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Zeiss Axiolab 5 Operating Manual Download Page 132

 

APPENDIX 

ZEISS 

Industrial property rights 

Axiolab 5 

 

 
 

132 

430037-7444-001 

05/2019 

Setting up ...........................................................................................................................................51 

Slot ....................................................................................................................... 22, 24, 26, 28, 30, 32 
Snap button .......................................................................................................... 22, 24, 26, 28, 30, 32 

Specimen guide ............................................................................................................................. 39, 59 

Specimen holder ........................................................................................................................... 38, 39 
Specimen holding plate .......................................................................................................................39 

Stage ..................................................................................................................................................21 
Stage carrier ................................................................................... 22, 24, 26, 28, 30, 32, 57, 59, 60, 62 

Stage clip ............................................................................................................................................59 
Stand ..................................................................................................................................................21 

Stand models ......................................................................................................................................21 

Stands keys .........................................................................................................................................34 
Start-up ..............................................................................................................................................51 

Switching on/off ..................................................................................................................................76 

T

 

Technical data .....................................................................................................................................17 

Tool ....................................................................................................................................................52 
Tool kit storage ..................................................................................................... 22, 24, 26, 28, 30, 32 

Transmitted light .................................................................... 22, 24, 26, 28, 30, 32, 81, 87, 89, 98, 109 
Transmitted light (TL) button.................................................................................................... 24, 30, 32 

Transmitted light illumination .................................................................................................. 24, 26, 28 
Transmitted light illuminator .................................................................................................... 22, 30, 32 

Transmitted light phase contrast ..........................................................................................................87 

Troubleshooting ................................................................................................................................ 121 
Tube ......................................................................................................... 21, 22, 24, 26, 28, 30, 32, 54 

U

 

Unpacking ..........................................................................................................................................51 

V

 

Vertical adjustment of condenser ........................................................................... 22, 24, 26, 28, 30, 32 
Viewing height .............................................................................................................................. 19, 79 

W

 

Warning label .....................................................................................................................................12 
Warranty .............................................................................................................................................14 

Weight ...............................................................................................................................................17 
 
 

6.3

 

Industrial property rights  

Instruments, instrument components and  methods described in this manual are protected by the 

following patents: 

−

 

see label on microscope stand 

 
 

6.4

 

System overview 

See the following pages. 

 

 

Summary of Contents for Axiolab 5

Page 1: ...Operating Manual Axiolab 5 Upright Microscope for Routine and Entry level Research Microscope for Routine and Entry Level Research...

Page 2: ...copyright are liable to pay compensation for damages All rights reserved including rights created by patent grant or registration of a utility model All names of companies and products mentioned in t...

Page 3: ...ocular tubes photo tubes 35 2 5 2 Microscope stages 38 2 5 3 Filter mount on luminous field diaphragm operating ring for filter 32x4 mm 40 2 5 4 Condensers 40 2 5 5 Filter slider for reflected light s...

Page 4: ...binocular tube 79 4 1 2 Setting the viewing height 79 4 1 3 Adjusting for ametropia user s visual impairment when using eyepiece reticles 80 4 2 Illumination and contrast methods in transmitted light...

Page 5: ...lar photo tube 30 23 with fixed graduation 50 50 35 Fig 2 9 Binocular photo tube 20 23 with toggle graduation 100 0 0 100 35 Fig 2 10 Binocular photo tube 30 23 with toggle graduation 100 0 0 100 36 F...

Page 6: ...22 Swinging the loops out 68 Fig 3 23 Changing the LED illuminator 68 Fig 3 24 Changing the LED illuminator in the adapter 69 Fig 3 25 Removing the module holder 70 Fig 3 26 Changing the LED modules 7...

Page 7: ...on on the conoscopy stand 102 Fig 4 14 Determining the polarization direction n using a synthetic fiber as an example 103 Fig 4 15 Schematic diagram of the color charts according to Michel L vy 105 Fi...

Page 8: ...anual contains information and safety warnings with which the operator must comply The following warning instructional and informational symbols are used in this manual CAUTION Warns the operator of a...

Page 9: ...ts which adapt to line voltages ranging from 100 V to 240 V and frequencies ranging from 50 to 60 Hz No voltage adjustment is required on the microscopes themselves CAUTION Always disconnect the micro...

