OPERATION IN EXPERT MODE
LSM 510
DuoScan
Carl Zeiss
Acquire Menu
LSM 510 META
DuoScan
4-60
B 45-0021 e
03/06
(5)
Beam path - Laser attenuation
•
On the
Beam Path and Channel Assignment
panel, click on the
Excitation
button.
−
This opens a dialog box of all available lasers
with their wavelengths and their usable
Acousto-Optical Tunable Filters (AOTF)
attenuation.
•
To select the desired laser line, activate the
check box for
Line Active
.
•
Use the
Transmission [%]
slider to set the
utilizable laser intensity (recommendation: start
at 10 %).
−
The transmittance of the Acousto-Optical
Tunable Filter (AOTF) changes accordingly.
•
This allows you to adapt the laser intensity very sensitively to the job. Activate the check box for
Line
Active
.
−
This activates the selected laser power for use. This is indicated by the
Laser Power
display lamps
(status display green / gray).
By clicking on the
Excitation
button you can check at any time which lasers are available for
active operation.
If you deactivate
Line Active
, the laser wavelengths are deselected by means of the Acousto-
Optical Tunable Filters (AOTF), i.e. these lasers change into standby status.
If you interrupt your work with the LSM for a break, it is recommended not to switch the
Enterprise and argon lasers off by hardware action, but to put them into standby status as
described.
Excitation filters, emission filters, HFT main dichroic beam splitters and NFT secondary dichroic
beam splitters can be switched online, channels (PMT photomultipliers) only off-line.
Fig. 4-43
Configuration Control window
