Image Acquisition
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Activate this function if you notice, e.g. with bright-field images, a reduction
in image brightness, caused by the optics, at the image edge. For this
calibration process make sure that the camera is viewing a completely empty
image. To ensure that this is the case, move your sample on the microscope
completely out of the field of view.
Shading correction to improve image quality
In microscopy, numerous side effects can have an undesirable influence on
image quality. Dust and particles in the beam path, for example, can leave
dark spots in the image, and, under certain conditions, diaphragms and optics
can cause slight shadowing that increases towards the image edge, due to
unavoidable physical effects.
For all AxioCam models it is possible to acquire a shading reference image and
apply this automatically each time an image is acquired. On the camera
property page
General
you will find the
Shading Correction
button. This
can be used to trigger the acquisition and saving of a shading reference
image.
If you are using a motorized microscope or a microscope with an encoded
nosepiece, it is possible to acquire a separate reference image for each
objective. These images are then applied automatically for the objective that
has been selected.
Procedure for generating objective-specific shading correction
Prerequisites:
•
Encoded or motorized microscope stand
•
Camera belonging to the AxioCam family
The following steps are required to set and apply shading correction in the
optimum manner:
¾
Activate the
Automatic
check box on the camera property page
General
.
AxioCam MR Instal Reference
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