Page 10: ...tely Do not allow it to contaminate surface water or enter drains or the sewage system CAUTION Do not bring any flammable or easily combustible materials into the light beam CAUTION This device belong...

Page 11: ...ay result in heat accumulation which could damage the instrument and even start a fire Ensure that the ventilation slits are always kept open uncovered and clear Do not insert or drop anything into th...

Page 12: ...olab 5 12 430037 7444 001 05 2019 1 2 Warning labels on the microscopes NOTE Warning label Hot surface Affixed to all stands with transmitted light halogen illumination Fig 1 1 Warning labels on the A...

Page 13: ...INTRODUCTION Axiolab 5 Warning labels on the microscopes ZEISS 05 2019 430037 7444 001 13 Fig 1 2 Warning labels on the Axiolab 5 stand for transmitted light...

Page 14: ...g parts and to improper handling are not covered by our warranty The manufacturer is not liable for damage to the instrument due to incorrect operation negligence or any other manipulation of the inst...

Page 15: ...he instrument configuration the following microscopy and contrasting techniques can be used Transmitted light Reflected light Brightfield BF Brightfield BF Darkfield DF Darkfield DR Phase contrast Ph...

Page 16: ...le ergo tubes Latex free skin friendly surfaces on the binocular section of the tubes control elements and stand stage drives whose height and friction can be adjusted optional use of fine focusing kn...

Page 17: ...Ambient conditions Shipping in packaging Permissible ambient temperature 40 to 70 C Permissible humidity without condensation max 75 at 35 C Storage Permissible ambient temperature 10 to 40 C Permiss...

Page 18: ...d light fluorescence with replaceable LED modules Wavelengths optional 385 470 565 625 nm LED classification LED Risk Group 2 to IEC 62471 Axiolab 5 Stand with manual stage focusing Coarse focusing ap...

Page 19: ...0 100 20 None 442 481 425520 9050 000 Binocular ergo tube 15 23 50 50 telescopic height upright image 15 Height telescopic 410 509 425520 9090 000 Binocular tube 20 23 20 442 481 425520 9100 000 Binoc...

Page 20: ...Axiolab 5 20 430037 7444 001 05 2019 2 3 Interface diagram The following figure shows a diagram of the interfaces of the microscope stand The Axiolab 5 Mat TL RL stand is used here as an example Fig 2...

Page 21: ...Eight stand models are available in the delivery program 1 Axiolab 5 stand Bio TL XY stage with right handle 430037 9011 000 2 Axiolab 5 stand Bio TL XY stage with left handle 430037 9060 000 3 Axiola...

Page 22: ...tand 8 Intensity LM knob for light intensity and Light Manager function LM 9 Indicator light 10 Permanent ECO mode switch 11 Focusing drive fine adjustment right side finger wheel 12 Focusing drive co...

Page 23: ...DESCRIPTION OF THE INSTRUMENT Axiolab 5 Control and functional elements on the microscope ZEISS 05 2019 430037 7444 001 23 Fig 2 2 Axiolab 5 stand Bio TL...

Page 24: ...lder 7 Transmitted light illumination in stand base 8 Basic stand 9 Intensity LM knob for light intensity and Light Manager function LM 10 Reflected light RL button and indicator light for reflected l...

Page 25: ...DESCRIPTION OF THE INSTRUMENT Axiolab 5 Control and functional elements on the microscope ZEISS 05 2019 430037 7444 001 25 Fig 2 3 Axiolab 5 stand Bio TL FL...

Page 26: ...light illumination 7 Basic stand 8 Intensity LM knob for light intensity and Light Manager function LM 9 Indicator light 10 Permanent ECO mode switch 11 Focusing drive fine adjustment right side 12 Fo...

Page 27: ...DESCRIPTION OF THE INSTRUMENT Axiolab 5 Control and functional elements on the microscope ZEISS 05 2019 430037 7444 001 27 Fig 2 4 Axiolab 5 stand Pol TL...

Page 28: ...and lens in out 10 Rotary knob A Swiveling analyzer in out 11 Intensity LM knob for light intensity and Light Manager function LM 12 Indicator light 13 Permanent ECO mode switch 14 Focusing drive fine...

Page 29: ...stand Pol TL Conoscopy ATTENTION The movements of rotary knobs A and BL Fig 2 5 9 and 10 and the respective setting wheels Fig 2 5 33 and 32 are coupled with each other This means that only one contro...

