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NeoFox Phase Fluorometer 
Installation and Operation Manual 

For Products: NeoFox-GT and NeoFox Sport 
Document: 013-20000-009-02-201510 

Summary of Contents for NeoFox

Page 1: ...NeoFox Phase Fluorometer Installation and Operation Manual For Products NeoFox GT and NeoFox Sport Document 013 20000 009 02 201510 ...

Page 2: ...roperty of their respective owners Limit of Liability Every effort has been made to make this manual as complete and as accurate as possible but no warranty or fitness is implied The information provided is on an as is basis Ocean Optics Inc shall have neither liability nor responsibility to any person or entity with respect to any loss or damages arising from the information contained in this man...

Page 3: ... not look directly at the LED output with the naked eye once the SMA cap is removed To verify there is power to the instrument place a white piece of paper in front of LED 1 2 Do not open the instrument case High voltage is present There are no serviceable parts inside Return the instrument to the factory for service ...

Page 4: ... Oxygen Sensor Probes 2 Software 3 Applications 3 Shipment Components 3 NeoFox 3 NeoFox Sport 4 Additional Recommended Equipment 4 Chapter 2 Installation 5 Overview 5 Software Installation 5 Hardware Installation 8 Installing NeoFox Hardware Drivers 10 Installing Hardware Drivers Using Windows Vista 10 Installing Hardware Drivers Using Windows XP 12 Chapter 3 Using NeoFox with NeoFox Viewer Softwa...

Page 5: ...port Menu 34 Setting Up the NeoFox Sport 36 Data Logging Saving Data 37 Logging Data 37 Viewing the Data Log File Name 38 Setting the Data Logging Interval 38 Data Log File Format 38 NeoFox Sport Saved Settings 39 Low Battery Detector 39 Appendix A Specifications 41 Appendix B Maintenance 43 Updating Firmware 43 Updating Software 43 Troubleshooting 43 Appendix C Analog Output 47 Analog Output Conn...

Page 6: ...log Output Pinouts 51 Analog Output Parameters List 51 Analog Output Specifications 52 Interfacing to the 4 20mA Output 52 4 20 mA Loop Voltage Supply 53 4 20 mA Isolation 54 Resistor Volt Meter 54 Appendix D Calculating Drift Per Sensor 57 Index 59 ...

Page 7: ...ns Chapter 3 Using NeoFox Contains steps to operate NeoFox Viewer software with the NeoFox hardware Also contains steps for restoring screen defaults Chapter 4 Using NeoFox Sport Contains the menu structure and operational information for the NeoFox Sport Appendix A Specifications Lists NeoFox specifications Appendix B Maintenance Contains information for updating firmware and software and a troub...

Page 8: ...ironment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which the user will be required to correct the interference at his own expense WARNING The authority t...

Page 9: ...ecause the unit is self contained it is invariant to fiber bending and stray light and has a wide dynamic range of optical intensity as well as low optical and electronic crosstalk and low drift and phase noise Therefore the NeoFox is especially useful for oxygen sensing applications where stability and sensitivity to drift is important and where sample set ups must be left undisturbed for long pe...

Page 10: ...xygen partial pressure in Torr Dissolved oxygen partial pressure in ppm parts per million Dissolved oxygen concentration in um L micromoles per liter Data logging of temperature and oxygen readings in units listed above In field single point reset also available on desktop NeoFox Oxygen Sensor Probes The NeoFox is used with Ocean Optics NeoFox software and custom probes priced separately Ocean Opt...

Page 11: ...o 100 kHz The on board pressure transducer measures atmospheric pressure Software The NeoFox standalone module uses NeoFox Viewer software to display oxygen measurements on a connected Windows XP PC The NeoFox Sport can also use NeoFox Viewer software when connected to a Windows XP PC or it can be untethered from a computer and display some Oxygen measurements on its OLED screen See Chapter 4 Usin...

Page 12: ...eoFox Sport module s See your Jaz manual for more information about what ships with Jaz refer to Product Related Documentation SD card containing the neofox cal calibration file NeoFox CD Packing List Additional Recommended Equipment NeoFox Viewer Software Temperature Probe FOXY HIOXY or FOSPOR Oxygen Sensor Probe RedEye Patches FOXY HIOXY and FOSPOR FOXY CAL HIOXY CAL FOSPOR CAL This is an option...

