Zymo Research ZymoBIOMICS D4300 Instruction Manual Download Page 1

 

For Research Use Only 

 

 

 

 

 

 

 

 

 

 

 

     Ver. 1.4.1 

 

90 

 

 

 

 
 

 

 
 

 

ZymoBIOMICS

 DNA Miniprep Kit

 

Catalog Nos. 

D4300T, D4300, & D4304

 

 

Highlights 

 

 

Validated Unbiased for Microbiome Measurements:

 Unbiased cellular lysis validated using the 

ZymoBIOMICS Microbial Community Standard. 
 

 

Inhibitor-Free DNA from Any Sample:

 Isolate ultra-pure DNA ready for any downstream application. 

 

 

Certified Low Bioburden: 

Boost your detection limit for low abundance microbes. 

 

 

Simple Workflow:

 Simply bead-beat sample, purify via spin-column, and filter to remove PCR inhibitors.  

No precipitation or lengthy incubations! 

 
 

      

Contents 

 

 

 

 

 
Product Contents & Specifications ................................. 1 

Product Description ..................................................... 2-3 

Protocol ........................................................................ 4-5 

Appendices 

A. Sample Collection ............................................... 6

 

B. Application Notes ........................................... 7-10

 

C. Standardize Sample Preparation with

 

ZymoBIOMICS

 Microbial Standards ....... 11-12 

D. Troubleshooting ........................................... 12-13 

Ordering Information ..................................................... 14 

 

INSTRUCTION MANUAL 

Summary of Contents for ZymoBIOMICS D4300

Page 1: ...tream application Certified Low Bioburden Boost your detection limit for low abundance microbes Simple Workflow Simply bead beat sample purify via spin column and filter to remove PCR inhibitors No pr...

Page 2: ...beating device is recommended by using the ZymoBIOMICS Microbial Community Standard see Appendix C DNA Purity High quality inhibitor free DNA is eluted with ZymoBIOMICS DNase RNase Free Water and is s...

Page 3: ...n downstream metagenomic analyses that is not reflective of actual abundance 2 See endospore lysis efficiency data in Appendix B 3 For more information on the ZymoBIOMICS Microbial Community Standard...

Page 4: ...liably isolates DNA from even the toughest to lyse Gram positive organisms enabling unbiased analyses of microbial community compositions There is a significant increase in yield and Gram positive bac...

Page 5: ...ICS DNA Binding Buffer to the filtrate in the Collection Tube from Step 4 Mix well Add 800 l of ZymoBIOMICS DNA Binding Buffer and 400 l of 95 ethanol to the filtrate in the Collection Tube from Step...

Page 6: ...DNA5 6 12 Place a Zymo Spin III HRC Filter in a new Collection Tube and add 600 l ZymoBIOMICS HRC Prep Solution Centrifuge at 8 000 x g for 3 minutes 13 Transfer the eluted DNA Step 11 to a prepared Z...

Page 7: ...e of a potentially biohazardous swab material Cat No R1106 R1107 Figure 4 A Nucleic acids in stool are effectively stabilized in DNA RNA Shield at room temperature Graph shows spike in DNA and RNA con...

Page 8: ...MICS Lysis Tubes 0 1 0 5 mm and incubate for 30 minutes at 55 C 3 Continue to Step 2 page 4 and proceed with the protocol as written Plant Tissue Leaves and other plant material Plant tissues such as...

Page 9: ...Tube 0 1 0 5 mm to be used in Step 2 below c Re suspend pellet in a buffer suitable for enzymatic treatment ex PBS or other isotonic solution 2 Transfer the digestion mixture to a ZR BashingBead Lysis...

Page 10: ...isrupters FastPrep 24 or as long as 20 minutes when using lower speeds e g Disruptor Genie 3 Transfer the entire lysate to the ZR BashingBead Lysis Tube 0 1 0 5 mm proceed to Step 2 page 4 and continu...

Page 11: ...ep 2 2 Centrifuge at 3 000 x g for 15 minutes 3 Without disturbing the pellet slowly decant or pipette out the supernatant leaving behind 100 400 l of pellet 4 Add ZymoBIOMICS Lysis Solution to a fina...

Page 12: ...ty DNA Standard Cat No D6305 is a mixture of genomic DNA extracted from pure cultures of eight bacterial and two fungal strains Genomic DNA from each culture was quantified before mixing The ZymoBIOMI...

Page 13: ...uencing Figure 9 A Library preparation for shotgun metagenomic sequencing was performed in two different ways one by supplier I and one by an in house method Shotgun sequencing was performed on Illumi...

Page 14: ...ting Ensure that none of the debris is transferred to the Zymo Spin III F Filter in the next step Low DNA Yield Input If the lysate does not pass through the column or is extremely viscous use less in...

Page 15: ...mark of Qbiogene Inc Illumnia MiSeq Illumnia NexTera XT are trademarks or registered trademarks of Illumina Inc AcroMetrix is a trademark of Thermo Fisher Scientific Inc ZymoBIOMICS DNA Miniprep Kit i...

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