VWR 732-1359 Instruction Manual Download | Manualshive

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Instruction manual

VWR

®

PCR Thermal Cycler XT

96

/ XTender

96

EU cat. no

US cat. no

XT96

732-1359

76452-151

XT96 Gradient

732-3428

76452-153

XTender96

732-3657

76452-155

XTender96 Gradient

732-3658

76452-157

___

Version 2 Issue 17 October 2021

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Summary of Contents for 732-1359

Page 1: ...l VWR PCR Thermal Cycler XT96 XTender96 EU cat no US cat no XT96 732 1359 76452 151 XT96 Gradient 732 3428 76452 153 XTender96 732 3657 76452 155 XTender96 Gradient 732 3658 76452 157 ___ Version 2 Issue 17 October 2021 ...

Page 2: ...searchpark Haasrode 2020 Geldenaaksebaa n 464 B 3001 Leuven 3216385011 be vwr com UK Importer VWR International Ltd Hunter Boulevard Magna Park Lutterworth Leicestershire LE17 4XN uk vwr com United States VWR International LLC 100 Matsonford Rd Radnor PA 19087 1 800 932 5000 www vwr com Country of Origin GERMANY ...

Page 3: ...view Simple Mode 15 Run dialogue Overview Expert Mode 16 Program Dialogue 22 Deleting a command from the program 25 Available commands program steps 26 GLPs dialogue 28 Explorer dialogue 30 Diagnose dialogue 32 System dialogue 33 MAINTENANCE 41 Cleaning maintenance and repair of the thermal cycler 41 Returns only in original packaging 41 Temperature check up 41 Calibration 41 ORDERING INFORMATION ...

Page 4: ...mal cyclers Transfer of data can be done by using commercially available USB memory sticks enabling a nearly unlimited saving space for programs and GLP reports which can be printed using a printer connected to the thermal cycler via the network Notice The USB ports support only standard cable mice and standard USB sticks The maximum acceptable length of the connecting cable of the port is 3 m GEN...

Page 5: ...ed until the temperature reaches 30 C 86 ºF or less Only use materials tubes tube caps plates and sealing films which are heat resistant to 120 ºC 248 ºF Caution Environment The ventilation of the device must not be covered Danger Line voltage Incorrectly grounded products can be extremely hazardous in the event of a fault Use only a 3 conductor power cable 3x 0 75 mm2 with protective ground The p...

Page 6: ...peration There should be enough space to make sure that the ventilation grills on the front and back are not covered and sufficient ventilation is provided The accessibility of the power switch must not be hindered There should be a distance of at least 25 cm 10 inch to the wall or the next device Two thermal cyclers should not be placed back to back or back to front The instrument is designed for...

Page 7: ...ure optional Maximal gradient range over 8 columns respectively 30 C 15 C Temperature range Gradient 30 to 100 C Gradient accuracy 0 2 C User interface functions Touch sensitive capacitive TFT Display 800 x 480 not included in the XTender Direct help function Internal flash memory for 500 000 typical PCR programs in free configurable folders subfolders Unlimited number of programs via network PC o...

Page 8: ...8 vwr com I Instruction manual VWR PCR Cycler XT96 CONNECTIONS 1x USB interface 1x USB interface Ethernet interface AC Power inlet Status LED Main power switch ...

Page 9: ...ff and the mains plug pulled before cleaning is performed INSTALLATION OF THE PCR CYCLER XTENDER96 The VWR thermal cycler XTender96 is a fully equipped PCR Thermal cycler without a Touch screen the ideal device controlled by a PC or from another XT96 Thermal cycler To connect multiple XTender96 Thermal Cyclers you need An Ethernet cable for each XTender96 Thermal Cycler A network or an external ne...

Page 10: ...ions on your XTender96 Thermal Cycler Note Every XTender96 Thermal Cycler needs a separate XT96ConfigFile file where the instrument name is unique OPERATING THE PCR CYCLER XT96 General operation The operation of the thermal cycler can be run by the internal touch screen by using the touch sensitive surface of the display or by using a mouse connected to an available USB port Furthermore the therma...

Page 11: ...ing and cooling of samples reaction tubes must be in complete contact with the block Block must be clean Press the lid downwards till the lid locks the height of the heating lid will be adjusted automatically If the touch screen of the Thermal cycler will not be used for at least 2 minutes a group view will be shown Displayed information is Thermal cycler connected to this device status of each cy...

Page 12: ...ues are explained below Change User This dialogue is used for selecting a User The user account management user groups or single users can be configured in the System dialogue see page 11 Incubate This dialogue is used for selecting and executing the incubation mode It can be activated to manually program temperatures of the thermal plate and heated lid This is useful for fast and easy incubations...

Page 13: ...gue is also used for checking serial and version numbers of hardware and software respectively Help Button Use this button to activate deactivate the direct help mode The help function is active when the button appears pressed If the mouse pointer is visible a question mark appears alongside By touching a function button or other dialogue element the corresponding help window will appear Closing t...