Page 30: ...illumination 9 Tool kit storage flap cable holder 10 Transmitted light illuminator in stand base 11 Basic stand 12 Intensity LM knob for light intensity and Light Manager function LM 13 Reflected lig...

Page 31: ...DESCRIPTION OF THE INSTRUMENT Axiolab 5 Control and functional elements on the microscope ZEISS 05 2019 430037 7444 001 31 Fig 2 6 Axiolab 5 stand Pol TL RL...

Page 32: ...n 9 Tool kit storage cable holder 10 Transmitted light illuminator in stand base 11 Basic stand 12 Intensity LM knob for light intensity and Light Manager function LM 13 Reflected light RL button with...

Page 33: ...DESCRIPTION OF THE INSTRUMENT Axiolab 5 Control and functional elements on the microscope ZEISS 05 2019 430037 7444 001 33 Fig 2 7 Axiolab 5 stand Mat TL RL...

Page 34: ...y LM knob see Fig 2 3 9 Turn Controls the light intensity of the active light source Long press Light Manager function Saves the light intensity after saving is done the LED switches off for 300 ms da...

Page 35: ...d graduation 50 50 Fifty percent of the light is directed to the eyepieces and fifty percent to the camera port Fig 2 8 Binocular photo tube 20 23 upright image with toggle graduation 100 0 0 100 The...

Page 36: ...camera symbol 100 light to camera Push pull rod Fig 2 10 1 pushed in eyepiece shutter closed Push pull rod Fig 2 10 1 pulled out eyepiece shutter opened Particularly for camera shots with extended exp...

Page 37: ...imum weight of 2 5 kg camera plus cable For a fee the ZEISS service personnel will equip your instrument with a higher maximum load if needed The ergo tubes allow a height adjustment range of 44 mm A...

Page 38: ...nal preferences of the user with regard to height and friction The required tools Fig 2 12 5 are located in the upper coaxial knurled knob Vernier scale for display of adjustment range in the X Fig 2...

Page 39: ...cimen guide Fig 2 15 4 and specimen holder Fig 2 15 6 accommodates standard 45x25 mm slides and 75x25 mm 3 x1 360 rotation with lock use knurled screw to lock Fig 2 15 5 Click stop Fig 2 15 7 every 45...

Page 40: ...9 1 25 BF DF Ph1 Ph2 Ph3 Condenser 0 9 1 25 BF Fig 2 17 1 with aperture diaphragm Fig 2 17 4 with modulator disk Fig 2 17 3 for brightfield microscopy BF darkfield microscopy DF phase contrast microsc...

Page 41: ...n be applied to the areas provided Fig 2 19 3 2 5 7 Low power system for objectives 2 5x 4x The low power system is for full display field illumination when using an objective with a weak magnificatio...

Page 42: ...larizer is moved too far down the polarizer may collide with the field diaphragm and damage it 2 5 9 Nosepiece with objectives Nosepiece 5x with M27 threaded insert for five objectives You can change...

Page 43: ...d and saved to the USB drive Functions of the microscope stand such as the Light Manager and encoding are automatically launched The camera is equipped with image enhancement functions such as true co...

Page 44: ...act as the control interface Functions such as the Light Manager encoding and image enhancement are automatically deployed Live images can be viewed on the monitor display and advanced features are av...

Page 45: ...ble electronic device that uses Wi Fi Functions such as the Light Manager encoding ECO mode and image enhancement are automatically launched When a monitor is connected live images can be viewed on th...

Page 46: ...PC or portable electronic device controlled via Ethernet or Wi Fi Functions such as the Light Manager encoding ECO mode and image enhancement are automatically launched When a monitor is connected liv...

Page 47: ...to the camera via a USB cable Functions such as the Light Manager encoding ECO mode and image enhancement are automatically launched When a monitor is connected live images can be viewed on the monito...

Page 48: ...r cable connected to an external power socket A workstation can be connected to the camera and the microscope stand via USB cables at the same time Functions such as the Light Manager encoding and ECO...

Page 49: ...via Commercial Micro D cable 2 port for camera control and image transfer USB 3 0 3 Gigabit Ethernet port RJ45 for communication and image transfer 4 HDMI port for image data transfer to a monitor TV...

Page 50: ...OSD functionality with Axiocam 202 mono 208 color The On Screen Display menu OSD menu is shown on whatever display the camera is connected to via an HDMI cable Press the OSD menu button Fig 2 24 3 to...

