Package
Insert
For Research Use Only
Not for use in diagnostic procedures.
Product Description
Axiom
®
Microbiome Array Plate is designed for the detection of microbial
organisms within samples. This array plate, when used with Axiom
®
2.0 Reagent
Kit (P/N 901758 and 902910) enables researchers to perform small or large-scale
microbiome profiling studies with minimal hands-on processing per plate.
Each plate consists of 24 or 96 microarrays and is offered as a part of a complete
automated solution from Affymetrix that includes instrumentation for sample
preparation, array washing, staining and scanning.
Microbial genomes are amplified using the Axiom 2.0 Reagent Kit and are
present as short randomly generated fragments of genomic DNA (25 to 125
base pair) prior to array hybridization. The reagents for processing Axiom arrays
are also included in this reagent kit.
Axiom Microbiome Array Plates are: For research use only. Not for use in
diagnostic procedures.
Oligonucleotide probes on Axiom array plates are synthesized
in situ
using
Affymetrix’ photolithographic process.
Instructions for Use
Refer to the following documents for instructions on processing samples using
the Axiom
®
2.0 Assay.
Related Documents
■
■
Axiom
®
Microbiome Solution User Guide
, P/N 703408
The following documents contain information related to the instruments and
software required to perform the Axiom assay:
■
■
Axiom
®
2.0 Assay on the Affymetrix
®
NIMBUS
®
Target Preparation
Instrument
, P/N 703349
■
■
Axiom
®
2.0 Assay for Affymetrix
®
NIMBUS
®
Site Preparation Guide
, P/N
703350
■
■
Axiom
®
2.0 Assay for Affymetrix
®
NIMBUS
®
Automated Target Prep Protocol
QRC
, P/N 703351
■
■
Axiom
®
2.0 Assay Manual Workflow User Guide
, P/N 702990
■
■
Axiom
®
2.0 Assay Manual Workflow Site Preparation Guide
, P/N 702991
■
■
Axiom
®
2.0 Assay Manual Target Prep Protocol QRC
, P/N 702989
■
■
Axiom
®
2.0 Assay for 24 Samples Manual Workflow User Guide
, P/N 703335
■
■
Axiom
®
2.0 Assay for 24 Samples Manual Workflow Site Preparation Guide
,
P/N 703336
■
■
Axiom
®
2.0 Assay for 24 Samples
Manual Target Preparation Protocol
QRC,
P/N 703337
■
■
Axiom
®
2.0 gDNA Sample Prep Protocol QRC
, P/N 702987
■
■
GeneTitan
®
MC Protocol for Axiom 2.0 Array Plate Processing QRC
,
P/N 702988
■
■
GeneTitan
®
Multi-Channel Instrument User Guide
, P/N 08-0306
■
■
GeneTitan
®
Multi-Channel Instrument Site Preparation Guide
, P/N 08-0305
■
■
Affymetrix
®
GeneChip
®
Command Console
®
Software User Guide
,
P/N 702569
Library Files
Library files contain information about the array design characteristics, probe use
and content, and scanning and analysis parameters. These files are unique for
each array type. Additional information can be located under the specific array
product on the Affymetrix website at:
www.affymetrix.com/support/technical/libraryfilesmain.affx
Reagents, Instrumentation, and Software Required
1.
Axiom
®
2.0 Reagent Kit
2.
Axiom
®
Microbiome Array Plate
3.
Affymetrix
®
NIMBUS
®
Target Preparation Instrument
4.
GeneTitan
®
Multi-Channel Instrument
5.
Axiom
™
MiDAS
®
For a complete list of reagents and consumables required, please refer to the
Axiom
®
Microbiome Solution User Guide
, P/N 703408.
Precautions
1.
AXIOM ARRAY PLATES ARE FOR RESEARCH USE ONLY. NOT FOR USE IN
DIAGNOSTIC PROCEDURES.
2.
Avoid microbial contamination, which may cause erroneous results.
3.
WARNING: All biological specimens and materials with which they
come into contact should be handled as if capable of transmitting
infection and disposed of with proper precautions in accordance with
federal, state, and local regulations. This includes adherence to the
OSHA Bloodborne Pathogens Standard (29 CFR 1910.1030) for blood-
derived and other samples governed by this act. Never pipet by
mouth. Avoid specimen contact with skin and mucous membranes.
4.
CAUTION:
Exercise standard precautions when obtaining, handling, and
disposing of potentially carcinogenic reagents.
5.
Exercise care to avoid cross-contamination of samples during all steps of this
procedure, as this may lead to erroneous results.
6.
Use powder-free gloves whenever possible to minimize introduction of
powder particles into sample or array plates.
7.
CAUTION:
Use care when handling the scan tray as it has protruding
guiding posts that may be sharp and can stick out of the pouch if not
handled carefully.
Ordering Information
P/N
Product Name
Description
Arrays
902903 Axiom
®
Microbiome Array Plate
One 24-array plate
902904 Axiom
®
Microbiome Array Plate
One 96-array plate
Supporting Products
902910
Axiom
®
Microbiome Reagent Kit for
four 24-array plates
Sufficient for four 24-Axiom
Microbiome Array Plates
901606 Axiom
®
GeneTitan
®
Consumables Kit
1
Contains all GeneTitan
consumables required to process
one Axiom array plate
901758
Axiom
®
2.0 Reagent Kit
Sufficient for one 96-Axiom
Microbiome Array Plate
1. One hyb tray, one scan tray, and five stain trays with covers are included for use with each array
plate. These consumables are required for processing Axiom array plates on the GeneTitan Multi-
Channel Instrument.
Patents
Products may be covered by one or more of the following patents: U.S. Patent
Nos. 7,332,273; 7,790,389; 8,114,584; 8,273,304; 8,501,122 and other U.S.
or foreign patents.
Trademarks
Affymetrix
®
, Axiom
®
, GeneChip
®
, Command Console
®
, CytoScan
®
, DMET
™
,
Eureka
™
, Eureka Genomics
®
, Eureka Genotyping
™
, Expression Console
™
,
GeneAtlas
®
, GeneChip-compatible
™
, GeneTitan
®
, Genotyping Console
™
,
myDesign
™
, MyGeneChip
™
, NetAffx
®
, OncoScan
®
, Powered by Affymetrix
™
,
PrimeView
®
, and ViewRNA
®
are trademarks or registered trademarks of
Affymetrix, Inc. Please see
www.affymetrix.com/trademarks
for a complete list of
Affymetrix trademarks. All other trademarks are the property of their respective
owners.
Axiom
®
Microbiome Array Plate
