Thermo Scientific Sorvall G3 User Manual Download

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Sorvall
T3/G3

User’s Manual

Analyze

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Detect

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Measure

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Control

Summary of Contents for Sorvall G3

Page 1: ...Sorvall T3 G3 User s Manual Analyze Detect Measure ControlTM ...

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Page 3: ...Sorvall T3 G3 User s Manual T3 G3 ...

Page 4: ...on contained in this document is the property of Thermo Fisher Scientific it may not be duplicated or distribuited without the owner s authorization The validity of the guarantee is subject to the observation of the instructions and precautions described in this document ...

Page 5: ...Thermo Fisher Scientific MANUAL NUMBER REV ECR ECN DATE DESCRIPTION BY a 11290098 02 07 Initial release Preface T3 G3 i ...

Page 6: ...3428 G3 100V 50 60 Hz 75003434 RG3 230V 50 60 Hz 75003433 RG3 120V 60 Hz 75003429 RG3 100V 50 Hz 75003444 RG3 100V 60 Hz Item number Quantity Check Centrifuge 11290098 User manual 1 CE Declaration of compliance 1 Manual lid opening instructions 1 Imbalance protection instructions 1 64227001 Mains cord 1 26227005 Toolbox 11210935 Rotor removal tool 1 85241084 Emergency unlooking tool 1 86000897 12 ...

Page 7: ...ie Thermo Fisher Scientific Produkte zu identizfizieren die unter diese RoHS Anweisung fallen finden Sie unter www thermo com WEEERoHS Conformità WEEE Questo prodotto deve rispondere alla direttiva dell Unione Europea 2002 96EC in merito ai Rifiuti degli Apparecchi Elettrici ed Elettronici WEEE È marcato col seguente simbolo Thermo Fisher Scientific ha stipulato contratti con una o diverse società...

Page 8: ...C des Déchets d Equipements Electriques et Electroniques DEEE Il est marqué par le symbole suivant Thermo Fisher Scientific s est associé avec une ou plusieurs compagnies de recy clage dans chaque état membre de l union européenne et ce produit devrait être collecté ou recyclé par celles ci Davantage d informations sur la conformité de Thermo Fisher Scientific à ces directives les recy cleurs dans...

Page 9: ...Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France declaramos que el instrumento bajo descrito está fabricado según las especificaciones establecidas por las siguientes normas europeas Nós Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France declaramos que o instrumento aqui apresentado está de acordo com as normas européias abaixo listadas Noi Therm...

Page 10: ...en it leaves the factory and will replace or repair the unit if it proves defective in normal use or during service for a period of ONE YEAR from the delivery This guarantee is invalid if the unit is incorrectly used poorly serviced or neglected mis used or accidentally damaged There is no explicit guarantee other than as stated above ...

Page 11: ...0 14 00 France Tel 33 1 69 18 77 77 33 2 28 03 20 00 Belgium Tel 32 24 82 30 30 Germany Tel 49 61 84 90 69 40 Fax 49 61 03 40 81 012 Finland Tel 35 89 32 91 00 Italy Tel 39 02 95 05 91 Netherlands Tel 31 7 65 71 44 40 Russia Cis Tel 7 09 52 25 11 15 Spain and Portugal Tel 34 9 32 23 31 54 Switzerland Tel 41 1 4 54 12 12 United Kingdom and Ireland Tel 44 87 06 09 92 03 Other Europe Middle East or A...

Page 12: ...cations 2 2 Installation 3 1 Environmental Conditions 3 1 Unpacking 3 1 Positioning 3 2 Mains Supply 3 2 Lid Opening and Rotor Checking 3 3 Manual Lid unlocking Procedure 3 3 Performance and Accessories 3 5 TTH751 Swing out Rotor Cat N 11210936 3 5 Angle Rotors 3 8 Instructions for Use 4 1 Controls and Indicators 4 1 Display Screens 4 2 Control Panel Functions 4 2 Control Panel Indicators 4 3 Desc...