Page 51: ...described in the following section are illustrated using examples for one microscope stand type However they apply similarly to other microscope stand models Special features are described separately...

Page 52: ...3 2 2 at the back of the stand Pull the cover flap to open it push it to close The following tools are included in the delivery angled Allen wrench AF 3 two knurled Allen wrenches AF 1 5 for adjusting...

Page 53: ...sition Rotate the binocular tube into the desired observation position and re tighten the Allen screw with the Allen wrench Fig 3 3 Attaching the binocular tube For tubes mounted with an intermediate...

Page 54: ...hese diaphragms can be focused with the adjustable eye lens of the auxiliary microscope The auxiliary microscope Fig 3 4 3 or pinhole diaphragm Fig 3 4 4 can be used to observe conoscopic images Inser...

Page 55: ...imes the eyeglass protection rings are seated very tightly in the eyepiece groove so you may need a blunt specimen wooden stick to prod them off 3 1 4 Screwing in objectives Move mechanical stage with...

Page 56: ...module from below until it also securely engages with the lower spring clips of the reflector turret The position number of the P C module is shown on the right hand side of the reflector turret adja...

Page 57: ...8 4 using an Allen wrench AF 3 Fig 3 8 5 Remove stage Fig 3 8 3 upwards from the stage carrier 3 1 6 2 Installing the stage Place the stage Fig 3 8 3 on the stage carrier Fig 3 8 4 so that the thread...

Page 58: ...3 9 It should not be shifted more than one revolution 2 Y drive Push the coaxial knurled knob for the Y adjustment Fig 3 9 1 all the way to the top Insert the supplied adjusting pin Fig 3 9 5 into th...

Page 59: ...ovetail stage bottom on the spring loaded pin Fig 3 10 7 Attach the rotary stage with the clamp screw Fig 3 10 8 pointing to the front right Press the Pol rotary stage to the front against the spring...

Page 60: ...s procedure The K HLER illumination on the microscope must be adjusted before centering the stage see section 4 2 1 Turn the nosepiece to swing the non centering objective mount For centering the stag...

Page 61: ...reticle First turn the nosepiece to swing the non centering objective mount Center the rotary stage for the non centering objective mount as described under 3 1 7 5 Turn the nosepiece to move a center...

Page 62: ...w power system make sure that this does not come to rest on the luminous field diaphragm Insert the condenser Fig 3 13 8 or 9 between the condenser carrier Fig 3 13 3 and the stage carrier Fig 3 13 1...

Page 63: ...er Insert the darkfield condenser Fig 3 14 6 in the condenser holder Z Fig 3 14 5 and screw on the fastening ring Fig 3 14 7 Push the condenser holder Z Fig 3 14 5 against the spring box Fig 3 14 2 in...

Page 64: ...halogen lamp Fig 3 16 2 Press the loops Fig 3 16 1 on the securing clips of the lamp holder together and swing them out to the front When changing the lamp remove the old one Fig 3 16 2 Position the n...

Page 65: ...m the connector of the stand Pull the old LED illuminator with adapter Fig 3 17 3 out of the holding tube Fig 3 17 1 Fig 3 17 Removing the LED illuminator Loosen the three side screws Fig 3 18 4a 4b 4...

Page 66: ...lug the illuminator plug Fig 3 19 2 into the connector of the stand Lift the securing clips Fig 3 19 1 of the lamp holder until they are completely enclosed in the lamp holder In doing so slightly pre...

Page 67: ...lug Fig 3 21 3 from the halogen lamp Fig 3 21 2 Press the loops on the securing clips Fig 3 21 1 of the lamp holder together and swing them out to the front When changing the lamp remove the old one F...

Page 68: ...22 1 on the securing clips of the lamp holder together and swing them out to the front Remove the LED illuminator plug Fig 3 23 5 from the connector of the stand Fig 3 23 6 Pull the old LED illuminat...

Page 69: ...with the adapter front edge Fig 3 23 2 aligned to the right Plug the lamp plug Fig 3 23 5 into the connector of the stand Fig 3 23 6 Lift the securing clips Fig 3 22 1 of the lamp holder until they a...

Page 70: ...eed as follows Switch off the microscope remove the power cord on the microscope and allow it to cool down at least 15 min Remove the cover see also section 3 1 11 Unscrew the module holder Fig 3 25 1...

