Hardware Guide
4000 QTRAP
®
System
D1000092200 B
May 2010
Страница 1: ...Hardware Guide 4000 QTRAP System D1000092200 B May 2010 ...
Страница 2: ...r manufacturers and or their products which may contain parts whose names are registered as trademarks and or function as trademarks of their respective owners Any such usage is intended only to designate those manufacturers products as supplied by AB SCIEX for incorporation into its equipment and does not imply any right and or license to use or permit others to use such manufacturers and or thei...
Страница 3: ... Small Molecules 6 Qualitative Analysis of Proteins and Peptides 6 How the System Works 6 Features of the System 7 Parts of the Instrument 7 Component Descriptions 8 Shutting Down and Turning on the System 9 Instrument Safe Fluids 11 Source Gas Parameters 11 Turbo V Ion Source Settings 11 Compound Parameters 12 Related Documentation 13 Technical Support 13 Chapter 2 4000 Series Parameters 15 ...
Страница 4: ...Contents 4 Document Number D1000092200 B ...
Страница 5: ...olecules Qualitative analysis of small molecules Qualitative analysis of proteins and peptides Quantitative Analysis of Small Molecules This application involves measurement of specific molecular weight compounds usually a drug or metabolite in urine or other bodily fluid and their resulting fragment ions for determining the exact quantity of the compound in the sample of interest Quantitation is ...
Страница 6: ...ate identification of the sample using protein database searching For more detailed structural analysis using tandem mass spectrometry MS MS EPI Enhanced Product Ion scans are performed How the System Works The 4000 QTRAP system has a number of enhanced modes of operation A common feature of the enhanced modes is that ions are trapped in the Q3 quadrupole region and then scanned out to produce ful...
Страница 7: ...des of operation is rapid since it involves only the addition or removal of the resolving DC voltages The 4000 QTRAP system offers the following benefits over a standard triple quadrupole and a traditional 3 D ion trap mass spectrometer The proven quadrupole quantitation capabilities of the API 4000 instrument with additional scan types Enhanced MS Enhanced Multiply Charged Enhanced Product Ion En...
Страница 8: ...ssis Gas and Vacuum Panel The vacuum lines to the external roughing pump are connected through the gas and vacuum panel The panel also houses the gas supply connections and the external connection for the source exhaust pump Control Panel The control panel contains the main power switch four fuses and two Indicator Reset buttons Source Exhaust System Most ion sources produce both sample and solven...
Страница 9: ...turning off the instrument s main power switch When the main power switch is turned off the turbo pumps continue to rotate without power for a few minutes and continue to provide vacuum to the vacuum chamber If during this time the roughing pump is turned off the pressure in the vacuum line between the roughing pump and the turbo pumps increases The increase in back pressure can create an incorrec...
Страница 10: ...isfied before the turbo pumps will be initiated The Curtain Gas supply must be turned on at the source and the roughing pump must be turned on manually Interlocks pressure switches monitored by the firmware detect if the Curtain Gas supply and the roughing pump are switched on If the interlocks are not satisfied the turbo pumps are not initiated CAUTION If the ion source is removed the system elec...
Страница 11: ... Source Settings Table 1 3 shows the recommended starting values for the X and Y axis parameters For more information see the Turbo V Ion Source Operator s Guide Note This list is not complete If you are uncertain about a specific fluid do not use the fluid until confirmation is received from AB SCIEX that it will not present a hazard Note We recommend that you run the instrument with the Curtain ...
Страница 12: ...nd Fixed LIT Fill Time Use the preset value DFT Dynamic Fill Time Either select or deselect the feature based on your experiment In the Tools Settings Method Options dialog box the Dynamic Fill Time settings are optimized for the 10000 Da s scan speed These settings are also suitable for other LIT scan speeds Q0 Trapping Either select or deselect the feature based on your experiment The recommende...
Страница 13: ...rams AB SCIEX A complete list of the available documentation can be found in the online Help To view the Analyst software Help press F1 Technical Support AB SCIEX and its representatives maintain a staff of fully trained service and technical specialists located throughout the world They can answer questions about the instrument or any technical issues that may arise For more information visit the...
Страница 14: ...System Overview 14 Document Number D1000092200 B ...
Страница 15: ...o 5500 4500 to 0 4500 to 0 4500 to 0 NC 3 NC 3 3 3 3 3 3 3 0 to 5 0 to 5 0 to 5 5 to 0 5 to 0 5 to 0 TEM 2 3 TEM 2 3 0 0 0 0 0 0 0 to 750 0 to 750 0 to 750 0 to 750 0 to 750 0 to 750 OR DP 20 20 20 20 20 20 DP OR 0 to 400 0 to 400 0 to 400 400 to 0 400 to 0 400 to 0 Q0 EP 10 10 10 10 10 10 EP Q0 15 to 2 15 to 2 15 to 2 2 to 15 2 to 15 2 to 15 IQ1 IQ1 Q0 1 Q0 1 Q0 1 Q0 1 Q0 1 Q0 1 IQ1 Q0 offset 0 5...
Страница 16: ...0 0 400 to 0 400 to 0 400 to 0 0 to 400 0 to 400 0 to 400 CEM CEM 1800 1800 1800 1800 1800 1800 500 to 3297 500 to 3297 500 to 3297 500 to 3297 500 to 3297 500 to 3297 GS1 GS1 20 20 20 20 20 20 0 to 90 0 to 90 0 to 90 0 to 90 0 to 90 0 to 90 GS2 GS2 0 0 0 0 0 0 0 to 90 0 to 90 0 to 90 0 to 90 0 to 90 0 to 90 ihe 4 ihe 1 1 1 1 1 1 0 or 1 0 or 1 0 or 1 0 or 1 0 or 1 0 or 1 IHT IHT 40 40 40 40 40 40 ...
Страница 17: ...cess ID Positive Ion Mode Negative Ion Mode CAD CAD High High Low High Low High AF2 AF2 100 100 0 to 200 0 to 200 AF3 AF3 Mass Speed Dependent Mass Speed Dependent 0 to 5 0 to 5 EXB EXB Mass Speed Dependent Mass Speed Dependent 200 to 0 0 to 200 CES CES 0 0 50 to 50 50 to 50 ROS CE 30 30 Q0 ROS 5 to 130 5 to 130 MS MS MS only ...
Страница 18: ...4000 Series Parameters 18 Document Number D1000092200 B ...
