Abbott CELL-DYN 3200, Руководство оператора системы

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CELL-DYN ® 3200 System Operator’s Manual
14-63
9140181H—October 2001
Section 14 Reticulocyte Package
Specimen Preparation
CAUTION: When using the reticulocyte reagent, avoid contact with skin
and clothing. This reagent contains New Methylene Blue, which will stain
skin, clothing, and many other surfaces.
WARNING: Potential Biohazard. Consider all clinical specimens and
controls that contain human blood or serum as potentially infectious. Use
established, good laboratory working practices when handling these
specimens. Wear appropriate personal protective equipment and follow
other biosafety practices as specified in the OSHA Bloodborne Pathogen
Rule or other equivalent biosafety procedures.
Procedure: Specimen Preparation
1. The Reticulocyte Package is available for use only in the Open Sampler
Mode.
2. Use reticulocyte reagent and verify the expiration date. Store the stock
reagent in the dark at room temperature.
3. Label a tube of reticulocyte reagent for each patient.
4. Verify that the whole blood specimen is warmed to room temperature and
well mixed prior to sampling.
5. Pipette 20 µL of the whole blood specimen into each labeled tube of
reticulocyte reagent.
6. Incubate the stained Reticulocyte specimens on the rotator or in a rack, after
fully inverting the stained specimens 5 times. Incubation must be performed
according to the reagent package insert.
NOTE: The stained Reticulocyte specimens must incubate for 15 minutes
but no more than 2 hours prior to processing on the
CELL-DYN 3200 System.
This timing will allow the Reticulocyte specimens to be processed for STAT
requests. Reticulocyte specimens can also be grouped and run in batches, provided
that the 2-hour incubation time limit is not exceeded.
Specimen Analysis
Specimen analysis is performed in the Open Mode.
NOTE: To ensure optimal flagging, the CBC must have been run within 8 hours
prior to the Retic run on the same analyzer using the same specimen.
NOTE: Specimen IDs must match exactly and are case sensitive.
Procedure: Running Patient Samples
1. Press the
[RETIC RUN]
key to display the
RETIC SPECIMEN TYPE
menu. Press
the
[PATIENT SPECIMEN]
key to display the
RETIC PATIENT SPECIMEN
screen.
2. From the
RETIC PATIENT SPECIMEN
screen enter the Specimen ID and press
the Enter key on the keyboard to start the search process. The Standard
Hematology Data Log for the last 8 hours will be searched for this Specimen
ID.