Page 13: ... software from either the CD that came with your NeoFox unit or from the Ocean Optics website Use the following procedure to install NeoFox Viewer software on a Windows XP PC The process on Windows Vista machines is very similar The important thing to note is to make sure to install the driver from the specific location within the NeoFox Viewer USB Driver directory Note You may be prompted to inst...

Page 14: ... installer for Vista computers The installation wizard appears Installing from the Web d Go to http www oceanoptics com technical softwaredownloads asp e Right click on NeoFox Viewer Software and select Save Target As to download the executable to your machine f Click Save The installation begins g Click Run The NeoFox Viewer Setup screen appears 3 Click Next The Select Installation Folder screen ...

Page 15: ...on or browse to the desired location and click Next You are now ready to install the software 5 Click Install The Neofox Viewer installs at the selected location This process can take several minutes When the software has successfully installed the final Install Wizard screen appears ...

Page 16: ... Installation Hardware Installation WARNING Ensure that the plastic caps are covering the two SMA connectors on the front of the NeoFox unit Intense UV radiation is emitted from the LEDS when the unit is powered up Do NOT look directly at the LED output with the naked eye ...

Page 17: ...battery or provide power to the DPU Very few users will ever need to use the NeoFox module s auxiliary power connector 3 Connect the NeoFox unit to your computer using the USB cable See Installing NeoFox Hardware Drivers Note On the NeoFox Sport hardware there is a difference between the USB connector on the DPU module and the USB connector on the NeoFox module The USB connector on the NeoFox modu...

Page 18: ...hing Installing NeoFox Hardware Drivers When you connect NeoFox to a computer for the first time using the USB cable the hardware drivers should install automatically for Windows 7 8 8 1 and 10 if you installed the NeoFox Viewer software first If you are using a Windows Vista or Windows XP computer follow the appropriate instructions below to install the drivers Installing Hardware Drivers Using W...

Page 19: ...sc Show me other options The next hardware wizard screen appears 4 Select Browse my computer for driver software advanced The next hardware wizard screen appears 5 Highlight the driver_support directory located beneath C Program Files Ocean Optics NeoFox Viewer and click OK followed by Next The next hardware wizard screen appears 6 If the following screen appears select Install this driver anyway ...

Page 20: ...ers Using Windows XP The following Found New Hardware wizard appears when you first attach NeoFox to computers running the XP operating system Procedure 1 From the first hardware wizard screen select No not this time Click Next The next hardware wizard screen appears ...

Page 21: ...the software Automatically Recommended Click Next The system installs the necessary driver software to operate your NeoFox device The next hardware wizard screen appears 3 When the hardware driver installation completes successfully the following screen appears ...

Page 22: ...2 Installation 14 013 20000 009 02 201510 ...

Page 23: ...basic NeoFox Viewer screen appears This screen s controls enable you to perform the following actions Insert comments into the exported data file using the Enter New Event textbox See Entering a New Event for more information Set scans to average Select the sensor type Select Automatic or Manual Gain Start Stop Data Logging see Data Logging ...

Page 24: ...t data file your comment s will appear on the row of data that most closely corresponds to the time you created your comment Procedure To enter a new comment 1 Click the Enter New Event field at the top of the screen 2 Enter your message into the text field 3 Press the Enter key Data Logging You can save a csv file useful for Microsoft Excel via data logging When you click the Start Stop Data Logg...

Page 25: ...f the following data types All Columns Exports all available types of data Standard Exports a limited subset of the available data types O2 Tau temperature pressure Close Window Without Saving Changes Closes this window None of the changes that you have made will take effect or be saved Save Settings and Close Window All changes that you have made on this window are saved If you have clicked Start...

Page 26: ...oftware versions are not compatible You will then need to install the appropriate software version onto your computer to use NeoFox See Software Installation and Appendix B Maintenance for more information The Advanced Settings window contains the following tabs bottom right corner of screen Setup Status Advanced Status Advanced Settings Setup Setup allows you to perform such functions as Sensor T...

Page 27: ...3 Using NeoFox with NeoFox Viewer Software 013 20000 009 02 201510 19 Status Clicking on the Status tab displays the status information shown below ...

