ANNEX
LSM
510
DuoScan
Carl Zeiss
NLO Non Linear Optics Laser for LSM 510 NLO …
LSM 510 META
DuoScan
8-28
B 45-0021 e
03/06
8.8.3
Pinhole and Collimator Settings
•
The pinhole can be fully opened for maximum detection efficiency due to the focal excitation
capabilities of the NLO effect (see
Scan Control
window,
Channel Settings
).
•
In the
Pinhole & Collimator Control
window, the
NIR
collimator can be used to align the overlap of
the excitation planes within the object for VIS as well as NIR excitation light wavelength in the
Collimator
panel (see
Maintain
menu,
Pinhole
button).
8.9
Non Descanned Detection (NDD)
The application of Non-Descanned Detection with
the LSM 510 is only useful in combination with the
optional NLO laser.
The Non-Descanned Detection modules can be
used on the reflected or transmitted-light beam
path or simultaneously on both beam paths. This
means that the maximum of four NDD channels
can be configured. If two NDD channels have been
assigned to the transmitted-light beam path, no
transmission PMT can be implemented.
In Non-Descanned Detection, the radiation emitted
by the specimen is conducted directly on the
relevant detector without passing the scanner
mirror again.
Non-Descanned Detection is set and configured in
the
Configuration Contro
l window by activat-
ing / deactivating the buttons
Descanned Detec-
tion
and
Non-Descanned Detection
while the
NDD module is being connected.
Fig. 8-14
Configuration Control window