Page 13: ...Limitations 5 2 Aerosol Risks 5 2 Service and Maintenance 6 1 Periodic Cleaning 6 1 Contamination Hazards 6 1 Disinfection 6 2 Radioactive Decontamination 6 2 Power Supply Circuit Breaker 6 3 Rotor Removal 6 3 Trunnion Lubrication 6 3 Chemical Compatibility Table for Rotors Tubes and Accessories 6 4 Disposal of Product 7 1 Chapter 5 Chapter 6 Chapter 7 ...

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Page 15: ...ching off the main power supply Coming in contact with high voltages could cause severe injuries L In this manual this symbol means that you will find important information for safety which if unobserved could result in damage to the appliance and or harm to the operator L In this manual this symbol means that you will find important information about minimizing biological risk if unobserved the r...

Page 16: ...gerant R134a is free of CFC Chloro Fluoro Carbons in accordance with the Montreal Protocol directions for preservation of the ozone layer in the atmosphere The actual temperature in the bowl is continuously displayed on the front panel of the centrifuge Under certain conditions high speed long duration runs the sample temperature can be higher than the bowl temperature on the display In this case ...

Page 17: ...ning The lid access lamp indicates when the rotor has stopped consequently the handle on the right hand side of the centrifuge can be used to open the lid If a power failure occurs access to the samples in the centrifuge is possible via a manual lid opening procedure see appropriate paragraph For this operation a special tool supplied with the centrifuge is required Bypass the interlock system onl...

Page 18: ...uation and will not operate Relative Centrifugal Force RCF at the circumference of a rotor and bucket combination is directly proportional to the speed r p m and radius of the rotor Therefore a greater r p m and or a larger radius produces a greater RCF and improved faster separation of substances The centrifuge control system carries out and displays the results of all calculations related to spe...

Page 19: ...t packed kg lb 91 201 120 264 116 256 145 319 Centrifugation Specifications T3 G3 RT3 RG3 Max capacity Swing out 4x750 mL 3000 mL 4x750 mL 3000 mL Fixed Angle 8x100 mL 8x100 mL Max density 1200 kg m3 1200 kg m3 Max load 3 6 kg 3 6 kg Max speed Swing out 4000 rpm 4500 rpm Fixed Angle 7800 rpm 8000 rpm Max RCF Swing out 3810 x g 4822 x g Fixed Angle 9115 x g 9588 x g Max noise 66 dBA 62 dBA T3 G3 RT...

Page 20: ...emperature set display 9 C to 40 C 9 C to 60 C Acceleration rates 5 5 Braking rates 5 inertial 5 inertial Drive system direct direct Brushless induction motor yes yes Electrical specifications T3 G3 RT3 RG3 Nominal operating voltage 230V 230 V 10 230 V 10 120V 120 V 10 120 V 10 Power requirements maximum 1200 W 1500 W Heat output maximum 3750 BTU h 4435 BTU h ...

Page 21: ...er handling equipment i e fork lift trolley that complies with current regulations and by people having undergone the necessary training Thermo strongly recommends that all operators comply with the local laws and regulations on safety and health in the workplace The machine must be supported from underneath If it has to be transported without its pallet for example on a staircase professional han...

Page 22: ...rds related to protection against indirect contact the supply of power to the instrument must be via a power socket fitted with a protection device ensuring automatic cut off in the case of an insulation fault A supply fitted with a circuit breaker of the correct rating complies with this requirement Ensure that the centrifuge has been switched ON Pull the handle on the centrifuge right side towar...

Page 23: ...nd of the necessary precautions Rotating parts are a risk as they could come in contact with the user or be ejected There is particularly high risk of injury if The user attempts to manually stop the rotor Any object falls inside the centrifugation chamber while the rotor is running L Manual lid unlocking may be necessary under a very limited number of conditions such as the urgent recovery of cri...

