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CELL-DYN
®
3200 System Operator’s Manual
6-9
9140181H—October 2001
Section 6
Calibration Procedures
Sample Age
• All blood samples should be less than four hours old when calibration
begins.
1
These samples must be less than eight hours old by the time
calibration is completed.
• No more than two hours should elapse between the CELL-DYN 3200 run and
the assay by reference methodology or reference instrument. If samples are
run on the CELL-DYN 3200 first, assay by reference methodology should be
completed within one hour. (Certain reference methodologies are sensitive to
RBC swelling caused by in vitro deoxygenation.)
Sample Amount
The whole blood sample volume should be at least 12 mL to accomplish the
following:
• Obtain reference values on a reference instrument prior to calibration.
• Calibrate both the Open and Closed Modes.
• Confirm calibration of both modes.
NOTE:
Because only one fresh whole blood sample is used in Auto-Cal, it
is important that a representative sample be selected to calibrate the
instrument. A sample containing abnormalities may adversely
affect calibration.
Number of Cycles
• Samples should be assayed first on a reference instrument or by reference
methodology and then on the CELL-DYN 3200.
• Auto-Cal Method: A single fresh whole blood sample should be cycled for
10 consecutive runs when calibrating in either Open or Closed Mode.
• Enter Factor Method: Five fresh whole blood samples should be cycled twice
each for a total of at least ten runs when calibrating in either Open or Closed
Mode. Additional samples and/or repetitions of the specimens may be used
to achieve calibration accuracy beyond NCCLS recommendations.
Pre-Calibration Check List
Follow the procedures outlined in the Pre-Calibration Check List to ensure the
instrument is ready for calibration. Use the Problem List to note any problems
encountered. Make copies of both lists as needed.
NOTE:
Always complete the Pre-Calibration procedures before beginning any
calibration.
Содержание CELL-DYN 3200
Страница 12: ...xii CELL DYN 3200 System Operator s Manual 9140181K July 2002 NOTES ...
Страница 52: ...1 4 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Use or Function Overview Section 1 NOTES ...
Страница 132: ...3 38 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Principles of Operation References Section 3 NOTES ...
Страница 154: ...5 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Operating Instructions Overview Section 5 NOTES ...
Страница 234: ...5 82 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Operating Instructions Run Menu Section 5 NOTES ...
Страница 300: ...6 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Calibration Procedures Overview Section 6 NOTES ...
Страница 356: ...6 58 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Calibration Procedures References Section 6 NOTES ...
Страница 362: ...8 2 CELL DYN 3200 System Operator s Manual 9140181K July 2002 Hazards Overview Section 8 NOTES ...
Страница 370: ...8 10 CELL DYN 3200 System Operator s Manual 9140181K July 2002 Hazards References Section 8 NOTES ...
Страница 372: ...9 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Service and Maintenance Overview Section 9 NOTES ...
Страница 532: ...11 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Quality Control Overview Section 11 NOTES ...
Страница 558: ...11 28 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Quality Control References Section 11 NOTES ...
Страница 560: ...12 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Printers Overview Section 12 NOTES ...
Страница 566: ...13 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Sample Loader Overview Section 13 NOTES ...
Страница 590: ...14 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Reticulocyte Package Overview Section 14 NOTES ...
Страница 662: ...A 4 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Bar Codes Overview Appendix A NOTES ...
Страница 666: ...A 8 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Bar Codes Specifications Appendix A NOTES ...