How to clean an ACQUITY UPLC ELS Detector - WKB230245
OBJECTIVE or GOAL
Clean the ELS detector—as part of routine maintenance or in response to degraded performance.
ENVIRONMENT
• ACQUITY UPLC ELS Detector
PROCEDURE
Cleaning the drift tube
1. In the console, select ELS Detector from the system tree.
2. In the temperatures area, click the Nebulizer field, which contains an underlined set point value. The ELSD Nebulizer
Setup dialog box appears.
3. Select On from the nebulizer mode drop-down list.
4. Specify a nebulizer power percent level of 75%.
5. In the temperatures area, click the Drift Tube field, which contains an underlined set point value. The ELSD Drift Tube
Temperature dialog box appears.
6. Specify a drift tube heater temperature set point of 100 °C (212 °F), and then click OK
.
7. Remove the column.
8. Flush the system with 100% HPLC-grade water or a mixture of organic solvent compatible with your mobile phase for
60 minutes at 1 mL/min.
9. Reset the system to operating conditions.
10. Reinstall the column.
11. Evaluate chromatography.
Cleaning the nebulizer using sonication
1. Stop the liquid flow, and remove the solvent inlet line.
2. Power-off the detector and disconnect the power cable from the rear panel.
3. Open the detector door, gently pulling its right edge toward you.
4. Use a 5/16-inch wrench to loosen the compression screw that holds the inlet tubing in place, and then disconnect the
solvent inlet tubing.
Updated: Wed, 31 Aug 2022 14:32:45 GMT
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