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ZEISS ELYRA

Sample Preparation for Superresolution Microscopy –
a Quick Guide

White Paper

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Summary of Contents for ELYRA

Page 1: ...ZEISS ELYRA Sample Preparation for Superresolution Microscopy a Quick Guide White Paper...

Page 2: ...ision Cover Glasses 18x18 mm ZEISS order Number 474030 9010 000 Marienfeld Superior www marienfeld superior com 18x18 mm Cat No 0107032 22x22 mm Cat No 0107052 round with 18 mm diameter Cat No 0117580...

Page 3: ...or fluorescent proteins are suitable to be used for SR SIM Make sure to have a highly specific labeling with low background to obtain a good signal to noise ratio For multicolor samples the fluoropho...

Page 4: ...h RI of 1 44 ProLong Gold Life Technologies Be aware this embedding medium needs 2 day to harden in order to reach a constant RI In addition your sample may shrink during this process Refractive index...

Page 5: ...0 17 14 C All these formats will fit into the adjustable piezo stage insert Please be aware in order to obtain ideal TIRF illumination you need a sufficient mismatch in refractive indices In addition...

Page 6: ...3 Examples of fluorescent proteins that can be used for PALM imaging Most of these proteins are available through e g Addgene Cambridge MA 02139 USA Recommended FP Pairs for dual color PALM Pair 1 mE...

Page 7: ...of number of photons per on switching event and the on off duty cycle on STORM image quality for an example structure a ring like object p t and x Images of CCPs clathrin coated pits using Alexa Fluor...

Page 8: ...Glucose Oxidase e g 24 mg ml GluOx from Aspergillus niger Sigma G0543 50KU 5 l Catalase e g 12 6 mg ml Catalase from Bovine liver Sigma C3155 50 MG Add H2O to final volume of 1ml Very important adjust...

Page 9: ...to dH2O solution vortex FM solution coat cover slips with Poly L lysine PLL for adhesion of FM add FM Mix solution onto PLL coated cover slips check density 1 3 FM in field of view prepare sample on...

Page 10: ...ent protein J of Microcopy 2011 3 Shroff et al Current Protocols in Biology 2010 Sample Preparation and Fluorophore Properties for dSTORM 1 G T Dempsey et al Evaluation of fluorophores for optimal per...

Page 11: ...scopy GmbH 07745 Jena Germany BioSciences microscopy zeiss com www zeiss com elyra EN_41_001_065 CZ 12 2013 Design scope of delivery and technical progress subject to change without notice Carl Zeiss...

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