LSM 510
OPERATION IN EXPERT MODE
LSM 510 META
3D View Menu
Carl Zeiss
03/06
B 45-0021 e
4-171
As the resolution of an optical system is
significantly lower in the axial direction than in the
lateral (X/Y-)direction, the greatest improvement in
resolution can be achieved in the Z-direction.
The Z Stack must meet the following requirements:
−
At least two-fold oversampling in xyz (z: half
of optimal interval button)
−
High signal-to-noise ratio
−
Detector gain < 500 V
Calculation is either made for one channel of the
opened image which must first be selected
accordingly, or for all channels of a stack.
Calculation is started via
Apply
and can be
stopped using the
ESC
key, if required.
4.7.4.1
Open / Close the 3D Deconvolution
Window
•
Click on the
DCV
button in the
Process
subordinate toolbar of the
Main
menu.
−
This opens the
3D Deconvolution
window.
•
Click on the
Close
button to quit the window.
Fig. 4-165
Image of a fluorescent bead with a
diameter of 1µm before deconvolution
(A,B) and after deconvolution (C,D)
Fig. 4-166
3D Deconvolution window