Page 28: ...510 Advanced Status The Advanced Status tab displays the values of parameters such as Oxygen base and converted Tau etc as shown below Advanced Settings This tab displays the advanced settings shown below for sensor waveform decay waveform FFT and salinity correction ...

Page 29: ... the capability to change the salinity correction setting from its default setting of 0 Salinity correction applies to dissolved oxygen for PPM and µM L it does not apply to percentage Procedure To change the salinity setting 1 Double click on the Salinity Calibration value default is 0 2 Type the new salinity value 3 Press Enter ...

Page 30: ...he APD detector which determines the detector s sensitivity towards converting photons to electrical signal This can be manually adjusted for firmware versions below 4 0 if the signal level of the sensor appears to be low For firmware versions 4 00 or above this parameter is set at the factory and cannot be changed Procedure To manually adjust the signal level 1 From the Basic View screen select V...

Page 31: ... the NeoFox hardware unit Therefore a new calibration must be loaded each time a new sensor is added or before first use You have the option of purchasing a factory calibration for your Oxygen Sensor Probe FOXY CAL HIOXY CAL FOSPOR CAL If you have a factory calibration follow the instructions for Multipoint Calibration If you do not have a factory calibration follow the instructions for a Two Poin...

Page 32: ...NeoFox Temperature Sensor 5 Under Enter Sensor Type And Optional Data choose the type of sensor and add the serial number and any notes needed Note Normalized decay time τ0 τ 6 Click the button then browse to the location of the factory calibration file on the NeoFox CD 7 Select the Multi Point Calibration tab The uploaded calibration table appears on the left side of the screen The curve fitted g...

Page 33: ...creen 9 Click the button to use the coefficients for oxygen calculation A Download Confirmation screen appears 10 Click Yes to confirm the download and continue Two Point Calibration If you want to do your own calibration instead of using a factory calibration follow these Two Point Calibration instructions Procedure 1 From the Main screen select Options Calibration 2 Click the Setup tab The Setup...

Page 34: ...er Select How Temperature Will Be Measured choose None Two point calibration only 5 Under Enter Sensor Type And Optional Data choose the type of sensor and add the serial number and any notes needed 6 Select the Two Point Calibration tab You need two known concentrations of oxygen to perform this calibration one being the 0 oxygen concentration ...

Page 35: ...conditions in air which is 20 9 oxygen 10 Enter the known oxygen value in the O2 box for point 2 11 Once the Tau value is stable click on the Use Current Tau for Point 2 box 12 Now that you have the two points of calibration click anywhere in the graph chart displayed on this screen The two points will appear on the graph circled in red on the figure above which will Compute Constants m and b 13 T...

Page 36: ...ved Settings The following parameters are saved onboard the NeoFox in its non volatile memory When the settings are saved the saved values will be loaded when the device turns on Measurement Parameters Duty Cycle Seconds on Duty Cycle Seconds off Number of Averages Oxygen Units Salinity Correction Factor ...

Page 37: ...ipoint Original Calibration Coefficients A0 A1 A2 B0 B1 B2 C0 C1 C2 T0 T1 T2 12 Single Point Adjusted Calibration Coefficients A0 A1 A2 B0 B1 B2 C0 C1 C2 T0 T1 T2 Analog Output Settings Analog Voltage Output Source Analog Voltage Lower Bound Analog Voltage Upper Bound Analog Current Output Source Analog Current Lower Bound Analog Current Upper bound Jaz Communication Enable Jaz Interface Serial an...

Page 38: ...o save the measurement data in a fxy file 1 Select File Save A window appears displaying a file name with the date and the time the file was last saved 2 Select the desired location to save the file Procedure To save the measurement data in a csv file 1 Click the Start Stop Data Logging button from the main screen The Data Logging Control Panel screen appears 2 Click the Start Logging button 3 Sel...

Page 39: ...ng Restoring Chart Layouts NeoFox Viewer allows you to save and restore the layout of the displayed charts The following settings can be saved and then restored X axis duration Vertical height settings Y axis scale settings Type s of data being displayed on multiple plotting areas within the chart window Saving a Chart Layout Procedure 1 Select View Save Graph Layout from the menu 2 Specify the na...