Page 71: ...the right side of the microscope see section 2 4 3 Fig 2 3 4 Plug the module plugs into the connectors Fig 3 26 1 2 of the stand Ensure that the plugs are connected to the right connectors Fig 3 26 Sc...

Page 72: ...3 27 4 of the tube Orient the camera to the stand and fix it in position by tightening the ring nut Fig 3 27 3 Connect the camera to the stand Fig 3 27 7 via the USB Commercial Micro D cable Fig 3 27...

Page 73: ...zer D or filter holder Lift the condenser carrier together with its knurled knob upwards as far as it will go If necessary unscrew the locking and holding pins together with the low power system from...

Page 74: ...he front threaded opening to the left below the condenser carrier 571HFig 3 29 3 until it stops With the adjustment lever 572HFig 3 29 1 screw the stop bolt 573HFig 3 29 2 into the back threaded openi...

Page 75: ...rd into the condenser Make sure that the disk engages in the guide on both inner sides of the condenser The guide serves as a stop for the modulator disk The pin of the disk s clamping screw must slid...

Page 76: ...ake hold of the recessed grips of the knob with your fingertips and turn it to the desired position Pressing the Intensity LM knob initiates further functionality see sections 2 4 8 and 3 5 Select Per...

Page 77: ...taneously for at least 1 5 seconds The indicator light blinks in the following sequence GREEN ORANGE GREEN To enable the LM function proceed as follows Press one of the Snap buttons and the Intensity...

Page 78: ...configuration stored will be cleared To reset the microscope to default factory settings proceed as follows Press and hold down the Intensity LM knob for 20 seconds While the knob is held down from 3...

Page 79: ...the tube viewing angle which may be stationary or variable depending on the model With an inter pupillary distance of 65 mm and a tube viewing angle of 30 the height adjustment range is approximately...

Page 80: ...the eyepiece reticle with the focusable eye lens of the adjustable eyepiece Focus on the microscopic image of a loaded specimen with the focusing drive while observing with the eyepiece containing the...

Page 81: ...ted light brightfield microscopy using the K HLER method 2 Instrumentation for transmitted light brightfield microscopy The equipment of every Axiolab 5 microscope except the stand for reflected light...

Page 82: ...ig 4 4 4 to between 2 3 4 5 of the diameter of the exit pupil of the objective Fig 4 3 E In most applications this aperture diaphragm setting provides optimal contrast at almost ideal resolution and i...

Page 83: ...4 4 1 of the height stop using an Allen wrench AF 3 Use the focusing drive to focus on the specimen Close the luminous field diaphragm and focus it by turning the vertical control Fig 4 4 2 of the co...

Page 84: ...r disk BF DF Ph 1 Ph 2 Ph 3 Condenser achrom aplan 0 9 H D Ph DIC Darkfield condenser with dry darkfield 465505 0000 000 applicable aperture from 0 6 0 75 Ultra condenser 465500 0000 000 applicable ap...

Page 85: ...stop Place the dry darkfield condenser in the condenser holder Z see section 3 1 9 Place the condenser holder Z in the condenser carrier and center it roughly so the condenser fits into the opening of...

Page 86: ...ment screws and focus the image with the condenser drive Place a drop of immersion oil on the specimen holder swivel in the immersion oil objective and focus the specimen Close the field diaphragm dow...

Page 87: ...fluenced partial beams and these strengthen or weaken according to phase position This interference results mainly in image contents displaying differences in intensity which can be perceived by the h...

Page 88: ...ove the auxiliary microscope from the tube and replace it with the eyepiece In order to increase the image contrast an interference broadband filter green 32 x 4 may be mounted on the field diaphragm...

Page 89: ...ppear that range from gray mostly for biological samples through white yellow and red to blue depending on the birefringence thickness and orientation of the sample The interference colors may be of t...

Page 90: ...es not already have an analyzer The field of view will appear dark due to the crossed polarizers With screw on analyzers in the intermediate plate for tubes care must be taken to ensure that they are...

Page 91: ...tary stage Fig 4 7 1 Polarizer D rotatable or fixed Screw in fixed analyzer slider D or lambda compensator or lambda 4 compensator combined with analyzer in Axiolab 5 tubes Pol adjustment tool sample...

