ANNEX
LSM
510
DuoScan
Carl Zeiss
Beam Path Configuration Guide for ...
LSM 510 META
DuoScan
8-6
B 45-0021 e
03/06
Line and Frame Mode of Multitracking
•
Settings can be used for
Line
or
Frame
wise Multitracking.
•
In Line mode the lines are scanned in turns for all tracks with the corresponding laser lines turned on
exclusively. Preferred for living samples with moving objects. Acquisition time can be reduced using
bidirectional scan mode.
•
In Frame mode whole frames are scanned in turns for all tracks with the corresponding laser lines
turned on exclusively. This mode can be advantageous for dyes that tend to bleach and need time to
recover.
•
There are parameters that can be changed quickly in a line wise: Amplifier Gain, Amplifier Offset,
Laser Line Attenuation,
•
Any other changes of track settings of the selected tracks, e. g. different filters, dichroics or Detector
Gain settings, need a bit more time to be changed, and require Frame mode.
•
There is a fast Frame mode, that requires identical settings of these parameters.
•
In our example it is now possible to use the BP 505-550 also in track two. There is no function of this
BP in track two, but it guarantees equal settings in both tracks/channels, which now allows line wise
Multitracking.
8.1.4
Beam path Configuration for Multi Photon Excitation
•
The beam path configuration for multi photon excitation follows the same rules as described for a
single and multi track configuration (see above).
•
Use a KP 650 or KP 700 as main dichroic beam splitter to deflect the IR excitation light (700-
1050 nm) onto the specimen.
•
On detection side, set always a BG 39 in the beam path or use a IR suitable band pass filters
(BP XXX-YYY IR) to prevent a bleed through of IR excitation light to the detector.