Page 40: ...et or manually prompting a flash write by setting the Flashed Write parameter to Enabled in the Advanced Settings Panel Resetting Saved Settings You can reset the saved settings to their original state Procedure 1 Open the device in the NeoFox Viewer and navigate to the Advanced Settings screen 2 Set the value of the Flash Reset parameter to Enabled This value may revert to disabled shortly therea...

Page 41: ...lled through the NeoFox Viewer software In fact the user interface for the desktop NeoFox and the NeoFox Sport are exactly the same This chapter contains information for using NeoFox Sport without the NeoFox Viewer software NeoFox Sport Features Additional features that are available to NeoFox Sport users include Battery powered operation Real time display of temperature and oxygen in various meas...

Page 42: ...4 Using NeoFox Sport 34 013 20000 009 02 201510 NeoFox Sport Menu Menu Tree Description ...

Page 43: ...0 50 0000 A message displays informing you that the single point reset has completed NOTE NeoFox must be configured to Single Point mode through NeoFox Viewer Data Logging Allows you to enable datalogging to an SD card set the datalogging interval and see the filename used for the stored data Default current datalog interval Range 1000 99 999 999 ms Oxygen Units Enables you to set the units in whi...

Page 44: ...Connect the power supply to the DPU power jack not the NeoFox auxiliary power jack 2 Press the red power button to turn the device on 3 Connect the USB cable between the USB port on the NeoFox module not the DPU and the computer 4 Start the NeoFox Viewer software See Chapter 3 Using NeoFox with NeoFox Viewer Software 5 Perform a calibration See Calibrating the Probe 6 Select the sensor type as app...

Page 45: ...n 4 Scroll down to the Enable Jaz Interface parameter and set it to Enabled 5 Set the Flash Write parameter to Enabled It will remain displayed as Enabled briefly before reverting to Disabled Data Logging Saving Data NeoFox Sport provides you with the following functionality for logging saving data Log data to an SD card View the file name of the data log Set the data logging interval Logging Data...

Page 46: ... single sample in the data log is formatted as follows millisecond count tab upload date tab upload time tab oxygen tab oxygen unit label tab algorithm temperature tab sensor temperature tab tau tab algorithm pressure tab sensor pressure Where Millisecond Count The millisecond counter of the NeoFox device at the time of the actual sample Because this value is stored at the actual sampling time thi...

Page 47: ...d Settings for a list of settings that can be saved To save the flash write settings navigate to the Sensor Setup Save Settings menu Note that this saves ALL of the savable parameters in their current state Also performing a Single Point Reset through the DPU menu may result in all of the savable parameters being overwritten Low Battery Detector The NeoFox Sport contains a low battery detector tha...

Page 48: ...4 Using NeoFox Sport 40 013 20000 009 02 201510 ...

Page 49: ...s digitally sampled Thermistor Probes Closed end stainless steel tube with thermistor sensor mounted in tip liquid immersible rugged design 1 8 NPT fitting temperature range 0 to 75 C Pressure Measurement On board pressure transducer monitors atmospheric pressure Input Power Approximately 2 5W 5V Communications USB 0 5V analog output 4 20mA analog output RS232 is available on the NeoFox GT model w...

Page 50: ...A Specifications 42 013 20000 009 02 201510 ...

Page 51: ...loaded Updating Software You can overwrite the previous installation Procedure 1 Download the installer for the latest version of the Neofox Viewer from the Ocean Optics Software Downloads page www oceanoptics com technical softwaredownloads asp 2 Unzip the directory and then run the Setup msi installer wizard Troubleshooting Problem Possible Cause Suggested Solution Clicking Download during a Two...

Page 52: ...y assume that any strings passed are already initialized Therefore be sure to pass only initialized strings Better error handling for this error was added in DLL version 1 89 but you should still use this convention to avoid unnecessary complications After setting the display units to anything other than ppm the display will not return to percent oxygen Previous software version This problem was r...

Page 53: ...brations will also produce incorrect results If your HIOXY probe was calibrated before 01 SEPT 2009 return it to Ocean Optics for a full recalibration at 45 KHz Or you can use firmware version 2 02 available from Ocean Optics Or you can enter the frequency setting manually although this is not recommended The temperature probe reading appears erratic or is drastically incorrect Known issue with un...

Page 54: ...B Maintenance 46 013 20000 009 02 201510 ...

