OPERATION IN EXPERT MODE
LSM 510
DuoScan
Carl Zeiss
Acquire Menu
LSM 510 META
DuoScan
4-46
B 45-0021 e
03/06
Microscope settings on Axio Imager for reflected-light observation (Epi-fluorescence)
•
Turn on the HBO 100 W power supply with switch (4-29/
1
).
•
Click on the RL reflected light button
.
The shutter opens.
To avoid excessive bleaching of biological samples, expose the specimen to the minimum
possible irradiation, i.e. keep the irradiation time as short as possible. For this, close the field
stop to the necessary field of illumination and use additional filters if available.
•
By clicking on the reflector turret button, select the reflector module (filter sets) to suit the type of
fluorescence excitation. Proceed as follows:
•
Click on the reflector turret
button.
•
Click on the desired reflector module.
−
The reflector turret moves the selected reflector module into the beam path.
The FITC filter set consists of an excitation filter for the 450 - 490 nm spectral range, an FT color
splitter for 510 nm and an LP long pass filter, which passes emission light wavelengths greater
than 510 nm (FSET 09 = FITC, FSET 15 = Rhodamine, FSET 01 = DAPI).
Other filter sets:
DAPI:
BP 365 FSET01
FT
395
LP
397
Rhodamin: BP
546
FSET15
FT
580
LP
590
The filter sets described in this section are included in the standard configuration, but other sets
are available on request.
If the X-Cite 120 fiber coupled lamp for
reflected-light illumination is used a
further dialog is opened when clicking
the Reflected light button. The lamp
internal shutter and the attenuation
function can be controlled via the LSM
Software. The lamp has to be switched
on first using the main switch on the
lamp housing.
Fig. 4-30
X-Cite 120 control panel
