Zeiss Lightsheet 7, Installation Requirements

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2 Requirements | 2.6 Gas Supplies ZEISS
2.5.2 Biosafety Level of the Laboratory
The customer should inform ZEISS of the biosafety level of the installation site. Access to the sys-
tem, safety training, correct safety dress code and use of tools and test equipment needs to be
clarified for specified laboratories (World Health Organisation Laboratory Biosafety Manual 3).
Biosafety Level Description
1 Suitable for work with well-characterized agents which do not cause
disease in healthy humans. In general, these agents should pose mini-
mal potential hazard to laboratory personnel and the environment.
2 Laboratories maintain the same standard microbial practices as BSL-1
labs, but also includes enhanced measures due to the potential risk of
the aforementioned microbes. Personnel working in BSL-2 labs are ex-
pected to take even greater care to prevent injuries such as cuts and
other breaches of the skin, as well as ingestion and mucous mem-
brane exposures.
3 Laboratory typically includes work on microbes that are either indige-
nous or exotic, and can cause serious or potentially lethal disease
through inhalation. Examples of microbes worked within a BSL-3 in-
clude yellow fever, West Nile virus, and the bacteria that causes tuber-
culosis.
4 Labs are rare. However, some do exist in a small number of places in
the US and around the world. As the highest level of biological safety,
a BSL-4 lab consists of work with highly dangerous and exotic mi-
crobes. Infections caused by these types of microbes are frequently
fatal, since there are no available treatments or vaccines. Two exam-
ples of such microbes include Ebola and Marburg viruses.
Tab. 3: Biosafety Level
2.6 Gas Supplies
Make sure the gas bottles are stored safely in or outside of the building according to local safety
and fire safety regulations.
The gas regulators should be supplied by the User.
The connection must be equipped with an appropriate pressure reducer and a main shut-off valve
that is secured against accidental re-activation, e.g. a ball valve AKT 10TN88 with optional lock or
equivalent. The user is responsible for the installation of a main shut-off valve at the site of instal-
lation, he/she should also provide instructions how to operate the main shut-off valves properly.
Main shut-off valves have to be easily accessible. They must close off the connections to the cor-
responding media when needed. The main shut-off valves have to be lockable in their off position
in order to prevent accidental re-activation.
Shut-off valves should be mounted near the Microscope System in such a way that the person ac-
tuating or inspecting the shut-off valve is not exposed to risks.
14 Installation Requirements ZEISS Lightsheet 7 | en-US | Rev. 1 | 349500-8186-000