Page 55: ...ailable on the NeoFox as follows 4 20 mA current source 0 5V voltage source Analog threshold settings can be saved by the user to allow a NeoFox unit to operate in a stand alone mode outputting analog signals without input from a computer Configuring Analog Voltage Output Channel with NeoFox Viewer Procedure 1 Select the Advanced Settings tab on the Advanced View screen to view the advanced settin...

Page 56: ...Limit Minimum For more information on selecting a lower bound for your voltage see Selecting a Data Range 4 Select a valid upper bound for your represented data range by typing a number into the box located to the right of the Analog Voltage Upper Bound setting In some versions of the Viewer software this setting may be called Voltage Limit Maximum For more information on selecting an upper bound ...

Page 57: ...or more information on selecting a lower bound for your voltage see Selecting a Data Range 4 Select a valid upper bound for your represented data range by typing a number into the box located to the right of the Analog Current Upper Bound setting In some versions of the Viewer software this setting may be called Current Limit Maximum For more information on selecting a upper bound for your voltage...

Page 58: ...nd represents the value of the data source that corresponds to a 0 volt analog output The analog voltage upper bound represents the value of the data source that corresponds to a 5 volt analog output Intermediate values are linearly scaled across this region to produce the appropriate output Values of the data source that fall outside this region result in output voltages that are either 0 volts o...

Page 59: ...s To save the analog output settings open the Advanced Settings control panel and then select Enabled from the dropdown box located to the right of the Flash Write setting This box will momentarily continue to display enabled but will switch back to disabled when the unit has processed the command Analog Output Pinouts Caution Be careful when wiring Incorrect wiring could cause permanent damage to...

Page 60: ...d or Current Limit Minimum floating point value This floating point value represents the lower bound of the represented range of the input data Must be UBOUND and 0 Analog Current Upper Bound or Current Limit Maximum floating point value This floating point value represents the upper bound of the represented range of the input data Must be L Analog Output Specifications Name Min Typ Max Conditions...

Page 61: ...pters to power their NeoFox unit and their interface circuitry Usually this will result in the two circuits floating with respect to each other so that the circuit will work properly However it is important to note that NeoFox has a grounded metal case that is connected to its circuit ground and its USB shell Hence customers who connect the NeoFox s case ground to their interface circuit s case gr...

Page 62: ...t Number 931S A1A1N IP1 931S C1A2D OP Manufacturer Rockwell Automation Suggested Vendor Varies by geographic region Part Number 931S C1A2D OP This part can be used to convert the 0 5V output of the NeoFox into a 4 20mA signal Resistor Volt Meter If an ammeter is not available as is the case with many data acquisition systems a voltmeter can be used instead see following figure To use a voltage mea...

Page 63: ...C Analog Output 013 20000 009 02 201510 55 ...

Page 64: ...C Analog Output 56 013 20000 009 02 201510 ...

Page 65: ...ec HR Oxygen Stability 0 01 HR 0 005 HR 0 007 HR Example Calculate drift for a FOXY sensor probe while measuring a sample every 2 5 hours for 100 hours with the LED being lit for 1 minute New drift reported drift duty cycle on duty cycle off New drift 0 01 hr 1 min 2 5 hr 60 min hr 2 5 60 converts hours into minutes so as to cancel out the dimensions New drift 0 01 hr 1 min 150 min New drift 0 01 ...

Page 66: ...D Calculating Drift Per Sensor 58 013 20000 009 02 201510 ...

Page 67: ... B battery detector 39 C calibration 4 multipoint 23 probe 23 two point 25 chart layout restore 31 save 31 Communication Interfaces 31 D data saving 30 data log file format 38 data logging 16 NeoFox Sport 37 data range selecting 50 data source selecting 49 document audience iv purpose iv summary iv E enter new event 15 Enter New Event field 16 equipment included 3 recommended 4 event entering new ...

Page 68: ... 16 O oxygen sensor probes 2 4 P probe calibrating 23 oxygen sensor 2 4 temperature 4 product related documentation iv R recommended equipment 4 RedEye 4 restore chart layout 31 S safety warnings i salinity correction 21 save chart layout 31 saved settings 28 NeoFox Sport 39 sensor oxygen 2 4 select type 22 setup NeoFox Sport 36 Setup tab 18 shipment components 3 software 3 5 17 NeoFox Viewer 3 up...

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