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14-60
CELL-DYN
®
3200 System Operator’s Manual
9140181H—October 2001
Reticulocyte Package
Routine Operation
Section 14
NOTE:
Refrigerated samples must be brought to room temperature before
mixing; this avoids damaging any fragile cells.
For the Absolute Reticulocyte calculation, it is recommended that the RBC value
used must be selected from the same specimen that will be used for the
Reticulocyte count and preferably run on the same analyzer.
The Reticulocyte Package will select RBC values from the Data Log for specimens
processed within the last 8 hours.
NOTE:
Specimen IDs must match exactly and are case sensitive.
If a specimen is more than 8 hours old and the CBC was processed more than 8
hours prior to performing the Reticulocyte analysis, manual entry of the RBC value
is an option.
If the RBC value is not entered, only the % Reticulocyte value will be obtained.
Interfering Substances
The CELL-DYN 3200 Reticulocyte method is a nucleic acid staining method.
Therefore, other substances that contain nucleic acids could potentially be
enumerated by the instrument as reticulocytes. If these interfering substances are
present in sufficient numbers, they may interfere with the dynamic thresholds used
to obtain the CELL-DYN 3200 reticulocyte count. Consequently, these specimens
should be flagged by the instrument. Refer to
Troubleshooting, Instrument Alert
Conditions
within this chapter for a complete description of the Reticulocyte flags.
The information in the following table, based on NCCLS Document H44-A
1
indicates substances that are known or potential interference. The
CELL-DYN 3200 Reticulocyte procedure is designed to minimize some common
interference, including high WBC counts and NRBCs.
Running
Background Counts
The reticulocyte background count should be included in the daily start-up
procedures to check for particulate matter in the reticulocyte reagent and the
CELL-DYN 3200 System. The background count is determined from the total
counts that occur in the reticulocyte scatter area on the 10°/90° scatterplot.
Table 14.1 Known or Potential Interferents
Cellular Elements
Cellular Inclusions
Miscellaneous
Platelet clumps
Basophilic stippling
Giant platelets
Leukocytes and
leukocyte fragments
Nucleated erythrocytes
Howell-Jolly bodies
Heinz bodies
Pappenheimer bodies
Parasites (malaria,
babesia)
Abnormal red cells
Paraproteins
Cold agglutinins
Platelet/erythrocyte
coincidence
Hemolysis
Содержание CELL-DYN 3200
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Страница 372: ...9 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Service and Maintenance Overview Section 9 NOTES ...
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