Lightsheet Z.1
Carl Zeiss
02/2013
000000-1790-528
25
6.
Repeat step 1 - 6 for each of the three windows.
−
Assemble the sample chamber window for the
detection optic
.
1.
Turn the sample chamber body (Fig. 19/
1
) to the largest opening window for the detection
optics.
2.
Using a small spatula or the opposite end of a forceps position the blue O-ring tightly into
the groove to ensure no leaking (Fig. 19/
2
).
3.
Assemble the chamber window for the detection optic according to the following steps.
Depending on the detection optics used, insert the correct detection optic adapter:
4.
For the detection optic 5x/0.16:
a)
Place the large silver detection optic adapter 5x (Fig. 19/
3
) onto the table with the
grooves facing you.
b)
Remove one clean circular 18 mm glass cover slip; place cover slip (Fig. 19/
4
) into
the large silver detection optic adapter 5x (Fig. 19/
3
) making sure it is positioned
into the smallest groove – adjust the coverslip position with the forceps if necessary.
c)
Now place the O-ring (18 mm O-ring, black) into the corresponding groove on top
of the cover slip (Fig. 19/
5
).
d)
Take the silver retaining ring (Fig. 19/
6
) and place it inside the detection optics
adapter 5x with the flat side with two notches facing you. Using the circular pin-side
of the sample chamber window tool (Fig. 19/
9
), turn clockwise until finger-tight.
e)
Placing the assembled detection optic adapter 5x into the sample chamber body
window. Using the squared pins of the sample chamber window tool, turn clock-
wise until finger-tight.
5.
For the detection optic 20x/1.0, 40x/1.0, 63x/1.0:
a)
Use the corresponding detection optic adapter (see the detection optic adapter
catalogue number and magnification engraving) (Fig. 19/
7
).
b)
Insert the detection optic adapter into the sample chamber body window for
detection with the flat side facing you.
c)
Using the sample chamber window tool (squared pin-side) (Fig. 19/
9
) turn adapter
clock-wise until finger-tight.
d)
Take a 15 mm black O-ring (Fig. 19/
8
) and position the O-ring into the groove inside
the middle of the detection optic adapter using the blunt end of a forceps if
necessary.
Summary of Contents for Lightsheet Z.1
Page 1: ...Lightsheet Z 1 Operating Manual February 2013 ZEN 2012 black edition ...
Page 4: ......
Page 170: ......
Page 427: ...Lightsheet Z 1 Overview ...