Page 92: ...with screw in analyzer in tube or intermediate plate Like the sample the lambda compensator is a birefringent object albeit with a defined path difference of 550 nm and the principal polarization dire...

Page 93: ...rization direction n of the lambda compensator i e in the NE SW direction the path difference of the sample e g gray white 150 nm and the path difference of the lambda compensator red 550 nm add up Th...

Page 94: ...rn the Pol rotary stage until the specimen is almost extinguished i e completely dark and set the 45 locking position Rotate the stage once by 45 so that the specimen is in a diagonal position sample...

Page 95: ...nt specimens display the characteristic interference colors 2 Instrumentation Strain free objectives Pol rotary stage Circular polarizer D no polarizers may be adapted on the condenser including corre...

Page 96: ...nts 45 to the right 1 Slider 6x20mm with lambda 4 plate 2 Lower section of circular polarizer 3 Lever for rotating the lambda 4 plate 4 Lambda 4 plate in the upper part of the circular polarizer 5 Adj...

Page 97: ...el in the lambda plate and if a rotary lambda plate is available e g 445226 0000 000 set the oscillation direction to 45 stop position Select crystal needles that are oriented in the gamma direction s...

Page 98: ...referred to as conoscopy Its main application is classic mineral microscopy However synthetic crystals industrial minerals and plastics e g films can also be identified and characterized 2 Instrument...

Page 99: ...to avoid superimposition of axial figures of neighboring crystals on the axial figure to be examined The smallest crystal value that can be faded out is 170 m Switch on Bertrand lens BL Fig 4 11 1 On...

Page 100: ...g 4 12 Determining optical character In the case of less favourable sections in which the cross hair center is optically uniaxial or the isogyres are optically biaxial specimens outside the objective...

Page 101: ...ring the rotation During the rotation procedure interference colors appear that range from gray mostly for biological samples through white yellow and red to blue depending on the birefringence thickn...

Page 102: ...at 0 Put the analyzer slider Fig 4 13 2 into the beam path and adjust it using the setting wheel until the field of view is dark Bring the specimen to be examined into the field of view and rotate it...

Page 103: ...e with crossline reticle Strain free objectives Pol rotary stage Fig 4 13 1 Polarizer D rotatable or fixed Lambda or lambda 4 compensator as required Pol adjustment tool sample for polarization micros...

Page 104: ...he sample the lambda compensator is a birefringent object albeit with a defined path difference of 550 nm and the principal polarization direction n definitely oriented in a NE SW direction When the l...

Page 105: ...erence 700 nm If the polarization direction of the birefringence sample to be examined is perpendicular to the principal polarization direction of the lambda compensator i e in the NW SE direction the...

Page 106: ...side color changes from light gray white to a strong interference color when a lambda compensator 473704 0000 000 is inserted in the compensator slot the path difference is 1 4 1 2 NOTE A prerequisit...

Page 107: ...Configuring the microscope Configure the microscope as for transmitted light brightfield microscopy using the K HLER method see section 4 2 1 Center the Pol rotary stage or objective if this has not a...

Page 108: ...fter the above adjustment has been completed Reinsert the specimen to be examined The interference colors which depend on the material specimen thickness and orientation of the specimens appear consta...

Page 109: ...4 2 6 1 Application Crystal analysis is used to determine the optical character of transparent and weakly absorbent crystals This method is also referred to as conoscopy Its main application is classi...

Page 110: ...ally set to the On if it is not already there If on the other hand rotary knob A is set to the Off position rotary knob BL will be automatically set to the Off position if it is not already there Plac...

Page 111: ...ptically uniaxial Optically biaxial Positive Negative Positive Negative Lambda plate white blue yellow Blue Yellow Quartz wedge Direction of motion at insertion Direction of movement Lambda 4 plate po...

Page 112: ...only with the stand for reflected light P C ACR brightfield reflector module for reflected light in the reflector turret 3 Configuring reflected light brightfield microscopy The microscope has been st...

Page 113: ...the best compromise for the human eye Then reinsert the eyepiece adjust the focus with the coaxial coarse and fine focusing knobs and adjust the brightness to the reflected light specimen Readjust ap...

Page 114: ...flected light Epiplan Neofluar EC Epiplan Neofluar Epiplan objectives with the additional designation HD ACR P C darkfield reflector module for reflected light NOTE The stand for reflected light is eq...