Page 24: ...ing tool pull the lid lever toward you the lid opens upwards En cas de nécessité pour le déverrouillage manuel du couvercle attendre 10 min ou l arrêt du rotor avant d utiliser l outil spécifique fourni à cet effet Cette étiquette après avoir été percée doit être changée If it is necessary to open the lid manually wait 10 min or until the rotor has stopped rotating before using the lid opening too...

Page 25: ...s 6 85240471 Cushion 4 for 750 ml bottles 11174218 Sealed buckets 4 Inserts see table 81005750 750 ml bottles 6 85240471 Cushion 4 for 750 ml bottles 11175372 Bucket for CORNING 250 ml conical bottles 11175378 Sealed bucket for 250 ml conical bottles 11175370 Bucket for Nalgene 500 ml flat 11175371 Adapters 4 for 250 ml conical bottle in 11175370 11210823 Microplate carriers 4 11174207 Rubber cush...

Page 26: ...4208 Black orange 12 48 16 5 125 10 flat 184 4000 3291 4500 4166 11174188 Beige 12 36 18 120 10 flat 172 4000 3077 4500 3894 11174161 Brown 7 28 17 7 134 15 vac 185 4000 3309 4500 4188 11177573 Black 12 unsealed 48 18 122 15 con 188 4000 3363 4500 4256 1175781 Black 10 40 18 122 15 con 188 4000 3363 4500 4256 11174190 Orange 12 48 16 5 125 15 184 4000 3291 4500 4166 11174163 Coral 12 48 22 2 108 2...

Page 27: ...e 11175370 Bucket for Nalgene 500 ml Flat T3 G3 RT3 RG3 Cat N Color of insert Insert Rotor Max Tube Max Tube Max Max Max Max Max set of 4 or type Capacity Capacity Ø mm tube height volume Radius Speed RCF Speed RCF mm mL mm rpm x g rpm x g 11175371 1 4 250 con 208 4000 3721 4500 4709 ...

Page 28: ... 9588 x g 35 angle Cat No Insert capacity Max tube Ø mm Accessory description 64662300 1 16 5 Set of 8 adaptors for 10 mL vac tubes Angle rotor 10x50 mL tubes Ø 34mm L min max 97 115mm 8000 rpm 9302 x g 35 angle conical bottom CD 100 Angle Rotor Cat N 11210937 CD 50c Angle Rotors Cat N 11210938 ...

Page 29: ... 4 Program Keys 5 Pre Cooling 6 Speed RCF Toggle 7 Time Radius Toggle 8 Acceleration rate set 9 Deceleration rate set 10 Stop Run 11 Pulse Run 12 Start Run 13 Temperature set R only Display Screens 15 Display speed RCF 16 Display time raduis 17 Display accel decel rates temp R mod LED Lights 1a Program 1 Indicator 2a Program 2 Indicator 3a Program 3 Indicator 4a Program 4 Indicator 5a Program Prec...

Page 30: ... is displayed C During a run the actual bowl temperature is displayed C Control Panel Functions Function Keystroke sequence and description Start cycle Press the start button 12 Stop cycle immediate Press the stop button 10 Pulse Press the pulse button 11 and hold The centrifuge will accelerate the rotor to the speed setting of the active program When the button is released the rotor will be decel...

Page 31: ...lly saved in memory To lock a program To lock a program press and hold the corresponding button for several seconds until the correspon ding LED begins to flash Release the button and the program is locked Parameters of the program cannot be changed To unlock a program To unlock a program when a program is locked the associated LED flashes press and hold the corresponding button for several second...

Page 32: ...e selected program is not locked Set speed can be changed by selecting speed as the control parameter for the upper display then by pushing the up or down arrow or turning the knob Set run time can be changed by pressing the up or down arrow corresponding to the middle display Set deceleration profile can be changed by first selecting the deceleration profile pushing the B button and then using th...

Page 33: ...ower switch on Press any keyboard button to erase this message Note if Error 01 has been detected and not cleared as above described the alarm reset is possible only after a safety time of 30 minutes Wait for rotor to stop Press any keyboard button to erase this message Press any keyboard button to erase this message Close the lid and press START Wait for the centrifuge to stop Press any keyboard ...

