Zeiss Lightsheet Z.1, Operating Manual

Page: 43 / 427

CHAPTER 1 - HARDWARE
Lightsheet Z.1 User Interfaces Carl Zeiss
02/2013 000000-1790-528 39
Heating component Temperature range Incubation modules
Heating Block Heating from ambient
temperature to 42 °C
(1) TempModule S1
(2) Heating Block LSFM
Peltier Block Heating and cooling in the
range from 10 °C to 42 °C
(1) TempModule S1
(2) TempModule CZ-LSFM (including Peltier
Block and Temperature sensor)
Tabelle 1 Overview Heating components
3.7.2 CO
2
-Module Lightsheet Z.1
The CO
2
-Module Lightsheet Z.1 controls and monitors adjustable CO
2
concentration (from 0 % to 10 %)
in the sample chamber and requires CO
2
supply, sterile filter and Humidifier S1 to provide CO
2
-enriched
air.
−
At least 3h (or more) may be needed until a stable pH value is reached due to the small surface
area in relation to sample chamber volume.
−
For assembly of hardware components of the CO
2
Module and its connections to the system, as
well as the calibration procedure needed follow the instructions from the PeCon manual.
−
After CO
2
calibration, the Lightsheet Z.1 system must be completely turned off before work with
the system can continue.
−
Position the Luer-Lock connector for CO
2
incubation at the top right corner of the sample chamber.
Place a
cover
on top of the sample chamber body (Fig. 24, chapter 3.3.4) to maintain the CO
2
gas
concentrations.
−
The sterility of the CO
2
-enriched air is given by the sterile filter. Take control of the sterility in your
sample chamber by following the cleaning instruction of all parts of the CO
2
-module (see PeCon
manual) as well as the components of the sample chamber (see CHAPTER 1 HARDWARE).
Please clamp all incoming or outgoing tubing (Fig. 32/ 2 , Fig. 32/ 5 , Fig. 32/ 9 , Fig. 32/ 10 ) into the
provided clamping grooves. This will avoid possible disconnection problems after closing the front
system cavity door.
Note that for the sake of completeness Fig. 26 shows the connection diagram for the usage of a
perfusion pump (Fig. 32/
5 , Fig. 32/ 9 ). Alternatively a syringe based-perfusion can be used (position
Fig. 32/ 5 ). Consequently, Fig. 32/ 9 has to be replaced by a bling plug.