Zeiss Lightsheet Z.1, Operating Manual

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CHAPTER 1 - HARDWARE
Lightsheet Z.1 User Interfaces Carl Zeiss
02/2013 000000-1790-528 15
−
Now start the ZEN (black edition) software
and perform the following tasks found in the
Maintain
tab:
•
Detector recognition
•
Automatic Detector Alignment; and
if necessary
•
Manual Detector Alignment.
−
It might be necessary to adjust the focus
of the calibration grating (Fig. 8) for
the
Automatic or Manual Detector
Alignment task . Start one of these tasks to
get a live image of the grating for evaluating
and adjusting the focus if applicable (see
section
3.2.2 Adjustment – Manual
Detector Alignment ).
3.2 Adjustment – Detector Recognition
When the detection modules are exchanged or attached to the Lightsheet Z.1 system for the first time,
the system must identify which detection module is present for which channel. Pressing the button
Detector recognition will start an identification routine to do this job. A progress bar is visible during
the process. When the
Cancel
button is pressed, the operation is aborted without saving any result. This
routine must be conducted before the Automatic or Manual Detector Alignment. At the end of this
process you be informed if the detector recognition had to perform any changes. If so, please restart the
ZEN software before continuing.
3.2.1 Adjustment – Automatic Detector Alignment
This adjustment is necessary for one or two detection modules, when:
•
detection modules have been exchanged
•
reflector turrets have been removed and inserted (restart ZEN before the alignment task)
•
recognition of a pixel shift between channels
•
the zoom is not centered.
No sample should be present. The necessary correction plate and grating is present for each channel
within the system and needs no additional activation.
The Automatic Detector Alignment will align the image center with the detection module center (zoom
center). For two detection modules, it will additionally align both to have overlapping pixels.
After detection module exchange, first press the
Detector recognition
button in the
Adjustment
tool window.
Fig. 7 ZEN Start-up window