Page 34: ...for the safety of the appliance and of the operators L Normal use of the centrifuge could require the manipulation of biohazards Users and service personnel must have specific training for each substance they use according to the Laboratory Biosafety Manual from the World Health Organization L The contents of each rotor pocket including sample tube cap and adapter where used must be of the same we...

Page 35: ... Dynamic balancing consists of placing the tubes in each insert symmetrically according to the axis of the trunnions If less than the maximum complement of samples is loaded the tubes must be placed in opposite pockets An odd number of tubes requires an additional blank water filled tube of identical total weight to balance Significant vibrations can damage the accessories the unit and the samples...

Page 36: ... the centrifuge and redistribute the samples to produce an equal weight on diametrically opposite sides Also remember that the pins must be softly lubricated Inadaquate lubrication of the pins may cause incorrect swinging of the buckets resulting in imbalance phenomen See chapter 6 6 for details If the message persists despite your efforts to balance the load call your Thermo Service representativ...

Page 37: ...he persons operating the centrifuge This switch has to be remote from the laboratory centrifuge preferably outside the room in which the centrifuge is located or adjacent to the exit from that room Moving or shifting the machine during centrifugation is dangerous Using the centrifuge with rotating equipment showing evidence of corrosion and wear marks manipulating and or tampering with the electro...

Page 38: ...tor RCF performance displayed is the maximum available in the rotor pockets Depending on the sample containers or on other application needs the maximum RCF which must be used is to be determined by the operator The centrifuge is not explosion proof Using this centrifuge with explosive samples is entirely at the user s own risk Do not under any circumstances use the centrifuge in an explosive envi...

Page 39: ...herefore follow the instructions of the tube supplier concerning the fill level and sealing of the tube Once a biological risk is known or suspected samples should be placed in a sealed container Should such containers not be available sealed accessories should be used such as angle rotors with sealed lids When aerosols are created in a centrifuge bowl they persist in the air for a period of 3 5 m...

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Page 41: ...ockets are well dried Ideally spray with Anti Corrosion Spray Cat N 11175399 Store unused buckets upside down on a non metallic grid to allow free passage of air The centrifuge is likely to be used in medical research where hazardous substances including radioactive chemicals are frequently found ALWAYS USE THE APPROPRIATE DECONTAMINATION PROCEDURES WHEN THE ROTOR IS EXPOSED TO THESE CHEMICALS L E...

Page 42: ...t should never be used if there is surface damage to the rotor Formalin 37 formaldehyde in water in contact for 10 minutes has a similar effect to chlorine bleaches Rotors should be thoroughly rinsed under running water for 5 minutes to remove all traces of formalin then dried completely Formaldehyde is toxic L Gluteraldehyde 2 sold under many brand names such as Cidex and Glutarex requires total ...

Page 43: ...he trunnions regularly with a dry wipe as well as the part of the bucket that rotates on the trunnions Then put a very small quantity of grease on the curved face of the trunnion Do not apply too much grease because it will eventually coat the bowl of the centrifuge as centrifugal force pulls the grease from the trunnions If the centrifuge is having imbalance problems try this operation before cal...

Page 44: ...S M U S S M S U Acetonitrile O O O O O O O O Mt U S Mt U O O O O O Acetylene S O O O O O O O S O S S U O O O O O Adipic Acid O O O O O O O O S S St S S O O O O O Alanine S O O O O O O O S S U S U O O O O O Allyl Alcohol O O U O S O O U O S S S O O S S S O Aluminum Chloride O O S S O S O S S S S S O U S S S O Aluminum Fluoride O O O O O S O S S U S S O O S O S O Aluminum Hydroxide S O O O O O O O S...