Page 14: ...e into the incubation mode The temperature for the thermal plate and lid can be set manually Setting the block and lid temperature and timer Set temperatures of block and lid and time by entering the values of choice in the corresponding editor fields By entering values 35 C 104 ºF the lid heating will be turned off ...

Page 15: ...mal plate Lid Current temperature of the lid heat Run dialogue The Run dialogue is used for running and monitoring existing PCR protocols Two versions are available the Simple Mode with reduced information or the Expert Mode with access to all main functions Run dialogue Overview Simple Mode The following functions are found in the Run dialogue Expert Mode Simple Mode Stop Button Start Button Time...

Page 16: ...the Protocol Wizard can be used to create new Protocols Run dialogue Overview Expert Mode The following functions are found in the Run dialogue Protocol window Cycler selection User List view diagram view Expert Mode Simple Mode Start Info Button Select protocol Program name Recently used Protocols use pin to lock a protocol in the list Open Explorer Stop ...

Page 17: ...protocol name program options author settings and comments Start Pause Use this button to start a PCR protocol or pause a PCR protocol that is in progress Stop Use this button to stop a PCR protocol that is in progress Select Program dialogue Recent Protocols Protocol Wizard Stored programs or recently used PCR protocol can be selected and loaded Also the Protocol Wizard can be used to create new ...

Page 18: ...he primer annealing temperature Note In general the annealing temperature is about 5 C below the calculated average primer pair melting temperature Tm 4 Enter the elongation Temp 72 C recommended and time Note Elongation time s product length bases 1000 bases 60 s Press OK to create a 3 step PCR Protocol with the selected settings Press to save or to save and start the new protocol Run dialogue Op...

Page 19: ...n to view the selected program in diagram view If the path for the printer is already entered simply click for printing Using the button a test printing will be performed to check the correct settings Starting a program If a program is opened the contents of the program can be seen in the program window The loaded program is started by using the Start button A program is running General Pause and ...

Page 20: ...utton If this button is pressed after starting a program the adjoining security query appears By confirming the security query with Yes the program will be stopped Status Display In this window the following information on the status is shown during a run Cycle The number of finished cycles is shown Lid Temperature of the heated lid Time remain Remaining time of PCR program Block Average temperatu...

Page 21: ... Note The GLP report also appears when a program is finished The GLP report will be created and shown on the display It will be saved under the file name program name _ date _ time glp if not deleted by the user For this the adjoining window is shown GLP file will be saved as a default setting to change this parameter go to System Thermal Block and deselect Always save GLP s function If the GLP Re...

Page 22: ...are explained in this chapter The function buttons serve for editing of programs see following section Function buttons of the Programs dialogue Entering a command To enter a new command into a program the desired line has to be selected After selecting the program command from the list in the left window it can be inserted above the desired place via the button Alternatively the command can be in...

Page 23: ...aturation If Power Fail Denaturation will be activated the adjoining dialogue appears If a power failure happens while a program is running the thermal cycler will restart the program at the point it was stopped as soon as power is restored Auto restart after power failure Additionally if the option Power Fail Denaturation is activated the thermal block will heat to the stated temperature for the ...

Page 24: ...a program The adjoining window appears to add the program name and select the folder to save Save Run A program can be saved and run immediately using this button The adjoining window appears see saving a program to add the program name and select the folder to save Do Undo a Step Use this button to do or undo a step after adding or deleting a step into a program Editing a command The parameters o...

Page 25: ...he program Select command to be deleted and then push the Delete button A command can also be deleted directly in the selected command List view Push this button to view the selected program in list view Diagram view Push this button to view the selected program in diagram view ...

Page 26: ...ated automatically during temperature steps 30 C Temperature Use this button to insert a temperature step in the PCR program Temp C Set the required temperature for the step Time Set the required hold time for the step In hours minutes and seconds Ramp Rate Activate the checkbox if a custom ramp rate is desired for the step The setting here will overwrite the Global Program Ramp in the program opt...

Page 27: ...sired temperature for the centre of the gradient Gradient Set the desired temperature span of the gradient The resulting temperatures in the 8 rows are shown on the buttons at the left side of the window Time Set the desired duration of the gradient step in hours minutes and seconds Ramp Rate Activate the checkbox if a custom ramp rate is desired for the step The setting here will overwrite the Gl...

Page 28: ...al If the exact time of sample removal is uncertain activate the Infinite field to store for an infinite time Did you know Shorter PCR protocols and moderate temperatures 8 C 10 C in the Store Step will reduce the power consumption of your PCR Thermal cycler dramatically For saving energy and prevent excessive condensation GLPs dialogue The GLPs dialogue is used for the administration viewing and ...

Page 29: ...ored GLP report Select the desired GLP report from the file list and open it by pushing the button Open The GLP report is shown as displayed Save Use this button to save a pdf file of the selected GLP report Printer Use this button to print out the selected GLP report This window shows the content of the GLP report Shows the name of the selected GLP report The name of the GLP is composed of the na...

