LSM 510
Routine Mode
LSM 510 META
Application of Examination Methods in …
Carl Zeiss
03/06
B 45-0021 e
6-5
6.1.3.1
Acquisition of a Frame
(1)
Selection of the examination method
(a)
Standard Examination Methods
•
Click on the button of the required standard
examination method, e.g.
FITC / Rhodamine
.
In case the current system has a META detector in
channel 1 one has to define a method first for the
routine mode.
−
The
Routine Mode - Microscope Setup
window will appear.
(b)
User Defined Examination Methods
•
Click on the name of the examination method
in the selection box of the
User-Defined
Examination Methods
panel, e.g.:
Convallaria
.
•
Then click on the
Next
button.
−
The
Routine Mode - Microscope Setup
window will appear.
(2)
Routine Mode - Microscope Setup
window
•
Prepare your specimen for examination in the
same way as in the Expert mode, (see
chapter 4). As in the Expert mode, different
windows are available for the Axio Imager.Z1,
Axioskop 2 FS
MOT
and the Axiovert 200 M.
•
Set the microscope parameters for transmitted
light or reflected light by clicking on the appro-
priate graph (see chapter 4).
•
Clicking on the
Stage
button enables you to
open the
Stage and Focus Control
window
and to perform motorized focusing and
specimen positioning.
The
Stage and Focus Control
window is identical
to that of the Expert Mode and is handled in the
same way (see chapter 4).
The
Stage
button is also available in the
Image
Setup, Image Stack Setup
and
Time Setup
windows.
Fig. 6-5
Select Examination Method
window
Fig. 6-6
Microscope Setup window