Page 45: ...O O O O S S S S S O S S O O Caesium Chloride M O S S O O O O S S S S S S S S S O Caesium Formiate M O O O O O O O S S S S S O S S O O Caesium Iodide M O O O O O O O S S S S S O S S O O Caesium Sulphate M O O O O O O O S S S S S O S S O O Caesium Trifluoracetate M O M U O O O O S S S S S M S O O O Calcium Carbonate U O O O O O O O S O St S O O O O O O Calcium Chloride M S S O S O O S S M O D S S S ...

Page 46: ... S M S O Dimethylsulphoxide S O O O O O O O S U O S O S S M S O Dioxane S U U O M S O O M U M M O O S S S U Diphenyloxide S O O O O O O O U O O U O O O O O O Dipropylene Glycol O O O O O O O O S Sd S S S O O O O O Distilled Water S S S S S O S S S S S S S S S S S S Ethanol 50 S S S S M S S U S M S S S S S S S S Ethanol 95 S S U U M S S U S U S S S S S S S S Ethyl Acetate M U U U M S O U M U S U U ...

Page 47: ...S U M Iodine Crystals S O O O O O O O Mt U U Mt U O O O O O Isobutyl Alcohol O M U O S O O U S S S S O O S S S S Isopropyl Alcohol U M U U S O S U S M S S M O S S S S Isopropylbenzene O O O O O O O O Mt U Mt Mt U O O O O O Kerosene S O O O O O O O Sd S Mt Sd St O O O O O Lactic Acid 20 O S O O O O S O S S S S S S S S S S Lactic Acid 100 O S O O O O O O S S S S O S S S S S Lead Acetate aq U O O O O...

Page 48: ...chloric Acid 70 X O O O O O O O M U M M U O S S O O Petrol S O O O O O O O St M St St M O O O O O Phenol 5 S U O O U S O U M U S M U S S S U S Phenol 50 U O O O O O O O U U U U U O S M O O Phenol crystals U O O O O O O O St U St St U O O O O O Phenol liquid U O O O O O O O U U U U U O O O O O Phenyl Ethyl Alcohol O O U O O O O S S O S S O O S O S O Phosphoric Acid 10 O M S S U S S O S S S S S S S ...

Page 49: ... O S S S M S Stearic Acid S O O O O O O O S Sd S S S O O O O O Sulphuric Acid 10 M U S S U S S S S M S S S U S S S S Sulphuric Acid 20 U O O O O O O O Sd Sd S Sd S O O O O O Sulphuric Acid 50 U U U U U S S U S S S S S U S S M S Sulphuric Acid conc U U U U U S O U D U M D U M S S U S Sulphuric Anhydride dry or moist S O O O O O O O S Sd S S S O O O O O Tetrachloroethane M O O O O O O O M O O M O O ...

Page 50: ... Hydrosulphite U O O O O O O O O O O O O O O O O O Zinc Sulphate U O O O O O O O Sd O S Sd O O O O O O This table is intended as a guide only because of the difficulty in quantifying cross checking and monitoring the results under different conditions of temperature pressure and purity relating to the solvents and samples dissolved therein It is strongly recommended that you carry out your own tri...

Page 51: ...he company or persons who are in charge of disposal The elements of the product that may become noxius during the life of the product are the folowing Electric batteries and accumulators situated in the front panel of the machine among the other electrical and electronic componenets Oil in the compressor of the refrigeration systems Refrigerant Disposal of these elements must be in accordance with...

Page 52: ...f the following Product type Serial N Rotor Serial N Rotor Serial N Accessory Description Serial N Accessory Description Serial N Nature of contamination Decontamination Procedure used Decontamination certified by Mr Mrs Institution Date Signature When an instrument or parts of it requires servicing by Thermo personnel the following procedure must be accomplished to ensure personnel safety Clean t...

Page 53: ... our opinion is a radioactive or biological hazard the item will be refused and resent to the Customer Disposition costs will be borne by the sender Instructions for decontamination and cleaning are explained in the User Manual Additional certificates are available from your local technical or Customer Service representative In the event these certificates are not available a written statement cer...

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Thermo Scientific Sorvall G3, User Manual

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