Page 115: ...men surfaces which otherwise would be unavoidable 2 Instrumentation Observations in the reflected light darkfield can be made only on Axiolab 5 microscopes for reflected light Pol rotary stage Epiplan...

Page 116: ...nally the beams pass through a band elimination filter longpass bandpass which only permits the long wave radiation emitted by the specimen to pass through The spectra of the excitation and the band e...

Page 117: ...by pushing the TL button Fig 4 20 5 If necessary turn the condenser turret Fig 4 20 7 to the BF position for transmitted light brightfield or phase contrast if using a Ph objective and seek the speci...

Page 118: ...selection knob for swiveling in the LED UV 385 nm or LED B 475 nm or LED G 555 nm 2 Nosepiece 3 Intensity LM knob 4 Reflected light button RL 5 Transmitted light button TL 6 Focusing drive 7 Condenser...

Page 119: ...is made of 90 vol petroleum ether and 10 vol isopropyl alcohol IPA The components are also known by the following names Petroleum ether surgical spirit benzine Isopropanol alcohol 2 propyl alcohol di...

Page 120: ...disconnect the instrument from the power supply before replacing fuses If the fuses fail the reason must first of all be ascertained and technical problems properly remedied The fuse box is located a...

Page 121: ...luminous field diaphragm correctly aperture see p 81 ff The filter has not been inserted correctly in the filter mount Insert filter correctly in the filter mount Low resolving power and poor image c...

Page 122: ...stance inter pupillary distance is not adjusted correctly Adjust the inter pupillary distance correctly see p 79 Focusable eyepieces are not adjusted correctly Adjust the focusable eyepieces according...

Page 123: ...nt mains power socket Halogen lamp flickers illumination intensity is not stable Halogen lamp is reaching the end of its service life Replace the halogen lamp see p 64 Power cable is not installed pro...

Page 124: ...D under Operating System setting The buffer battery has no charge Contact service The image has severe noise The amplification gain is set too high Open 3D noise reduction reduce the gain The exposure...

Page 125: ...the distortion Set the monitor aspect ratio to 16 9 The image is blurred on the screen but the sample is in focus through eyepieces Focus plane of the camera is different from that of the eyepieces Ma...

Page 126: ...of time we recommend that you enter into a service maintenance agreement with ZEISS For subsequent orders or when service is required please get in touch with your local ZEISS representative If servic...

Page 127: ...Deutsches Institut f r Normung German Institute for Standardization EC European Community EN Euronorm European standard Ergo Ergonomic Ergonomics FL Fluorescence foc focusable GW Suitability for glass...

Page 128: ...ons Axiolab 5 128 430037 7444 001 05 2019 SLR Single Lens Reflex T Slow type of fuse TIC Total interference contrast in circular polarized light TL Transmitted light UL Underwriters Laboratories UV Ul...

Page 129: ...gence 89 101 Bright field 81 112 C Cable holder 22 24 26 28 30 32 Care 119 Carrying handle 22 26 28 Centering screw for condenser 22 24 26 28 30 32 Checking the instrument 120 Circular polarization co...

Page 130: ...ected light 30 32 Fine adjustment 22 24 26 28 30 32 Firmware update 126 Fluorescence 24 116 Focusing drive 22 24 26 28 30 32 83 Friction adjustment 58 Front lens 62 81 H Height stop on condenser carri...

Page 131: ...94 Permanent ECO mode switch 22 24 26 32 Phase contrast 87 Photo tube 22 26 28 30 32 35 53 Pinhole diaphragm 54 Polarization 26 28 30 89 98 109 115 Polarization direction 91 103 Polarizer 21 42 73 90...

Page 132: ...28 30 32 Transmitted light 22 24 26 28 30 32 81 87 89 98 109 Transmitted light TL button 24 30 32 Transmitted light illumination 24 26 28 Transmitted light illuminator 22 30 32 Transmitted light phas...

Page 133: ...r Video 60 C 1 3 0 4x 456108 0000 000 Slider with beam splitter 426141 9021 000 Slider with 100 mirror 426141 9011 000 Double adapter 60N 2x 60N 1 0x 426141 9902 000 Slider 12x46 with focusing Bertran...

Page 134: ...424941 9000 000 Optovar modules see price list Analyzer slide fixed for transmitted light 6x20 433605 0000 000 Lambda 4 compensator 6x20 473714 0000 000 Analyzer slide D fixed with lambda plate 45368...

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