ZEISS
3 Product and Functional Description | 3.2 Main Components
NOTICE
Risk of collision
Use the chamber CCD camera to monitor the position of the specimen holder during stage
movements. Pay particular attention to the distance between the objective lens and the top of
the specimen. This applies to vertical movements, but also to horizontal movements, because a
thick specimen may collide with the objective lens from the side.
Depending on the selected detector, the chamber CCD camera is capable of acquiring black-and-
white images or colored images.
The performance of the diode detectors is negatively effected by the illumination that is required
for the camera. If a diode detector is selected, then by default the chamber CCD camera is dis-
abled:
Fig. 39: Chamber
camera disabled as indicated by a pause sign (e.g. if a diode detector is
selected).
3.2.6 Specimen Stage | Sigma 300
WARNING
Suffocation hazard due to lack of oxygen
Gaseous dry nitrogen is used to vent the specimen chamber during specimen exchange. Inhal-
ing nitrogen may cause unconsciousness.
4
During specimen exchange, keep the chamber door open as short as possible.
4
Do not inhale the air from within the specimen chamber.
4
Ensure that the area around the microscope is sufficiently vented.
4
If you begin to experience symptoms of asphyxia (for example: rapid breathing, loss of
mental alertness and/or muscular coordination, depression of sensations, emotional insta-
bility, fatigue) leave the room immediately and inform the facility’s safety officer.
CAUTION
Moving the specimen stage
Fingers can be trapped by the moving specimen stage.
4
Always close the chamber door before moving the specimen stage.
4
To remove parts fallen into or near to the stage use a tool (e.g. tweezers) instead of your
fingers.
Instruction Manual ZEISS SIGMA series | en-US | Rev. 7 | 352102-9344-006
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