfor Standard LC
0.5 µL Positive Mode for LCMS
10 µL Negative Mode for LCMS
Injection Needle Wash In Flush Port, 10 s, acetonitrile/water (50/50)
Stop time
As pump/No limit
1
D Detector (DAD)
Diode-array Detector
Signal A
254 nm, Bandwidth 4 nm
Reference Wavelength 360 nm
Reference Bandwidth
100 nm
Peak width
20 Hz
Stop time
Stop time As pump/No limit
Содержание 1290 Infinity II 2D-LC Solution
Страница 122: ...5 2D LC Data Acquisition in MassHunter Workstation 11 Instrument Details 2D LC User Guide 122 3 Click Screen 4 Click OK...
Страница 263: ...2D LC User Guide 263 9 Data Analysis GC Image Basic Information 3 Import parameters...
Страница 264: ...9 Data Analysis GC Image Basic Information 2D LC User Guide 264 4 Fit view...
Страница 267: ...2D LC User Guide 267 9 Data Analysis GC Image Basic Information 11 View single 2D chromatograms 12 Select blobs...
