LSM 510
OPERATION IN EXPERT MODE
LSM 510 META
Acquire Menu
Carl Zeiss
03/06
B 45-0021 e
4-121
•
Enter the number of iterations of the bleaching
procedure in the
Iterations
input box.
•
Click on the
Define Region
button.
−
The
Bleach Regions
window appears.
The definition of bleach regions corresponds to the
Edit ROI
function and is performed in the same
way (see
Edit ROI
, page 4-100).
ROIs already defined with
Edit ROI
are also
available in the
Bleach Regions
window. They can
be activated directly, modified - if required - and
stored under a new name.
ROIs newly defined in
the Bleach
Regions
window will then also be
available in the
Edit ROI
window.
•
Define the required bleach regions in the scan
image or use an existing ROI.
4.5.7.4
Excitation of Bleach Track Panel
In the
Excitation of Bleach Track
panel you can
select the lasers and laser intensities for bleaching.
If the system is equipped with LSM
DuoScan
and the check box
Simultaneous Grab and Bleach
is
ticked, only those lasers are displayed
which are available through the LSM
DuoScan
.
The setting of the lasers for the bleaching
procedure corresponds to that for the scanning
procedure and must be performed accordingly (see
Laser Control
,
Configuration Control
and
Scan
Control
).
•
Select the required laser wavelength and its
intensity under
Excitation
.
•
If required, switch the relevant laser to
On
(
Laser
button).
•
If more than one ROI has been defined under Bleach Parameters, a different laser intensity and laser
line can be defined for each region. Toggle between the Buttons indicating the number of the ROI and
check the laser line and intensity for each ROI.
Fig. 4-100
Bleach Regions window
Fig. 4-101
Excitation of Bleach Track panel
