Cairn OptoSplit II Instruction Manual Download | Manualshive

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www.cairn-research.co.uk

Free Phone: 08453301267 (UK only)

Tel: +44 (0) 1795590140

Fax: +44 (0) 1795594510

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Summary of Contents for OptoSplit II

Page 1: ...www cairn research co uk Free Phone 08453301267 UK only Tel 44 0 1795590140 Fax 44 0 1795594510 ...

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Page 4: ...rt department who are always willing to help e mail tech cairn research co uk Note Please do not adjust any control before reading this manual The Optosplit is supplied pre aligned and centred please refer to this manual before making any adjustments When changing the Cairn Filter Cube care should be taken not to touch any optical surfaces If any lenses filters or mirrors are marked then they shou...

Page 5: ...nt Mounts Installing the Optosplit II in the Lightpath Operation of the Optosplit II Adjusting and Locking the Aperture Adjusting the Position of the Images Focussing the Camera on the Aperture Centering Images on the Camera Image Optimisation Trim Adjustment Single Channel non split mode Technical Summary Technical support 1 2 2 1 2 2 2 3 2 4 2 5 3 3 1 3 2 3 3 3 4 3 5 3 6 4 5 1 2 2 3 3 5 5 6 7 7 ...

Page 6: ...lit II allows the researcher to do exactly this The Cairn Optosplit II is usually supplied with a rectangular aperture to define a ROI and includes controls to allow the two images to be positioned accurately and conveniently within the camera frame Images can be acquired using any imaging software and processed either manually off line or using an appropriate analysis tool such as the Splitview m...

Page 7: ...g the tool provided To fit a filter remove the locking ring and place the filter orientated so that the arrow points into the Cairn Filter Cube and towards the light path Then simply replace and tighten the locking ring In most applications the dichroic beamsplitter will have longpass char acteristics so the longer wavelength of the two filters should be located in the straight through filter posi...

Page 8: ...osplit II The Cairn filter cube is designed to be easily accessible in order to facilitate quick and easy changes of filter sets Access to the filter cube mount is gained by removing the access panel using the two handles to pull gently and firmly The filter cube mount will now be visible The mount attached to the rear of the Cairn Filter Cube is designed to mate with the bracket on the internal w...

Page 9: ...Remove the access panel using the two handles Mounting bracket Replace the access panel using the two handles Mount the filter cube onto the brackect on the interior of the Optosplit II Page 4 ...

Page 10: ...for the installed filter set Failing this a standard bright field image can be used but this may lead to arbitrary intensity differences between the spectrally resolved images Firstly the CCD camera should be mounted on the microscope C mount output and the port adjusted to give the sharpest possible image Once a clear image can be seen the camera should be switched off and removed from the micros...

Page 11: ...y rotation or slant in either direction During manufacture the Optosplit II is calibrated with the two images approximately super imposed so when it is first installed a single central image is seen on screen 3 Operation of the Optosplit II The Optosplit II uses a single control for adjusting image seperation and allows for different sizes of samples to be used There are additional controls for re...

Page 12: ... of the Images Once the camera is set up it is generally a good idea to familiarise yourself with the operation of the alignment adjustments Please note that the diagrams overleaf indicate the images for a system using an appropriately aligned and centred rectangular diaphragm An image similar to that shown opposite will be observed when the Optosplit II is first used with the two images being dis...

Page 13: ...o images are located as closely as possible on the camera chip Turning the Split control anti clockwise will seperate the two images along the horizontal axis Only small adjustments will be required when using the horizontal image seperation adjustment Should the left and right images be at different vertical heights then adjust the images using the V1 and V2 adjuster Turning V1 will alter the pos...

Page 14: ...t control 4 Slightly loosen the hex screws on the focussing ring Adjust the focus whilst ensuring the optosplit is secure and holding the camera until the apertures edges are in sharp focus 5 Then lock off the focussing ring by re tightening the hex screws Ensure the camera is again lined up with its top orientated the same way as the top of the Optosplit II and then lock off the output rotating r...

Page 15: ...ked V1 on the Optosplit II body 3 The short wavelength is now correctly positioned 4 To centre the long wavelength pathway we superimpose the longer wavelength image on the shorter wavelength image we have just centred Do this using the V2 and H2 adjusters 5 Centre the image horizontally by smaller adjustments of H2 Once the image is exactly superimposed on the long wavelength image in the centre ...

Page 16: ...ved too far then the vignetting will become apparent on the other image and will need to be adjusted in the opposite direction until both images are the same intensity It is important to remember to re tighten the clamp screw once the adjustment is complete If the Optosplit II has been configured into single channel non split mode see section 3 6 andTrim adjustment is required to compensate for vi...

Page 17: ...see section 3 5 B Blocking the longer wavelength path Suitable if only the shortest wavelength channel is required 1 Leave the dichroic mirror cube in place and block the long wavelength path using the shutter plate provided Most dichroic mirror have long pass characteristics in which case the longest wavelength will be the transmitted channel See section 4 for help differentiating the channels 2 ...

Page 18: ...he degree of separation whilst maintaining identical path lengths A half size fully silvered mirror at the output port for recombining the images Fine adjustment controls for camera focus and vertical alignment Optional ROI definition using adjustabe rectangular aperture The system is supplied with high grade AR coated achromatic doublet lenses and dielectrically coated mirrors for maximum through...

Page 19: ...OPTOSPLIT II www cairn research co uk 261mm 110mm 60mm 79mm 55mm 52mm TRIM 121mm 261mm 110mm 60mm 110mm 52mm Page 14 ...

Page 20: ...chnical Support E mail tech cairn research co uk Web http www cairn research co uk Address Cairn Research Graveney Road Faversham Kent ME13 8UP Telephone 44 0 1795590140 Fax 44 0 1795594510 Page 15 Page 15 ...

Page 21: ...In addition to designing and manufacturing our own products we are also proud distributors for the following companies ...

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