Page 30: ...gue is used for the data management with the thermal cycler Edit Use this button to rename marked Files and Folders Marked files and folders are shown with a blue background Delete Use this button to delete marked Files and Folders Marked files and folders are shown with a blue background ...

Page 31: ...or folders to be copied Marked programs folders are shown with a blue background Past Pushing the button allows copied programs or folders to be past The button appears in blue when a folder program is already copied Start Pushing the button will start a selected PCR program directly from the Explorer mode Only possible if no other PCR Program is running ...

Page 32: ...bation is running the thermal cycler automatically records all temperatures Operation of the Diagnostics dialogue Different operating elements for indication and navigation within a diagram are available Navigation button up and down Navigation button left right Zoom in and out vertical Zoom in and out horizontal Automatic scrolling active or inactive Display of the selected zones 8 zones per bloc...

Page 33: ... single users can be configured and their settings e g right of access reading programs edit programs etc administered The users are assigned to given or predefined user groups to which are assigned rights of access Important the thermal cycler supports the assignment of rights of access to certain user groups This means that rights for the operation of functions are given or refused to certain us...

Page 34: ...users can be a changed edit Select the user from the list and click on Edit User The adjoining dialogue window appears where appropriated group the username and password can be changed Delete User In this dialogue single users can be deleted Select the user from the list and click on Delete User The adjoining dialogue window appears to confirm the step Group Management In this dialogue Groups can ...

Page 35: ...ile name will appear in the box and can be activated by a click in the checkbox Each sound may be selected to play once standard setting or in a loop Loop When this checkbox is activated the user call will be repeated periodically as long as the event is open Play sound The selected sound can be listened to when pushing this button Sound intensity Set the desired sound intensity Time Date Using th...

Page 36: ... To disconnect a LAN connection with a cycler mark the chosen unit in the overview and use the button Connect Disconnect The cycler can then be controlled again via touch screen Alternatively the LAN connection will be stopped when the cycler is switched off or the software at the PC is closed Add Cycler Using this function a connection to a new cycler for remote control can be set up manually Ent...

Page 37: ...cler displayed in the LAN control window in our example XT96 To identify this cycler mark it in the overview and use the button Identify Cycler The status LED of the marked cycler will start blinking for 5 seconds Rename Cycler Use this function to change the cycler name displayed in the LAN control window in our example XT96 Print The XT96 thermal cyclers can print GLPs on a network printer Using...

Page 38: ...le General Parameter This function is used to change general parameters as Lid deactivation Temperature save GLP Files in general or Calibration tools settings only for service purposes Enable Gradient Enables the gradient function on a non Gradient Thermal Cycler Software key can be ordered separately 732 3482 Please ask your local VWR partner Export Logs For troubleshooting the thermal cycler ca...

Page 39: ...ings done in the System dialogue will be resetted to their default settings program and GLP files will be deleted Configure USB Stick Insert an USB Stick into the Cycler or PC Configure the network settings for the thermal cycler XT96 or XTender96 as shown in the picture Make sure each instrument name is unique and press OK A XT96ConfigFile file will be generated and stored on the USB stick Use th...

Page 40: ... thermal cycler Note For each thermal cycler a new XT96 Config File must be created Licence Info Software licence of the XT96 GUI will be shown Network Settings Using this button network settings can be changed To change settings click into the box and enter the required settings for network integration Please ask your network administrator for the required settings Service Use of the service func...

Page 41: ...e device must not be done by the user but exclusively by authorized specialists Therefore the device must be sent to the manufacturer The surface of the device should be decontaminated with a lint free cloth soaked with 70 ethanol before sending The drill holes of the thermoblock shall be cleaned with a 1 5 hypochlorite solution with the help of a cotton bud Please fill in and sign the decontamina...

Page 42: ...ill be free from defects in material and workmanship for a period of two 2 years from date of delivery If a defect is present VWR will at its option and cost repair replace or refund the purchase price of this product to the customer provided it is returned during the warranty period This warranty does not apply if the product has been damaged by accident abuse misuse or misapplication or from ord...

Page 43: ...n and recycling It s also your responsibility to decontaminate the equipment in case of biological chemical and or radiological contamination to protect from health hazards the persons involved in the disposal and recycling of the equipment For more information about where you can drop off your waste of equipment please contact your local dealer from whom you originally purchased this equipment By...

Page 44: ...2 sales ie vwr com Italy VWR International S r l Via San Giusto 85 20153 Milano MI Tel 39 02 3320311 info it vwr com Korea Avantor Performance Materials Korea Ltd 2F ACE Gwanggyo Tower I Daehak 4ro 17 Yeongtong gu Suwon Korea 16226 Tel 82 31 645 7256 saleskorea avantorsciences com The Netherlands VWR International B V Postbus 8198 1005 AD Amsterdam Tel 31 0 20 4808 400 info nl vwr com Mexico VWR I...

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