Zeiss LSM 880, Operating Manual, Page: 69 / 690

CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
10/2014 V_01 000000-2071-464 63
(2) Microscope settings on Axio Imager for reflected-light observation (Epi-fluorescence)
•
Turn on the HBO 100 W or X-Cite power supply with switch (Fig. 61/ 8 )
•
Click on the RL reflected light button. The shutter opens.
To avoid excessive bleaching of biological samples, expose the specimen to the minimum
possible irradiation, i.e. keep the irradiation time as short as possible. For this, use the slider in
the Ocular tool to attenuate the illumination of the HBO / X-Cite.
•
By clicking on the reflector turret button, select the reflector module (filter sets) to suit the type of
fluorescence excitation. Proceed as follows:
•
Click on the reflector turret button.
•
Click on the desired reflector module.
−
The reflector turret moves the selected reflector module into the beam path.
The FITC filter set consists of an excitation filter for the 450 - 490 nm spectral range, an FT color
splitter for 510 nm and an LP long pass filter, which passes emission light wavelengths greater
than 510 nm (FSET 09 = FITC, FSET 15 = Rhodamine, FSET 01 = DAPI).
Other filter sets:
DAPI:
BP 365 FSET01
FT 395
LP 397
Rhodamine: BP 546 FSET15
FT 580
LP 590
The filter sets described in this section are examples; other sets are available on request.
The aperture setting on the condenser of the Axio Imager.Z2 is performed in fixed steps.
5.1.3 Controls for Axio Examiner
For setting the Axio Examiner, proceed in the same way as with Axio Imager.Z2 and Axio Observer.Z1.
Since the objective turret of the Axio Examiner is not motorized the setting of the desired objective has to
be done manually.
The objective in use must be indicated in the
Acquisition Mode tool.