UC BSD Light Microscopy Core Facility Vytas Bindokas, Director
Olympus IX70 Multi-parameter Fluorescence Microscope
Operations Manual (version 1.0 February ‘09)
The IX70 microscope is a manual stand with the ability to collect multi-color fluorescence, differential
interference contrast (DIC) and total internal reflection fluorescence (TIRF), as well as provide optical
sectioning (z-axis stepping) and time lapse imaging. It is equipped with a high sensitivity, high resolution
chilled CCD camera, a green/red or blue/red emission beam splitter for simultaneous two color capture
and Met MetaFluor software to control the system.
Examples of types of images that can be captured range from simple image capture (bright field or
fluorescence), sequential capture of up to 4 fluorescent probes, time lapse imaging, (relatively) high speed
imaging, CFP-YFP FRET studies, ratiometric indicator dyes such the calcium probe Fura-2, etc.
Software provides a wide range of image capture and image processing capabilities. MetaFluor is
optimized for real-time (ratio) image capture with real time region of interest (ROI) intensity/
ratio/calibrated data display. MetaMorph is a more general image capture, processing, morphometry
package. Both packages allow images to be automatically collected and annotated.
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